Skip to main content

Experiments on the Binding Sites and the Action of Some Antibiotics which Inhibit Ribosomal Functions

  • Conference paper
Book cover Drug Receptor Interactions in Antimicrobial Chemotherapy

Part of the book series: Topics in Infectious Diseases ((TIDIS,volume 1))

Abstract

We describe experiments (A) on an antibiotic which binds to the 50S subunit of procaryotic ribosomes (chloramphenicol), (B) on an antibiotic which binds specifically to the 30S subunit (streptomycin), and (C) on a group of antibiotics whose binding sites are not yet well known (tetracycline and some of its derivatives).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • BISWAS, D.K., and L. GORINI: The attachment site of streptomycin to the 30S ribosomal subunit. Proc. Nat. Acad. Sci. USA 69, 2141–2144 (1972).

    Article  PubMed  CAS  Google Scholar 

  • BODLEY, J.N., and F.J. ZIEVE: On the specificity of the two ribosomal binding sites: studies with tetracycline. Biochem. Biophys. Res. Commun. 36, 463–468 (1969).

    Article  PubMed  CAS  Google Scholar 

  • CAPECCHI, M.R., and H.A. KLEIN: Characterization of three proteins involved in polypeptide chain termination. Cold Spring Harbor Symp. Quant. Biol. 34, 469–491 (1969).

    CAS  Google Scholar 

  • CELMA, M.L., R.F. MONRO, and D. VAZQUEZ: Substrate and antibiotic binding sites at the peptidyltransferase center of E. coli ribosomes: Binding of UACCA-Leu to 50S subunits. FEBS Lett. 13, 247251 (1971).

    Google Scholar 

  • CERNA, J., I. RYCHLIK, and P. PULKRABEK: The effect of antibiotics on the coded binding of peptidyl-tRNA to the ribosome and on the transfer of the peptidylresidue to puromycin. Eur. J. Biochem. 9, 27–35 (1969).

    Article  PubMed  CAS  Google Scholar 

  • DAY, L.E.: Tetracycline inhibition of cell-free protein synthesis. J. Bacteriol. 92, 197–203 (1966).

    PubMed  CAS  Google Scholar 

  • DIETRICH, S., I. SCHRANDT, and K.H. NIERHAUS: Interdependence of E. coli ribosomal proteins at the peptidyltransferase center. FEBS Letters, in press.

    Google Scholar 

  • FERNANDEZ-MUNOZ, R., R.E. MONRO, R. TORRES-PINEDO, and D. VAZQUEZ: Substrate-and antibiotic binding sites of the peptidyltransferase center of Escherichia coli ribosomes. Eur. J. Biochem. 23, 185–193 (1971a).

    Article  Google Scholar 

  • FERNANDEZ-MUNOZ, R., R.E. MONRO, and D. VAZQUEZ: Ribosomal peptidyltransferase: Binding of inhibitors. Methods in Enzymology (Academic Press, New York and London), Vol. XX, part C, 481–490 (1971b).

    Google Scholar 

  • GOTTESMAN, M.E.: Reaction of ribosome-bound peptidyl transfer ribonucleic acid or puromycin. J. Biol. Chem. 242, 5564–5571 (1967).

    PubMed  CAS  Google Scholar 

  • HIEROWSKI, M.: Inhibition of protein synthesis by chlortetracycline in the Escherichia coli in vitro system. Proc. Nat. Acad. Sci. USA 53, 594–599 (1965).

    Article  PubMed  CAS  Google Scholar 

  • HOMANN, H.E., and K.H. NIERHAUS: Protein compositions of biosynthetic precursors and artificial subparticles from ribosomal subunits in Escherichia coli K12. Eur. J. Biochem. 20, 249–257 (1971).

    Article  PubMed  CAS  Google Scholar 

  • LESSARD, J.L., and PESTKA, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. CCIII. Chloramphenicol, aminoacyl oligonucleotides, and Escherichia coli ribosomes. J. Biol. Chem. 247, 6909–6912 (1972).

    PubMed  CAS  Google Scholar 

  • MAXWELL, J.H.: Studies of the binding of tetracycline to ribosomes in vitro. Mol. Pharmacol. 4, 25–37 (1968).

    PubMed  CAS  Google Scholar 

  • MIZUSHIMA, S., and M. NOMURA: Assembly mapping of 30S ribosomal proteins from E. coli. Nature (London) 226, 1214–1218 (1970).

    Article  CAS  Google Scholar 

  • NIERHAUS, K.H., and V. MONTEJO: A protein involved in the peptidyltransferase activity of Escherichia coli ribosomes. Proc. Nat. Acad. Sci. USA 70, 1931–1935 (1973).

    Article  PubMed  CAS  Google Scholar 

  • NIERHAUS, D., and K.H. NIERHAUS: Identification of the chloramphenicol-binding protein in Escherichia coli ribosomes by partial reconstitution. Proc. Nat. Acad. Sci. USA 70, 2224–2228 (1973).

    Article  PubMed  CAS  Google Scholar 

  • PESTKA, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenyl alanyl-oligonucleotide binding to ribosomes. Proc. Nat. Acad. Sci. USA 64, 709–714 (1969).

    Article  PubMed  CAS  Google Scholar 

  • ROTH, H.E., and K.H. NIERHAUS: Isolation of four ribonucleoprotein fragments from the 30S subunit of E. coli ribosomes. FEES Lett. 31, 35–38 (1973).

    Article  CAS  Google Scholar 

  • SCHREINER, G., and K.H. NIERHAUS: Protein involved in the binding of dihydrostreptomycin to ribosomes of Escherichia coli. J. Mol. Biol. 81, 71–82 (1973).

    Article  PubMed  CAS  Google Scholar 

  • SUAREZ, G., and D. NATHANS: Inhibition of aminoacyl-sRNA binding to ribosomes by tetracycline. Biochem. Biophys. Res. Commun. 18, 743–750 (1965).

    Article  CAS  Google Scholar 

  • VOGEL, Z., A. ZAMIR, and D. ELSON: The possible involvement of peptidyltransferase in the termination step of protein biosynthesis. Biochemistry 8, 5161 (1969).

    Article  PubMed  CAS  Google Scholar 

  • WATANABE, S.: Interaction of siomycin with the acceptor site of Escherichia coli ribosomes. J. Mol. Biol. 67, 443–457 (1972).

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer-Verlag/Wien

About this paper

Cite this paper

Werner, R., Kollak, A., Nierhaus, D., Schreiner, G., Nierhaus, K.H. (1975). Experiments on the Binding Sites and the Action of Some Antibiotics which Inhibit Ribosomal Functions. In: Drews, J., Hahn, E. (eds) Drug Receptor Interactions in Antimicrobial Chemotherapy. Topics in Infectious Diseases, vol 1. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8405-9_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-8405-9_15

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-8407-3

  • Online ISBN: 978-3-7091-8405-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics