Skip to main content

Part of the book series: Plant Gene Research ((GENE))

Abstract

The phenomenon tumor formation on plants has intrigued biologists for a long time. More than 75 years ago, it was reported that a particular type of plant tumor, known as crown gall, was caused by bacteria (Smith and Townsend, 1907). The present taxonomic name of this soil bacterium is Agrobacterium tumefaciens. Crown gall is characterized by unlimited cell proliferations, which may lead to regression or even to death of the plant. The disease and its causative agent have been studied intensively, first to uncover concepts underlying the tumorous state, secondly because it is the cause of losses of economically important plants in Europe (Kerr and Panagopoulos, 1977), North-America (Kennedy and Alcorn, 1980), and Australia (New and Kerr, 1972), and thirdly, more recently, to develop plant gene vectors.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bomhoff, G., Klapwijk, P. M., Kester, H. C. M., Schilperoort, R. A., Hernalsteens, J. P., Schell, J., 1976: Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens. Mol. Gen. Genet. 145, 177–181.

    PubMed  CAS  Google Scholar 

  • Braun, A. C., 1943: Studies on tumor inception in the crown-gall disease. Am. J. Bot. 30, 674–677.

    Google Scholar 

  • Braun, A. C., 1947: Thermal studies on the factors responsible for tumor initiation in crown gall. Am. J. Bot. 34, 234–240.

    PubMed  CAS  Google Scholar 

  • Braun, A. C., 1956: The activation of two growth-substance systems accompanying the conversion of normal to tumor cells in crown gall Cancer Res. 16, 53–56.

    CAS  Google Scholar 

  • Braun, A. C., White, P. R., 1943: Bacteriological sterility of tissues derived from secondary crown gall tumors. Phytopathology 33, 85–100.

    CAS  Google Scholar 

  • Breathnach, R., Chambon, P., 1981: Organization and expression of eukaryotic split genes coding for proteins. Ann. Rev. Biochem. 50, 349–383.

    PubMed  CAS  Google Scholar 

  • Chilton, M.-D., Farrand, S. K., Levin, R. L., Nester, E. W., 1976: RP4 promotion of a large Agrobacterium plasmid which confers virulence. Genetics 83, 609–618.

    PubMed  CAS  Google Scholar 

  • Chilton, M.-D., Drummond, M. H., Merlo, D. J., Sciaky, D., Montoya, A. L., Gordon, M. P., Nester, E. W., 1977: Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11, 263–271.

    PubMed  CAS  Google Scholar 

  • Chilton, M.-D., Montoya, A. L., Merlo, D. J., Drummond, M. H., Nutter, R., Gordon, M. P., Nester, E. W., 1978a: Restriction endonuclease mapping of a plasmid that confers oncogenicity upon Agrobacterium tumefaciens strain B 6–806. Plasmid 1, 254–269.

    PubMed  CAS  Google Scholar 

  • Chilton, M.-D., Drummond, M. H., Merlo, D. J., Sciaky, D., 1978 Highly conserved DNA of Ti-plasmids overlaps T-DNA maintained in plant tumors. Nature 275, 147–149.

    CAS  Google Scholar 

  • Chilton, M.-D., Saiki, R. K., Yadav, N., Gordon, M. P., Quetier, F., 1980: T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells. Proc. Natl. Acad. Sci. U. S. A. 77, 4060–4064.

    PubMed  CAS  Google Scholar 

  • Costantino, P., Hooykaas, P. J. J., Den Dulk-Ras, H., Schilperoort, R. A., 1980: Tumor formation and rhizogenicity of Agrobacterium rhizogenes carrying Ti plasmids. Gene 11, 79–87.

    PubMed  CAS  Google Scholar 

  • Costantino, P., Mauro, M. L., Micheli, G., Risuleo, G., Hooykaas, P. J. J., Schilperoort, R. A., 1981: Fingerprinting and sequence homology of plasmids from different virulent strains of Agrobacterium rhizogenes. Plasmid 5, 170–182.

    PubMed  CAS  Google Scholar 

  • Currier, T. C., Nester, E. W., 1976: Evidence for diverse types of large plasmids in tumor-inducing strains of Agrobacterium. J. Bacteriol. 126, 157 165.

    Google Scholar 

  • De Greve, H., Decraemer, H., Seurinck, J., Van Montagu, M., Schell, J., 1981: The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB 6S3. Plasmid 6, 235–248.

    PubMed  Google Scholar 

  • De Greve, H., Dhaese, P., Seurinck, J., Lemmers, M., Van Montagu, M., Schell, J., 1983: Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti-plasmid encoded octopine synthase gene. J. Mol. Appl. Genet. 2, 499–511.

    Google Scholar 

  • De Picker, A. Van Montagu, M., Schell, J., 1978: Homologous DNA sequences in different Tiplasmids are essential for oncogenicity. Nature 275, 150–153.

    Google Scholar 

  • De Picker, A., De Wilde, M., De Vos, G., De Vos, R., Van Montagu, M., Schell, J., 1980: Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping. Plasmid 3, 193–211.

    Google Scholar 

  • De Picker, A., Stachel, S., Dhaese, P., Zambryski, P., Goodman, H. M., 1982: Nopaline synthase: transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1, 561–573.

    Google Scholar 

  • De Vos, G., De Beuckeleer, M., Van Montagu, M., Schell, J., 1981: Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch 5 of Agrobacterium tumefaciens. Plasmid 6, 249–253.

    PubMed  Google Scholar 

  • Drummond, M. H., Chilton, M.-D., 1978: Tumor-inducing (Ti) plasmids of Agrobacterium share extensive regions of DNA homology. J. Bacteriol. 136, 1178–1183.

    PubMed  CAS  Google Scholar 

  • Drummond, M. H., Gordon, M. P., Nester, E. W., Chilton, M.-D., 1977: Foreign DNA of bacterial plasmid origin is transcribed in crown gall tumours. Nature 269, 535–536.

    CAS  Google Scholar 

  • Ellis, J. G., Murphy, P. J. 1981: Four new opines from crown gall tumours — their detection and properties. Mol. Gen. Genet. 181, 36–43.

    CAS  Google Scholar 

  • Ellis, J. G., Kerr, A., TempĂ©, J., Petit, A., 1979: Arginine catabolism: a new function of both octopine and nopaline Ti-plasmids of Agrobacterium. Mol. Gen. Genet. 173, 263–269.

    PubMed  CAS  Google Scholar 

  • Ellis, J. G., Kerr, A., Petit, A., TempĂ©, J., 1982a: Conjugal transfer of nopaline and agropine Ti-plasmids. The role of agrocinopines. Mol. Gen. Genet. 186, 269–274.

    CAS  Google Scholar 

  • Ellis, J. G., Murphy, P. J., Kerr, A., 1982b: Isolation and properties of transfer regulatory mutants of the nopaline Ti-plasmid pTiC 58. Mol. Gen. Genet. 186, 275–281.

    CAS  Google Scholar 

  • Engler, G., De Picker, A., Maenhout, R., Villarroel, R., Van Montagu, M., Schell, J. (1981). Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti-plasmid of Agrobacterium tumefaciens. J. Mol. Biol. 152, 183–208.

    PubMed  CAS  Google Scholar 

  • Firmin, J. L., Fenwick, G. R., 1978: Agropine: a major new plasmid-determined metabolite in crown gall tumours. Nature 276, 842–844.

    CAS  Google Scholar 

  • Garfinkel, D. J., Nester, E. W., 1980: Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J. Bacteriol. 144, 732–743.

    PubMed  CAS  Google Scholar 

  • Garfinkel, D. J., Simpson, R. B., Ream, L. W., White, F. F., Gordon, M. P., Nester, E. W., 1981: Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell 27, 143–153.

    PubMed  CAS  Google Scholar 

  • Gelvin, S. B., Thomashow, M. F., McPherson, J. C., Gordon, M. P., Nester, E. W., 1982: Sizes and map positions of several plasmid DNA-encoded transcripts in octopine-type crown gall tumors. Proc. Natl. Acad. Sci. U. S. A. 79, 76–80.

    PubMed  CAS  Google Scholar 

  • Genetello, C., Van Larebeke, N., Holsters, M., De Picker, A., Van Montagu, M., Schell, J., 1977: The Ti-plasmids of Agrobacterium as conjugative plasmids. Nature 265, 561–563.

    PubMed  CAS  Google Scholar 

  • Goldmann, A., Thomas, D. W., Morel, G., 1969: Sur la structure de la nopaline, mĂ©tabolite anormale de certaines tumeurs de crown gall. C. R. Acad. Sci. Paris 268, 852–854.

    CAS  Google Scholar 

  • Gurley, W. B., Kemp, J. D., Albert, M. J., Sutton, D. W., Callis, J., 1979: Transcription of Ti-plasmid derived sequences in the octopine type crown gall tumor lines. Proc. Natl. Acad. Sci. U. S. A. 76, 2828–2832.

    PubMed  CAS  Google Scholar 

  • Hamilton, R. H., Fall, M. Z., 1971: The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia 27, 229–230.

    PubMed  CAS  Google Scholar 

  • Heidekamp, F., Dirkse, W. G., Hille, J., Van Ormondt, H., Schilperoort, R. A., De Groot, B., 1983: Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti-plasmid encoded tmr gene. Nucl. Ac. Res., 11, 6211–6223.

    CAS  Google Scholar 

  • Hepburn, A. G., Hindley, J., 1979: Regions of DNA sequence homology between an octopine and a nopaline Ti-plasmid of Agrobacterium tumefaciens. Mol. Gen. Genet. 169, 163–172.

    CAS  Google Scholar 

  • Hernalsteens, J. P., De Greve, H., Van Montagu, M., Schell, J., 1978: Mutagenesis by insertion of the drug resistance transposon Tn7 applied to the Ti plasmid of Agrobacterium tumefaciens. Plasmid 1, 218–225.

    PubMed  CAS  Google Scholar 

  • Hille, J., Schilperoort, R., 1981: The use of transposons to introduce well-defined deletions in plasmids: possibilities for in vivo cloning. Plasmid 6, 151–154.

    PubMed  CAS  Google Scholar 

  • Hille, J., Klasen, I., Schilperoort, R., 1982: Construction and application of R-prime plasmids, carrying different segments of an octopine Ti-plasmid from Agrobacterium tumefaciens for complementation of vir genes. Plasmid 7, 107–118.

    PubMed  CAS  Google Scholar 

  • Hille, J., Van Kan, J., Klasen, I., Schilperoort, R., 1983a: Site-directed mutagenesis in Escherichia coli of a stable R772: Ti cointegrate plasmid from Agrobacterium tumefaciens. J. Bacteriol. 154, 693–701.

    PubMed  CAS  Google Scholar 

  • Hille, J., Van Kan, J., Schilperoort, R. 1983: Onc-and vir-genes located on the Tiplasmid of Agrobacterium tumefaciens. In: Proceedings of molecular genetics of the bacteria-plant interaction. PĂ¼hler, A. (ed.), pp. 223–220, Berlin-Heidelberg-New York: Springer.

    Google Scholar 

  • Hille, J., Wullems, G., Schilperoort, R., 1983: Non-oncogenic T-region mutants of Agrobacterium tumefaciens do transfer T-DNA into plant cells. Pl. Mol. Biol., 2, 155–163.

    CAS  Google Scholar 

  • Hille, J., et al.,1983d: in preparation.

    Google Scholar 

  • Hoekema, A., Hirsch, P. R., Hooykaas, P. J. J., Schilperoort, R. A., 1983 A binary plant vector strategy based on separation of the Vir-and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303, 179–180.

    CAS  Google Scholar 

  • Hoekema, A. Van Haaren, M. J. J., Hille, J., Hoge, J. H. C., Hooykaas, P. J. J., Krens, F. A., Wullems, G. J., Schilperoort, R. A., 1983: Agrobacterium tumefaciens and its Ti-plasmid as tools in transformation of plant cells. In: Plant Molecular Biology. Goldberg, R. B. (ed.), New York: Alan R. Liss.

    Google Scholar 

  • Holsters, M., De Waele, D., De Picker, A., Messens, E., Van Montagu, M., Schell, J., 1978: Transfection and transformation of Agrobacterium tumefaciens. Mol. Gen. Genet. 163, 181–187.

    PubMed  CAS  Google Scholar 

  • Holsters, M., Silva, B., Van Vliet, F., Genetello, C., De Block, M., Dhaese, P., De Picker, A., InzĂ©, D., Engler, G., Vilarroel, R., Van Montagu, M., Schell, J., 1980: The functional organization of the nopaline A. tumefaciens plasmid pTiC 58. Plasmid 3, 212–230.

    PubMed  CAS  Google Scholar 

  • Hooykaas, P. J. J., 1979: The role of plasmid determined functions in the interactions of Rhizobiaceae with plant cells. A genetic approach, 168 pp. Thesis, Leiden, The Netherlands.

    Google Scholar 

  • Hooykaas, P. J. J., Schilperoort, R. A., 1983: The molecular genetics of crown gall tumorigenesis. In: Advances in Genetics. Scandalios, J. G. (ed.), New York: Academic Press.

    Google Scholar 

  • Hooykaas, P. J. J., Klapwijk, P. M., Nuti, M. P., Schilperoort, R. A., Rörsch, A., 1977: Transfer of the Agrobacterium tumefaciens Ti-plasmid to avirulent agrobacteria and to Rhizobium ex planta. J. Gen. Microbiol. 98, 477–484.

    Google Scholar 

  • Hooykaas, P. J. J., Roobol, C., Schilperoort, R. A., 1979: Regulation of the transfer of Ti-plasmids of Agrobacterium tumefaciens. J. Gen. Microbiol. 110, 99–109.

    CAS  Google Scholar 

  • Hooykaas, P. J. J., Den Dulk-Ras, H., Schilperoort, R. A., 1980: Molecular mechanism of Ti-plasmid mobilization by R-plasmids. Isolation of Ti-plasmids with transposon-insertions in Agrobacterium tumefaciens. Plasmid 4, 65–74.

    Google Scholar 

  • Hooykaas, P. J. J., Den Dulk-Ras, H., Ooms, G., Schilperoort, R. A., 1980: Interactions between octopine and nopaline plasmids in Agrobacterium tumefaciens. J. Bacteriol. 143, 1295–1306.

    PubMed  CAS  Google Scholar 

  • Iyer, V. N., Klee, H. J., Nester, E. W. 1982: Units of genetic expression in the virulence region of a plant tumor-inducing plasmid of Agrobacterium tumefaciens. Mol. Gen. Genet. 188, 418–424.

    PubMed  CAS  Google Scholar 

  • Kennedy, B. W., Alcorn, S. M., 1980: Estimates of U. S. A. crop losses to prokaryotic plant pathogens. Plant Dis. 64, 674–676.

    Google Scholar 

  • Kerr, A. 1969: Transfer of virulence between isolates of Agrobacterium. Nature 223, 1175–1176.

    Google Scholar 

  • Kerr, A., 1971: Acquisition of virulence by non-pathogenic isolates of Agrobacterium radiobacter. Physiol. Plant Pathol. 1, 241–246.

    Google Scholar 

  • Kerr, A., 1972: Biological control of crown gall: seed inoculation. J. appl. Bact. 35, 493–497.

    Google Scholar 

  • Kerr, A., 1980: Biological control of crown gall through production of agrocin 84. Plant Dis. 64, 25–30.

    Google Scholar 

  • Kerr, A., Panagopoulos, C. G., 1977: Biotypes of Agrobacterium radiobacter var. tumefaciens and their biological control. Phytopath. Z. 90, 172–179.

    Google Scholar 

  • Kerr, A., Manigault, P., TempĂ©, J., 1977: Transfer of virulence in vivo and in vitro in Agrobacterium. Nature 265, 560–561.

    PubMed  CAS  Google Scholar 

  • Klapwijk, P. M., 1979: The role of octopine in conjugation and plant tumour formation by Agrobacterium tumefaciens. Studies on tumour-inducing plasmids. Thesis, Leiden, The Netherlands, 128 pp.

    Google Scholar 

  • Klapwijk, P. M., Schilperoort, R. A., 1979: Negative control of octopine degradation and transfer genes of octopine Ti-plasmids in Agrobacterium tumefaciens. J. Bacteriol. 139, 424–431.

    PubMed  CAS  Google Scholar 

  • Klapwijk, P. M., Schilperoort, R. A., 1982: Genetic determination of octopine degradation. In: Molecular Biology of Plant Tumors. Kahl, G., Schell, J. (eds.), pp. 475–495. New York: Academic Press.

    Google Scholar 

  • Klapwijk, P. M., Scheulderman, T., Schilperoort, R. A., 1978: Coordinated regulation of octopine degradation and conjugative transfer of Ti-plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons. J. Bacteriol. 136, 775–785.

    PubMed  CAS  Google Scholar 

  • Klee, H. J., Gordon, M. P., Nester, E. W., 1982: Complementation analysis of Agrobacterium tumefaciens Ti-plasmid mutations affecting oncogenicity. J. Bacteriol. 150, 327–331.

    PubMed  CAS  Google Scholar 

  • Klee, H. J., White, F. F., Iyer, V. N., Gordon, M. P., Nester, E. W., 1983: Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti-plasmid. J. Bacteriol. 153, 878–883.

    PubMed  CAS  Google Scholar 

  • Knauf, V. C., Nester, E. W., 1982: Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti-plasmid. Plasmid 8, 45–54.

    PubMed  CAS  Google Scholar 

  • Koekman, B. P., Ooms, G., Klapwijk, P. M., Schilperoort, R. A., 1979: Genetic map of an octopine Ti-plasmid. Plasmid 2, 347–357.

    PubMed  CAS  Google Scholar 

  • Koekman, B. P., Hooykaas, P. J. J., Schilperoort, R. A., 1980: Localization of the replication control region on the physical map of the octopine Ti-plasmid. Plasmid 4, 184–195.

    PubMed  CAS  Google Scholar 

  • Koekman, B. P., Hooykaas, P. J. J., Schilperoort, R. A., 1982: A functional map of the replicator region of the octopine Ti-plasmid. Plasmid 7, 119–132.

    PubMed  CAS  Google Scholar 

  • Krens, F. A., Molendijk, L., Wullems, G. J., Schilperoort, R. A., 1982: In vitro trans- formation of plant protoplasts with Ti-plasmid DNA. Nature 296, 72–74.

    CAS  Google Scholar 

  • Ledeboer, A. M., 1978: Large plasmids in Rhizobiaceae. Studies on the transcription of the tumour inducing plasmid from Agrobacterium tumefaciens in sterile crown gall tumour cells, pp. 180, Thesis, University of Leiden, The Netherlands.

    Google Scholar 

  • Leemans, J., Deblaere, R., Willmitzer, L., De Greve, H., Hernalsteens, J. P., Van Montague, M., Schell, J., 1982: Genetic identification of fuctions of TL-DNA transcripts in octopine crown galls. EMBO J. 1, 147–152.

    PubMed  CAS  Google Scholar 

  • Liu, S. T., Perry, K. L., Schardl, C. L., Kado, C. I., 1982: Agrobacterium Ti-plasmid indoleacetic acid gene is required for crown gall oncogenesis. Proc. Natl. Acad. Sci. U. S. A. 79, 2812–2816.

    PubMed  CAS  Google Scholar 

  • Loper, J. E., Kado, C. I., 1979: Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens. J. Bacteriol. 139, 591–596.

    PubMed  CAS  Google Scholar 

  • Matzke, A. J. M., Chilton, M.-D., 1981: Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: An approach to genetic engineering of higher plant cells. J. Mol. Appl. Genet. 1, 39–49.

    PubMed  CAS  Google Scholar 

  • McKenney, K., Shimatake, H., Court, D., Schmeissner, U., Brady, C., Rosenberg, M., 1981: A system to study promoter and terminator signals recognized by E. coli RNA polymerase. In: Gene amplification and analysis. Vol. 2, pp. 383–415. Chrikjian, J. G., Papas, T. S. (eds.). Elsevier/North Holland.

    Google Scholar 

  • MĂ©nagĂ©, A., Morel, G., 1964: Sur la prĂ©sence d’octopine dans les tissus de crown-gall. C. R. Acad. Sci (Paris) 259D, 4795–4796.

    Google Scholar 

  • Moore, L., Warren, G., Strobel, G., 1979: Involvement of a plasmid in the hairy root-disease of plants caused by Agrobacterium rhizogenes. Plasmid 2, 617–626.

    PubMed  CAS  Google Scholar 

  • Murai, N., Kemp, J. D., 1982: T-DNA of pTi 15955 from Agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated RNAs in a sunflower crown gall tumor. Nucl. Ac. Res. 10, 1679–1689.

    CAS  Google Scholar 

  • Murphy, P. J., Tate, M. E., Kerr, A., 1981: Substituents at N6 and C-5’ control selective uptake and toxicity of the adenine-nucleotide bacteriocin, agrocin 84, in agrobacteria. Eu. J. Biochem. 115, 539–543.

    CAS  Google Scholar 

  • New, P. B., Kerr, A., 1971: A selective medium for Agrobacterium radiobacter biotype 2. J. appl. Bact. 34, 233–236.

    CAS  Google Scholar 

  • New, P. B., Kerr, A., 1972: Biological control of crown gall: field observations and glasshouse experiments. J. appl. Bact. 35, 279–287.

    Google Scholar 

  • Ooms, G., Klapwijk, P. M., Poulis, J. A., Schilperoort, R. A., 1980: Characterization of Tn 904 insertions in octopine Ti-plasmid mutants of Agrobacterium tumefaciens. J. Bacteriol. 144, 82–91.

    PubMed  CAS  Google Scholar 

  • Ooms, G., Hooykaas, P. J. J., Moolenaar, G., Schilperoort, R. A., 1981: Crown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti-plasmids; analysis of T-DNA functions. Gene 14, 33–50.

    PubMed  CAS  Google Scholar 

  • Ooms, G., Hooykaas, P. J. J., Van Veen, R. J. M., Van Beelen, P., RegensburgTuĂ¯nk, A. J. G., Schilperoort, R. A., 1982: Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7, 15–29.

    PubMed  CAS  Google Scholar 

  • Panagopoulos, C. G., Psallidas, P. G., 1973: Characteristics of Greek isolates of Agrobacterium tumefaciens (Smith and Townsend) Conn. J. appl. Bact. 36, 233–240.

    CAS  Google Scholar 

  • Petit, A., TempĂ©, J., 1978: Isolation of Agrobacterium Ti-plasmid regulatory mutants. Mol. Gen. Genet. 167, 147–155.

    CAS  Google Scholar 

  • Petit, A., Delhaye, S., TempĂ©, J., Morel, G., 1970: Recherches sur les guanidines des tissus de crown gall. Mise en Ă©vidence d’une relation biochimique spĂ©cifique entre les souches d’ Agrobacterium et les tumeurs qu’elles induisent. Physiol. Veg. 8, 205–213.

    CAS  Google Scholar 

  • Petit, A., TempĂ©, J., Kerr, A., Holsters, M., Van Montagu, M., Schell, J., 1978: Substrate induction of conjugative activity of Agrobacterium tumefaciens Ti-plasmids. Nature 271, 570–572.

    CAS  Google Scholar 

  • Prakash, R. K., Schilperoort, R. A, 1982: Relationship between nif plasmids of fastgrowng Rhizobium species and Ti-plasmids of Agrobacterium tumefaciens. J. Bacteriol. 149, 1129–1134.

    PubMed  CAS  Google Scholar 

  • Ream, L. W., Gordon, M. P., Nester, E. W., 1983: Multiple mutations in the T-region of the Agrobacterium tumefaciens tumor-inducing plasmid. Proc. Natl. Acad. Sci. U. S. A. 80, 1660–1664.

    PubMed  CAS  Google Scholar 

  • Regier, D. A., Morris, R. O., 1982: Secretion of trans-zeatin by Agrobacterium tumefaciens: a function determined by the nopaline Ti-plasmid. Biochem. Biophys. Res. Commun. 104, 1560–1566.

    PubMed  CAS  Google Scholar 

  • Riker, A. J., 1930: Studies on infectious hairy root of nursery apple trees. J. Agr. Res. 41, 507–540.

    Google Scholar 

  • Risuleo, G., Battistoni, P., Costantino, P., 1982: Regions of homology between tumorigenic plasmids from Agrobacterium rhizogenes and Agrobacterium tumefaciens. Plasmid 7, 45–51.

    PubMed  CAS  Google Scholar 

  • Schilperoort, R. A., Kester, H. C. M., Klapwijk, P. M., Rörsch, A., Schell, J., 1975: Plant tumors induced by A. tumefaciens. A genetic approach. In: Semaine d’étude agriculture et hygiène des plantes. Martens, P. (ed.), pp. 25–35, Gem-blouse, Belgium.

    Google Scholar 

  • Schröder, G., Schröder, J., 1982: Hybridization selection and translation of T-DNA encoded mRNAs from octopine tumors. Mol. Gen. Genet. 185, 51–55.

    Google Scholar 

  • Schröder, J., Schröder, G., Huisman, H., Schilperoort, R. A., Schell, J., 1981: The mRNA for lysopine dehydrogenase in plant tumor cells is complementary to a Ti-plasmid fragment FEBS Lett. 129, 166–168.

    Google Scholar 

  • Smith, E. F., Townsend, C. O., 1907: A plant tumor of bacterial origin. Science 25, 671–673.

    PubMed  CAS  Google Scholar 

  • TempĂ©, J., Petit, A., 1982: Opine utilization by Agrobacterium. In: Molecular Biology of Plant Tumors. Kahl, G., Schell, J. (eds.), pp. 451–459, New York: Academic Press.

    Google Scholar 

  • Thomashow, M. F., Nutter, R., Montoya, A. L., Gordon, M. P., Nester, E. W., 1980a: Integration and organization of Ti plasmid sequences in crown gall tumors. Cell 19, 729–739.

    PubMed  CAS  Google Scholar 

  • Thomashow, M. F., Nutter, R., Postle, K., Chilton, M.-D., Blattner, F. R., Powel, A., Gordon, M. P., Nester, E. W., 1980: Recombination between higher plant DNA and the Ti-plasmid of Agrobacterium tumefaciens. Proc. Natl. Acad. Sci. U. S. A. 77, 6448–6452.

    PubMed  CAS  Google Scholar 

  • Thomashow, M. F., Panagopoulos, C. G., Gordon, M. P., Nester E. W., 1980: Host range of Agrobacterium tumefaciens is determined by the Ti plasmid. Nature 283, 794–796.

    Google Scholar 

  • Thomashow, M. F., Knauf, V. C., Nester, E. W., 1981: The relationship between the limited and wide host range octopine-type Ti plasmids of Agrobacterium tumefaciens. J. Bacteriol. 146, 484–493.

    PubMed  CAS  Google Scholar 

  • Van Larebeke, N., Engler, G., Holsters, M., Van den Elsacker, S., Zaenen, I., Schilperoort, R. A., Schell, J., 1974: Large plasmid in Agrobacterium tumefaciens essential for crown-gall inducing ability. Nature 252, 169–170.

    PubMed  Google Scholar 

  • Van Larebeke, N., Genetello, C., Schell, J., Schilperoort, R. A., Hermans, A. K., Hernalsteens, J. P., Van Montagu, M., 1975: Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature 255, 742–743.

    PubMed  Google Scholar 

  • Van Larebeke, N., Genetello, G., Hernalsteens, J. P., De Picker, A., Zaenen, I., Messens, E., Van Montagu, M., Schell, J., 1977: Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4. Mol. Gen. Genet. 152, 119–124.

    Google Scholar 

  • Vervliet, G., Holsters, M., Teuchy, H., Van Montagu, M., Schell, J., 1975: Characterization of different plaque-forming and defective temperate phages in Agrobacterium strains.

    Google Scholar 

  • Watson, B., Currier, T. C., Gordon, M. P., Chilton, M.-D., Nester, E. W., 1975: Plasmid required for virulence of Agrobacterium tumefaciens. J. Bacteriol. 123, 255–264.

    PubMed  CAS  Google Scholar 

  • White, F. F., Nester, E. W., 1980a: Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes. J. Bacteriol. 141, 1134–1141.

    PubMed  CAS  Google Scholar 

  • White, F. F., Nester, E. W., 1980: Relationship of plasmids responsible for hairy root and crown gall tumorigenicity. J. Bacteriol. 144, 710–720.

    PubMed  CAS  Google Scholar 

  • Willmitzer, L., De Beuckeleer, M., Lemmers, M., Van Montagu, M., Schell, J., 1980: DNA from Ti plasmid present in nucleus and absent from plastids of crown gall plant cells. Nature 287, 359–361.

    CAS  Google Scholar 

  • Willmitzer, L., Schmalenbach, W., Schell, J., 1981: Transcription of T-DNA in octopine and nopaline crown gall tumours is inhibited by low concentrations of aamanitin. Nucl. Ac. Res. 9, 4801–4812.

    CAS  Google Scholar 

  • Willmitzer, L., Simons, G., Schell, J., 1982: The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts. EMBO J. 139–146.

    Google Scholar 

  • Willmitzer, L., Dhaese, P., Schreier, P. H., Schmalenbach, W., Van Montagu, M., Schell, J., 1983: Size, location and polarity of T-DNA encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors. Cell 32, 1045–1056.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Springer-Verlag/Wien

About this chapter

Cite this chapter

Hille, J., Hoekema, A., Hooykaas, P., Schilperoort, R. (1984). Gene Organization of the Ti-Plasmid. In: Verma, D.P.S., Hohn, T. (eds) Genes Involved in Microbe-Plant Interactions. Plant Gene Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8739-5_11

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-8739-5_11

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-8741-8

  • Online ISBN: 978-3-7091-8739-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics