Summary
A preparation of phragmoplasts with a purity of about 40% was isolated from tobacco BY-2 cells whose cell cycle was synchronized by treatment with aphidicolin and propyzamide. Each isolated phragmoplast was associated with the daughter nuclei and the association was maintained after the phragmoplast lost either its actin filaments or its microtubules. Actin filaments identified by heavy meromyosin arrowhead decoration were abundant in the isolated phragmoplasts. Most of the filaments were oriented perpendicularly or nearly perpendicularly to the equatorial plate and in about 80% of the filaments thus oriented heavy meromyosin arrowheads pointed away from the plate. Abundant vesicles were present in the isolated phragmoplast-nuclei complex and they were associated with phragmoplast microtubules.
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Abbreviations
- 2,4-D:
-
4-dichlorophenoxyacetic acid
- DAPI:
-
4’,6’diamidino-2-phenylindole
- DMSO:
-
dimethylsulfoxide
- EGTA:
-
ethyleneglycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid
- PIPES:
-
piperazine-N,N’-bis(2-ethanesulfonic acid)
- PMSF:
-
phenylmethylsulfonyl fluoride
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Dedicated to Professor Dr. Noburo Kamiya on the occasion of his 75th birthday
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© 1988 Springer-Verlag
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Kakimoto, T., Shibaoka, H. (1988). Cytoskeletal Ultrastructure of Phragmoplast-Nuclei Complexes Isolated from Cultured Tobacco Cells. In: Tazawa, M. (eds) Cell Dynamics. Protoplasma, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9011-1_11
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DOI: https://doi.org/10.1007/978-3-7091-9011-1_11
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9013-5
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