Abstract
Plant chromatin, like animal chromatin, can be cleaved by micrococcal nuclease digestion into nucleosomal subunits. The DNA content of the nucleosome is 175 ± 8 base pairs in all plant species and tissues studied. The DNA content of the core particle is 140 base pairs. Compared to animal histones, plant histone fractions H 1, H 2A and H 2B differ with respect to their electrophoretic mobility on polyacrylamide-SDS gels. H 2A and H 2B are species specific. H 1 is species and tissue specific. Studies using chromatin from tissues of different physiological activity do not indicate a different chromatin structure for active and inactive chromatin. Ribosomal genes in active and inactive state seem to be arranged in a configuration which can be cleaved by micrococcal nuclease attack into the same subunit pattern as the bulk of chromatin.
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Leber, B., Hemleben, V. (1979). Nucleosomal Organization in Active and Inactive Plant Chromatin. In: Nagl, W., Hemleben, V., Ehrendorfer, F. (eds) Genome and Chromatin: Organization, Evolution, Function. Plant Systematics and Evolution, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8556-8_16
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DOI: https://doi.org/10.1007/978-3-7091-8556-8_16
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