Summary
Loss of antigenicity upon fixation has been a problem in immunocytochemistry. We previously reported [J Cell Biol (1984) 99: 2061–2073] on a new fixation technique using photoactivated 1,3,5-triazido-2,4,6-trinitrobenzene (TTB). This fixative preserves antigenicity in general better than the many conventional fixatives we have tried. We communicate here on the existence and some properties of a whole host of structurally related compounds, many of which can also act as immunocytochemical fixatives upon irradiation. Although TTB still produces the best antigenic preservation of all the compounds tested, certain of the compounds have special properties, such as water solubility and the ability to be rapidly photoactivated, which allow them to be used in a wider variety of situations.
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Abbreviations
- TTB:
-
1,3,5-triazido-2,4,6-trinitrobenzene
- IgG:
-
immunoglobulin G
- TNB:
-
1,3,5-trinitrobenzene
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Dedicated to Professor Dr. Noburo Kamiya on the occasion of his 75th birthday
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© 1988 Springer-Verlag
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McBeath, E., Fujiwara, K. (1988). A New Class of Photoactivated Fixatives for Immunocytochemistry. In: Tazawa, M. (eds) Cell Dynamics. Protoplasma, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9011-1_14
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DOI: https://doi.org/10.1007/978-3-7091-9011-1_14
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9013-5
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