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Application of Glass Capillary Gas Chromatography to the Study of Urinary Steroid Excretion in Normal Children and in Patients With Various Endocrinopathies

Conference paper
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Part of the Pädiatrie und Pädologie book series (PÄD SUPPL, volume 5)

Summary

A method of gas chromatography on glass capillary columns (g.c.c.c.) is presented which allows the determination of 26 urinary C19 and C21 steroid metabolites in one procedure.

Hundredthirtyseven normal individuals of both sexes from 6 months through 32 years of age were studied regarding their urinary steroid patterns. These were compared to the excretion patterns of patients with congenital adrenal hyperplasia before and during treatment and of a child with virilizing adrenal carcinoma. From the results it is concluded that g.c.c.c. may be considered a valuable tool in the study of steroid production and metabolism.

Abbreviations used in the text

Oe

estratetrienol (= internal standard)

Andro

androsterone

Et

etiocholanolone

DHA

dehydroepiandrosterone

11-O-Andro

11-ketoandrosterone

11-O-ET

11-ketoetiocholanolone

11-OH-Andro

11-hydroandrosterone

11-OH-ET

11-hydroxyetiocholanolone

PD

pregnanediol

TH-DOC

tetrahydrodesoxycorticosterone

PT

pregnanetriol

Δ5-PT

pregnenetriol

THA

tetrahydrodehydrocorticosterone

THB

tetrahydrocorticosterone

THS

tetrahydro-21-desoxycortisol

IS

cholesterylbutyrate (= internal standard)

THE

tetrahydrocortisone

THF

tetrahydrocortisol

allo-THF

allo-tetrahydrocortisol

Ch

cholesterol

MO-TMS

Methyloximtrimethysilyl

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References

  1. Gleispach, H.: Die Ausscheidung von 17-Oxosteroiden, Pregnanen und Testosteron im menschlichen Ham in Abhängigkeit von Alter und Geschlecht. Z. Klin. Chem. Biochem. 11, 482–486 (1973).Google Scholar
  2. Gleispach, H., Auer, B., Frisch, H., Karpellus, P.: Ein Beitrag zur Ausscheidung von Cortisol, Tetrahydrocortisol und Tetrahydrocortison im Ham von Knaben. Pädiat. u. Pädol. 9, 80–83 (1974a).Google Scholar
  3. Gleispach, H., Berger, H., Glatzl, J., Rössler, H.: Pregnantriolonausscheidung nach ACTH-Stimulierung als Test auf vermutliche heterozygote Erbmalsträger eines 21-Hydroxylasemangels. Pädiat. u. Pädol. 9, 204–208 (1974b).Google Scholar
  4. Homoki, J., Teller, W. M., Fazekas, A. T. A.: A Test for Heterozygocity of 21-Hydroxylase Deficiency. Hum. Genet. 32, 35–41 (1976).PubMedCrossRefGoogle Scholar
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Copyright information

© Springer-Verlag/Wien 1977

Authors and Affiliations

  1. 1.Department of PediatricsUniversity of UlmFederal Republic of Germany
  2. 2.Universitäts-KinderklinikUlmFederal Republic of Germany

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