Skip to main content
SpringerLink
Log in

Antibiotic Receptor-Sites in Escherichia coli Ribosomes

  • Conference paper
Book cover Drug Receptor Interactions in Antimicrobial Chemotherapy

Part of the book series: Topics in Infectious Diseases ((TIDIS,volume 1))

Abstract

The translation of mRNA into protein at the ribosomal level can be divided into three phases: initiation, elongation and termination. The initiation phase can be divided into: (a) Recognition of initiation factors and mRNA by the 30S subunit, (b) binding of f-Met-tRNAFet and (c) combination of this initiation complex with a 50S subunit.The elongation phase follows initiation of protein biosynthesis and is composed of repeated cycles. Each cycle can be divided into:(a) EF-Tu dependent aminoacyl-tRNA-binding, (b) peptide bond formation and (c) translocation. The translocation step requires the elongation factor G and GTP. The elongation cycle occurs repeatedly until the termination codon of the mRNA is reached. In response to this codon, release factors are bound and promote release of a newly synthesized protein from the tRNA moiety in a reaction catalyzed by the ribosomal peptidyltransferase (Haselkorn and Rothman-Denes, 1973; Pongs et al., 1974).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • BALLESTA, J.P.G. and D. Vazquez: Activities of ribosomal cores deprived of proteins L7, L10, L11 and L12. FEBS Letters 48, 266 (1974).

    Article  PubMed  CAS  Google Scholar 

  • BENJAMINI, E., D. Michaeli and J.D. Young: Antigenic determinants of proteins of defined sequences. In Current Topics in Microbiology and Immunology 58, 85 (1972).

    Chapter  Google Scholar 

  • BIRGE, E.A. and C.G. Kurland: Altered ribosomal protein in strepto-mycin-dependent Escherichia coli. Science 166, 1282 (1969).

    Article  PubMed  CAS  Google Scholar 

  • BIRGE, E.A. and C.G. Kurland: Reversion of a streptomycin-dependent strain of Escherichia coli. Mol. Gen. Genet. 109, 356 (1970).

    Article  PubMed  CAS  Google Scholar 

  • BISWAS, D.K. and L. Gorini: The attachment site of streptomycin to the 30S ribosomal subunit. Proc. Natl. Acad. Sci. USA 69, 2141 (1972).

    Article  PubMed  CAS  Google Scholar 

  • BJARE, U. and L. Gorini: Drug dependence reverted by ribosomal ambiguity mutation, ram. J. Mol. Biol.57, 423 (1971).

    Article  PubMed  CAS  Google Scholar 

  • BODE, U., L.C. Lutter and G. Stöffler: Proteins S14 and S19 are near- neigbors in the E. coli ribosome. FEBS Letters 45, 232 (1974).

    Article  PubMed  CAS  Google Scholar 

  • BOLLEN, A., T. Heiser, T. Yamada and J. Davies: Altered ribosomes in antibiotic-resistant mutants of E. coli. Cold Spring Harb. Symp. Quant. Biol. 34, 95 (1969).

    Article  CAS  Google Scholar 

  • BRECKENRIDGE, L. and L. Gorini: Genetic analysis of streptomycin resistance in Escherichia coli. Genetics 65, 9 (1970).

    PubMed  CAS  Google Scholar 

  • BUCKEL, P., D. Ruffler, W. Piepersberg and A Böck: RNA overproducing revertants of an alanyl-tRNA synthetase mutant of Escherichia coli. Mol. Gen. Genet. 119, 323 (1972).

    PubMed  CAS  Google Scholar 

  • BURNS, D.J.W. and E. Cundliffe: Bacterial-protein synthesis. A novel ystem for studying antibiotic action in vivo. Eur. J. Biochem. 37, 570 (1973).

    Article  PubMed  CAS  Google Scholar 

  • CANTOR, C.R.: Fluorescence spectroscopic approaches to the studies of three-dimensional structure of ribosomes. In Ribosomes, Nomura, Tissières and Lengyel, Edts.; Cold Spring Harbor, New York (1974).

    Google Scholar 

  • COX, E.C., J.R. White and J.G. Flaks: Streptomycin action and the ribosome. Proc. Natl. Acad. Sci. USA 51, 703 (1964).

    Article  PubMed  CAS  Google Scholar 

  • DAVIES, J.E.: Studies on the ribosomes of streptomycin-sensitive and resistant strains of Escherichia coli. Proc. Natl. Acad. Sci. USA 51, 659 (1 964).

    Google Scholar 

  • DAMES, J., W. Gilbert and L. Gorini: Streptomycin, suppression, and the code. Proc. Natl. Acad. Sci. USA 51, 883 (1964).

    Article  Google Scholar 

  • DAVIES, J., L. Gorini and B.D. Davis: Misreading of RNA codewords induced by aminoglycoside antibiotics. Mol. Pharmacol. 1, 93 (1965).

    PubMed  CAS  Google Scholar 

  • DEKIO, S. and R. Takata: Genetic studies of the ribosomal proteins in Escherichia coli II. Altered 30S ribosomal protein component specific to spectinomycin-resistant mutants. Mol. Gen. Genet. 105, 219 (1969).

    Article  PubMed  CAS  Google Scholar 

  • DEUSSER, E., G. Stöffler, H.G. Wittmann and D. Apirion: Ribosomal Proteins XVI, Altered S4 proteins in Escherichia coli revertants from streptomycin dependence to independence. Mol. Gen. Genet. 109, 298 (1970).

    Article  PubMed  CAS  Google Scholar 

  • DE WILDE, M. and B. Wittmann-Liebold: Localization of the amino acid exchange in protein S5 from Escherichia coli mutant resistant to spectinomycin. Mol. Gen. Genet. 127, 273 (1973).

    Article  Google Scholar 

  • DONNER, D. and C.G. Kurland: Changes in the primary structure of a mutationally altered ribosomal protein. Mol. Gen. Genet. 115, 49 (1972).

    Article  PubMed  CAS  Google Scholar 

  • DUBNAU, D., C. Goldthwaite, I. Smith and J. Murmur: Genetic mapping in Bacillus subtilis. J. Mol. Biol. 27, 163 (1967).

    Article  PubMed  CAS  Google Scholar 

  • ERDÖS, T. and A. Ullmann: Effect of streptomycin on the incorporation of amino acids labelled with carbon-14 into ribonucleic acid and protein in a cell-free system of a mycobacterium. Nature 183, 618 (1959).

    Article  PubMed  Google Scholar 

  • FELLNER, P.: Structure of the 16S and 23S ribosomal RNAs. In Ribosomes, Nomura, Tissières and Lengyel, Edts.; Cold Spring Harbor, New York (1974).

    Google Scholar 

  • FITZGERALD, R.J., F. Bernheim and D.B. Fitzgerald: The inhibition by streptomycin of adaptive enzyme formation in mycobacteria. J. Biol. Chem. 175, 195 (1948).

    PubMed  CAS  Google Scholar 

  • FLAKS, J.G., E.C. Cox, M.L. Witting and J.R. White: Polypeptide synthesis with ribosomes from streptomycin-resistant and dependent Escherichia coli. Biochim. Biophys. Res 7, 390 (1962).

    Article  CAS  Google Scholar 

  • FUNATSU, G., K.H. Nierhaus and H.G. Wittmann: Determination of allele types and amino acid exchange in protein S12 of three streptomycin resistant mutants of Escherichia coli. Biochim. Biophys. Acta 287, 282 (1972).

    PubMed  CAS  Google Scholar 

  • FUNATSU, G., K. Nierhaus and B. Wittmann-Liebold: Ribosomal Proteins XXII. Studies on the altered protein S5 from a spectinomycinresistant mutant of Escherichia coli. J. Mol. Biol. 64, 201 (1972).

    Article  PubMed  CAS  Google Scholar 

  • FUNATSU, G., W. Puls, E. Schiltz, J. Reinbolt and H.G. Wittmann: Ribosomal Proteins XXXI. Comparativestudies on altered proteins S4 of six Escherichia coli revertants from streptomycin dependence. Mol. Gen. Genet. 115, 131 (1972).

    Article  PubMed  CAS  Google Scholar 

  • FUNATSU, G., E. Schiltz and H.G. Wittmann: Ribosomal Proteins XXVII. Localization of the amino acid exchanges in protein S5 from two E. coli mutants resistant to spectinomycin. Mol. Gen. Genet. 114, 106 (1971).

    Article  Google Scholar 

  • FUNATSU, G. and H.G. Wittmann: Ribosomal Proteins XXXIII. Location of amino acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin. J. Mol. Biol. 68, 547 (1972).

    Article  PubMed  CAS  Google Scholar 

  • GARRETT, R.A. and H.G. Wittmann: Protein-RNA interaction in bacterial ribosomes. In Protein Synthesis in Reproductive Tissue, Diczfalusy, Ed.; Karolinska Symposia on Research Methods in Reproductive Endocrinology, 6th Symp., Stockholm, 1973, P. 75.

    Google Scholar 

  • GRAY, P.N., G. Bellemare, R. Monier, R.A. Garrett and G. Stöffler: Identification of the nucleotide sequences involved in the interaction between Escherichia coli 5S RNA and specific 50S subunit proteins. J. Mol. Biol. 77, 133 (1973).

    Article  PubMed  CAS  Google Scholar 

  • GRAY, P.N., R.A. Garrett, G -Stöffler and R. Monier: An attempt at the identification of the proteins involved in the incorporation of 5-S RNA during 50-S ribosomal subunit assembly. Eur. J. Biochem. 28, 412 (1972).

    Article  PubMed  CAS  Google Scholar 

  • GOLDTHWAITE, C. and I. Smith: Genetic mapping of aminoglycoside and fusidic acid resistant mutations in Bacillus subtilis. Mol. Gen. Genet. 114, 181 (1972).

    Article  PubMed  CAS  Google Scholar 

  • GORINI, L. and E. Kataja: Phenotypic repair by streptomycin of defect-ive genotypes in E. coli. Proc. Natl. Acad. Sci. USA 51, 487 (1964).

    Article  PubMed  CAS  Google Scholar 

  • GUTHRIE, C., H. Nashimoto and M. Nomura: Studies on the assembly of ribosomes in vivo. Cold Spring Harb. Symp. Quant. Biol. 34, 69 (1969).

    Article  CAS  Google Scholar 

  • HAHN, F.E. and J. Ciak: Studies on the mode of action of streptomycin. I. Inhibition of bacterial protein synthesis by streptomycin.Bact. Proc. 131 (1959).

    Google Scholar 

  • HAHN, F.E. and C.L. Wisseman: Inhibition of adaptive enzyme formation by antimicrobial agents. Proc. Soc. Exptl. Biol. Med. 76, 533 (1951).

    CAS  Google Scholar 

  • HAMEL, E., M. Koka and T. Nakamoto: Requirement of an E. coli 50S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate. J. Biol. Chem. 247, 805 (1972).

    PubMed  CAS  Google Scholar 

  • HASELKORN, R. and L.B. Rothman-Denes: Protein Synthesis. Ann. Rev. Biochem. 42, 397 (1973).

    Article  PubMed  CAS  Google Scholar 

  • HASENBANK, R., C. Guthrie, G. Stöffler, H.G. Wittmann, L. Rosen and D. Apirion: Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Mol. Gen. Genet. 127, 1 (1973).

    Article  PubMed  CAS  Google Scholar 

  • HASHIMOTO, K.: Streptomycin resistance in Escherichia coli analyzed by transduction. Genetics 45, 49 (1960).

    PubMed  CAS  Google Scholar 

  • HELSER, T.L., J.E. Davies and J.E. Dahlberg: Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli. Nature New Biology 233, 12 (1971).

    PubMed  CAS  Google Scholar 

  • HELSER, T.L., J.E. Davies and J.E. Dahlberg: Mechanism of kasugamycin resistance in Escherichia coli. Nature New Biology 235, 6 (1972).

    PubMed  CAS  Google Scholar 

  • HIGHLAND, J.H., J.WI Bodley, J. Gordon, R. Hasenbank and G. Stöffler: Identity of the ribosomal proteins involved in the interaction with elongation factor G. Proc. Natl. Acad. Sci. USA 70, 142 (1973).

    Article  Google Scholar 

  • HIGHLAND, J.H., J.WI Bodley, J. Gordon, R. Hasenbank and G. Stöffler: Identity of the ribosomal proteins involved in the interaction with elongation factor G. Proc. Natl. Acad. Sci. USA 70, 142 (1973).

    Article  Google Scholar 

  • HIGHLAND, J.H., E. Ochsner, J. Gordon, J.W. Bodley, R. Hasenbank and G. Stöffler: Coordinate inhibition of elongation factor G function and ribosomal subunit association by antibodies to several ribosomal proteins. Proc. Natl. Acad. Sci. USA 71, 627 (1974).

    Article  PubMed  CAS  Google Scholar 

  • HORNE, J.R. and V.A. Erdmann: Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophiZus and Escherichia coli ribosomes. Mol. Gen. Genet. 119, 337 (1972).

    PubMed  CAS  Google Scholar 

  • HORNE, J.R. and V.A. Erdmann: ATPase and GTPase activities associated with a specific 5S RNA-protein complex. Proc. Natl. Acad. Sci. USA 70, 2870 (1973).

    Article  PubMed  CAS  Google Scholar 

  • HORNE, J.R. and V.A. Erdmann: Effects of ethanol, methanol and different antibiotics on the ATPase and GTPase activities associated with B. stearothermophiZus 5S RNS-protein complex. FEBS Letters 42, 42 (1974).

    Article  PubMed  CAS  Google Scholar 

  • HOWARD, G.A. and J. Gordon: Peptidyltransferase activity of ribosomal particles lacking protein L11. FEBS Letters 48, 271 (1974).

    Article  PubMed  CAS  Google Scholar 

  • HSIUNG, N., S.A. Reines and C.R. Cantor: Investigation of the ribosomal peptidyl transferase center using a photoaffinity label. J. Mol. Biol. 88, 841 (1974).

    Article  PubMed  CAS  Google Scholar 

  • HUANG, K.H. and C.R. Cantor: Surface topography of the 30S Escherichic coli ribosomal subunit: Reactivity towards fluorescein isothiocyanate. J. Mol. Biol. 67, 265 (1972).

    Article  PubMed  CAS  Google Scholar 

  • ITOH, T. and H.G. Wittmann Amino acid replacement in protein S5 and S12 from streptomycin dependence to independence. Mol. Gen. Genet. 127, 19 (1973).

    Article  CAS  Google Scholar 

  • KAJI, H. and Y. Tanaka: Binding of dihydrostreptomycin to ribosomal subunits. J. Mol. Biol. 32, 221 (1968).

    Article  PubMed  CAS  Google Scholar 

  • KALTSCHMIDT, E. and H.G. Wittmann: Ribosomal proteins XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc. Natl. Acad. Sci. USA 67, 1276 (1970).

    Article  PubMed  CAS  Google Scholar 

  • KISCHA, K., W. Möller and G. Stöffler: Reconstitution of a GTPase activity by a 50S ribosomal protein from E. coli. Nature 233, 62 (1971).

    Article  CAS  Google Scholar 

  • KREIDER, G. and B.L. Brownstein: A mutation suppressing streptomycin dependence. II. An altered protein in the 30S ribosomal subunit. J. Mol. Biol. 61, 135 (1971).

    Article  PubMed  CAS  Google Scholar 

  • KREIDER, G. and B.L. Brownstein: Ribosomal proteins involved in the suppression of streptomycin dependence in Escherichia coli. J. Bact. 109, 780 (1972).

    PubMed  CAS  Google Scholar 

  • KURLAND, C.G Functional organization of the 30S ribosomal subunit. In Ribosomes, Nomura, Tissières and Lengyel, Edts.; Cold Spring Harbor, New York (1974).

    Google Scholar 

  • LELONG, J.C., H.A.Cousin and F.Gros, R. Miskin, Z. Vogel, Y. Groner and M. Revel: Protection of Escherichia coli ribosomes against streptomycin by purified initiation factors. Eur. J. Biochem. 27, 174 (1972).

    Article  PubMed  CAS  Google Scholar 

  • LELONG, J.C., D. Gros, F. Gros, A. Bollen, R. Maschler and G. Stöffler: Function of individual 30S subunit proteins of E. Coli. The effect of specific immunoglobulin fragments (Fab) on the activities of ribosomal decoding sites. Proc. Natl. Acad. Sci. USA 71, 248 (1974).

    Article  PubMed  CAS  Google Scholar 

  • LUTTER, L.C., U. Bode, C.G. Kurland and G. Stöffler: Ribosomal protein neighborhoods III. Cooperativity of assembly. Mol. Gen. Genet. 129, 167 (1974).

    Article  PubMed  CAS  Google Scholar 

  • LUTTER, L.C., H. Zeichhardt, C.G. Kurland and G. Stöffler: Ribosomal protein neighborhoods I. S18 and S21 as well as S5 and S8 are neighbors. Mol. Gen. Genet. 119, 357 (1972).

    PubMed  CAS  Google Scholar 

  • MASUKAWA, H.: Localization of sensitivity to kanamycin and streptomycin in 30S ribosomal proteins of Escherichia coli. J. Antibiotics 22, 612 (1969).

    CAS  Google Scholar 

  • MOLLER, W.: The ribosomal components involved in EF-G-and EF-Tudependent GTP hydrolysis. In Ribosomes, Nomura, Tissières and Lengyel, Edts., Cold Spring Harbor, New York (1974).

    Google Scholar 

  • MOMOSE, H. and L. Gorini: Genetic analysis of streptomycin dependence in Escherichia coli. Genetics 67, 19 (1971).

    PubMed  CAS  Google Scholar 

  • MONIER, R.: 5S RNA. In Ribosomes, Nomura, Tissières and Lengyel, Edts., Cold Spring Harbor, New York (1974).

    Google Scholar 

  • MORGAN, J. and R. Brimacombe: A preliminary three-dimensional arrangement of the proteins in the Escherichia coli 30S ribosomal sub-particle. Eur. J. Biochem. 37, 472 (1973).

    Article  PubMed  CAS  Google Scholar 

  • MORRISON, C.A., R.A. Garrett, H. Zeichhardt and G. Stöffler: Proteins occurring at, or near the subunit interface of E. coli ribosomes. Mol. Gen. Genet. 127, 359 (1973).

    Article  PubMed  CAS  Google Scholar 

  • NASHIMOTO, H., W. Held, E. Kaltschmidt and M. Nomura: Structure and function of bacterial ribosomes. XII. Accumulation of 21S particles by some cold-sensitive mutants of Escherichia coli. J. Mol. Biol. 62, 121 (1971).

    Article  PubMed  CAS  Google Scholar 

  • NASHIMOTO, H. and M. Nomura: Structure and function of bacterial ribosomes XI. Dependence of 50S ribosomal assembly on simultaneous assembly of 30S subunits. Proc. Natl. Acad. Sci. USA 67, 1440 (1970).

    Google Scholar 

  • NEWCOMBE, H.B. and M.H. Nyholm: The inheritance of streptomycin resistance and dependence in crosses of Escherichia. coli. Genetics 35, 603 (1950).

    PubMed  CAS  Google Scholar 

  • NIERHAUS, K.H. and O. Montejo: A protein involved in the peptidyltransferase activity of Escherichia coli ribosomes. Proc. Natl. Acad. Sci. USA 70, 1931 (1973).

    Article  PubMed  CAS  Google Scholar 

  • NIERHAUS, D. and K.H. Nierhaus: Identification of the chloramphenicolbinding protein in Escherichia coli ribosomes by partial reconstitution. Proc. Natl. Acad. Sci. USA 70, 2224 (1973).

    Article  PubMed  CAS  Google Scholar 

  • NOMURA, M. and V.A. Erdmann: Reconstitution of 50S ribosomal subunits from dissociated molecular components. Nature 228, 744 (1970).

    Article  PubMed  CAS  Google Scholar 

  • NOMURA, M., S. Mizushima, M. Ozaki, P. Traub and C.V. Lowry: Structure and function of ribosomes and their molecular components. Cold Spring Harb. Symp. Quant. Biol. 34, 49 (1969).

    Article  CAS  Google Scholar 

  • OSAWA, S., E. Otake, R. Takata, S. Dekio, M. Matsubara, T. Itoh and A. Muto: Ribosomal protein genes in bacteria. FEBS Symp. 23, 313 (1972).

    Google Scholar 

  • OSAWA, S., R. Takata, K. Tanaka and M. Tamaki: Chloramphenicol resistant mutants of Bacillus subtilis. Mol. Gen. Genet. 127, 163 (1973).

    Article  PubMed  CAS  Google Scholar 

  • OTAKA, E., T. Itoh and S. Osawa: Ribosomal proteins of bacterial cells: Strain-and species-specificity. J. Mol. Biol. 33, 93 (1968).

    Article  PubMed  CAS  Google Scholar 

  • OTAKA, E., T. Itoh, S. Osawa, K. Tanaka and M. Tamaki: Peptide analyses of a protein component, 50–8, of 50S ribosomal subunits from erythromycin resistant mutants of Escherichia coli and Escherichia freundii. Mol. Gen. Genet. 114, 14 (1971).

    Article  Google Scholar 

  • OZAKI, M., S. Mizushima and M. Nomura: Identification and functional characterization of the protein controlled by the streptomycin resistant locus in E. coli. Nature 222, 333 (1969).

    Article  PubMed  CAS  Google Scholar 

  • PESTKA, S.: Inhibitors of ribosome functions. Ann. Rev. Microbiol. 25, 487 (1971).

    Article  CAS  Google Scholar 

  • PESTKA, S. and J.W. Bodley: In Antibiotics, Gottlieb and Shaw, Edts., Springer, Berlin-Heidelberg-New York (1974).

    Google Scholar 

  • PONGS, 0., R. Bald and V.A. Erdmann: Identification of chloramphenicol-binding protein in Escherichia coli ribosomes by affinity labeling. Proc. Natl. Acad. Sci. USA 70, 2229 (1973).

    Article  Google Scholar 

  • PONGS, 0. and V.A. Erdmann: Affinity labeling of E. coli ribosomes with a streptomycin-analogue. FEBS Letters 37, 47 (1973).

    Article  Google Scholar 

  • PONGS, 0., K.H. Nierhaus, V.A. Erdmann and H.G. Wittmann: Active sites in Escherichia coli ribosomes. FEBS Letters 40, S28 (1974).

    Article  Google Scholar 

  • REINBOLT, J. and E. Schiltz: The primary structure of ribosomal protein S4 from Escherichia coli. FEBS Letters 36, 250 (1973).

    Article  PubMed  CAS  Google Scholar 

  • ROSSET, R. and L. Gorini: Ribosomal ambiguity mutation. J. Mol. Biol. 39, 95 (1969).

    Article  PubMed  CAS  Google Scholar 

  • SCHREINER, G. and K.H. Nierhaus: Protein involved in the binding of dihydrostreptomycin to ribosomes of Escherichia coli. J. Mol. Biol. 81, 71 (1973).

    Article  PubMed  CAS  Google Scholar 

  • SMITH, I., C. Goldthwaite and D. Dubnau: The genetic of ribosomes in Bacillus subtilis. Cold Spring Harb. Symp. Quant. Biol. 34, 85 (1969).

    Article  CAS  Google Scholar 

  • SPEYER, J.F., P. Lengyel and V. Basilio: Ribosomal localization of streptomycin sensitivity. Proc. Natl. Acad. Sci. USA 48, 684 (1962).

    Article  PubMed  CAS  Google Scholar 

  • SPOTTS, C.R. and R.Y. Stanier: Mechanism of streptomycin action on bacteria: A unitary hypothesis. Nature 192, 633 (1961).

    Article  PubMed  CAS  Google Scholar 

  • STAEHELIN, T., D. Maglott and R.E. Monro: On the catalytic center of peptidyl transfer: A part of the 50S ribosome structure. Cold Spring Harb. Symp. Quant. Biol. 34, 39 (1969).

    Article  CAS  Google Scholar 

  • STAEHELIN, T. and M. Meselson: Determination of streptomycin sensi- tivity by a subunit of the 30S ribosome of Escherichia coli. J. Mol. Biol. 19, 207 (1966).

    Article  PubMed  CAS  Google Scholar 

  • STÖFFLER, G.: Structure and function of the Escherichia coli ribosome: Immunological analysis. In Ribosomes, Nomura, Tissières and Lengyel, Edts., Cold Spring Harbor, New York (1974).

    Google Scholar 

  • STÖFFLER, G., E. Deusser, H.G. Wittmann and D. Apirion: Ribosomal Proteins XIX. Altered S5 ribosomal protein in an Escherichia coli revertant from streptomycin dependence to independence. Mol. Gen. Genet. 111, 334 (1971).

    Article  PubMed  Google Scholar 

  • STÖFFLER, G., R. Hasenbank, M. Lütgehaus, R. Maschler, C.A. Morrison, H. Zeichhardt and R.A. Garrett: The accessibility of proteins of the Escherichia coli ribosomal subunit to antibody binding. Mol. Gen. Genet. 127, 89 (1973).

    Article  PubMed  Google Scholar 

  • STÖFFLER, G. and H.G. Wittmann: Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods. Proc. Natl. Acad. Sci. USA 68, 2283 (1971a).

    Article  PubMed  Google Scholar 

  • STÖFFLER, G. and H.G. Wittmann Ribosomal Proteins, XXV. Immunological studies on Escherichia coli ribosomal proteins. J. Mol. Biol. 62, 407 (1971b).

    Article  PubMed  Google Scholar 

  • SZEKELY, M., R. Brimacombe and J. Morgan: A specific ribonucleoprotein fragment from Escherichia coli 30S ribosomes. Location of the RNA component in 16S RNA. Eur. J. Biochem. 35, 574 (1973).

    Article  PubMed  CAS  Google Scholar 

  • TANAKA, N., H. Masukawa and U. Umezawa: Structural basis of kanamycin for miscoding activity. Biochem. Biophys. Res. 26, 544 (1967).

    Article  CAS  Google Scholar 

  • TANAKA, K., M. Tamaki, A. Kimura, R. Takata and S. Osawa: Erythromycin resistant mutants from Bacillus subtilis. Mol. Gen. Genet. 127, 157 (1973).

    Article  PubMed  CAS  Google Scholar 

  • TANAKA, K., H. Teraoka, M. Tamaki, R. Takata and S. Osawa: Phenotypes represented by a mutational change in a 50S ribosomal protein component, 50–8, in Escherichia coli. Mol. Gen. Genet. 114, 9 (1971).

    Google Scholar 

  • TANAKA, K., H. Teraoka, M. Tamaki, R. Takata and S. Osawa: Phenotypes represented by a mutational change in a 50S ribosomal protein component, 50–8, in Escherichia coli. Mol. Gen. Genet. 114, 9 (1971).

    Google Scholar 

  • TISCHENDORF, G.W., H. Zeichhardt and G. Stöffler: Location of proteins S5, S13 and S14 on the surface of the 30S ribosomal subunit from Escherichia coli as determined by immune electron microscopy. Mol. Gen. Genet. 134, 209 (1974a).

    Article  PubMed  CAS  Google Scholar 

  • TISCHENDORF, G.W., H. Zeichhardt and G. Stöffler: Determination of the location of proteins L14, L17, L18, L19, L22 and L23 on the surface of the 50S ribosomal subunit of Escherichia coli by immune electron microscopy. Mol. Gen. Genet. 134, 187 (1974).

    Article  PubMed  CAS  Google Scholar 

  • TRAUB, P., K. Hosokawa and M. Nomura: Streptomycin sensitivity and the structural components of the 30S ribosomes of Escherichia coli. J. Mol. Biol. 19, 211 (1966).

    Article  PubMed  CAS  Google Scholar 

  • TRAUB, P. and Nomura, M.: Structure and function of E coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and protein. Proc. Natl. Acad. Sci. USA 59, 777 (1968).

    CAS  Google Scholar 

  • TRAUT, R.R.: Protein topography by ribosomal subunits from Escherichia coli. In Ribosomes, Nomura, Tissières and Lengyel, Edts., Cold Spring Harbor, New York (1974).

    Google Scholar 

  • VAZQUEZ, D.: Inhibitors of protein synthesis. FEBS Letters 40, S63 (1974).

    Article  PubMed  Google Scholar 

  • WABL, M.R.: Electron microscopic localization of two proteins on the surface of the 50S ribosomal subunit of Escherichia coli using specific antibody markers. J. Mol. Biol. 84, 241 (1974).

    Article  PubMed  CAS  Google Scholar 

  • WEBER, H.J.: Stoichiometric measurements of 30S and 50S ribosomal proteins from Escherichia coli. Mol. Gen. Genet. 119, 233 (1972).

    Article  PubMed  CAS  Google Scholar 

  • WEISBLUM, B. and J. Davies: Antibiotic inhibitors of the bacterial ribosome. Bacteriol. Rev. 32, 493 (1968).

    PubMed  CAS  Google Scholar 

  • WITTMANN, H.G.: Purification and identification of Escherichia coli ribosomal proteins. In Ribosomes, Nomura, Tissières and Lengyel, Edts., Cold Spring Harbor, New York (1974).

    Google Scholar 

  • WITTMANN-LIEBOLD, B. and H.G. Wittmann: Ribosomal Proteins XX. Isolation and analysis of the tryptic peptides of proteins S5 from strain K and B of Escherichia coli. Biochim. Biophys. Acta 251, 44 (1971).

    Google Scholar 

  • WITTMANN, H.G., G. Stöffler, D. Apirion, L. Rosen, K. Tanaka, M. Tamaki, R. Takata, S. Dekio, E. Otake and S. Osawa: Biochemical and genetic studies on two different types of erythromycin re-sistant mutants of Escherichia coli with altered ribosomal proteins. Mol. Gen. Genet. 127, 175 (1973).

    Article  PubMed  CAS  Google Scholar 

  • WITTMANN, H.G. and B. Wittmann-Liebold: Chemical structure of bacterial ribosomal proteins. In Ribosomes, Nomura, Tissières and Lengyel, Edts., Cold Spring Harbor, New York (1974).

    Google Scholar 

  • ZIMMERMANN, R.A. and Feltner, P.: RNA-protein interactions in the ribosome. In Ribosomes, Nomura, Tissières and Lengyel, Edts.,Cold Spring Harbor, New York (1974).

    Google Scholar 

  • ZIMMERMANN, R.A., R.T. Garvin and L. Gorini: Alteration of a 30S ribosomal protein accompanying ram mutation in Escherichia coli Proc. Natl. Acad. Sci. USA 68, 2263 (1971).

    Article  CAS  Google Scholar 

  • ZIMMERMANN, R.A., Y. Ikeya and P.F. Sparling: Alteration of ribosomal protein S4 by mutation linked to kasugamycin resistance in Escherichia coli. Proc. Natl. Acad. Sci. USA 70, 71 (1973).

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Georg Stöffler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gilbert W. Tischendorf
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Sandoz Forschungsinstitut Gesellschaft m. b. H., Vienna, Austria

    Jürgen Drews

  2. Walter Reed Army Medical Center, Washington, D.C., USA

    E. Hahn Ph. D.

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer-Verlag/Wien

About this paper

Cite this paper

Stöffler, G., Tischendorf, G. (1975). Antibiotic Receptor-Sites in Escherichia coli Ribosomes. In: Drews, J., Hahn, E. (eds) Drug Receptor Interactions in Antimicrobial Chemotherapy. Topics in Infectious Diseases, vol 1. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8405-9_9

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-8405-9_9

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-8407-3

  • Online ISBN: 978-3-7091-8405-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation