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Die Anwendung von in vitro-Embryotoxizitätstests in der pharmazeutisch-chemischen Industrie

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Forschung ohne Tierversuche 1996

Part of the book series: Ersatz- und Ergänzungsmethoden zu Tierversuchen ((TIERVERSUCHE))

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Literatur

  • Abir R., Ornoy A., Hur H.B., Jaffe P., Pinus H., IgG exchange as a means of partial correction of anomalies in rat embryos in vitro, induced by sera from women with recurrent abortion, Toxic in Vitro, 7, 817–826, 1993

    Article  CAS  Google Scholar 

  • Barber C.V., Carda M.B., Fantel A.G., A new technique for culturing rat embryos between gestation days 14 and 15, Toxic in Vitro, 7, 695–700, 1993

    Article  CAS  Google Scholar 

  • Bartmann, K., KRafft N., Bechterr., Rat Whole Embryo Culture Assay: 15 years of experience in pharmaceutical industry, Teratology Society, 24.-29. June, Newport Beach, Teratology, 51, 175, 1995

    Google Scholar 

  • BEchterr., The validation and use of in vitro teratogenicity tests, Arch Toxicol Suppl., 17, 170–191, 1995

    PubMed  CAS  Google Scholar 

  • BEchter, R. and SChmid, B.P., Teratogenicity in vitro - a comparative study of four antimycotic drugs using the whole-embryo culture system, Toxic in Vitro. 1, 11–15, 1987

    Article  CAS  Google Scholar 

  • BEchter, R. and BRouillard J.F., The effects of different chemical forms of a test compound on embiyotoxicity, distribution and metabolism in vitro, Toxic in Vitro, 2, 181–188, 1988

    Article  CAS  Google Scholar 

  • BEchter, R., BOuis, P., FIscher, V., Primary hepatocyte culture as an activating system for xenobiotica tested in the rat whole embryo in vitro, in: GOldberg A.M. and PRincipe M.L. (eds.), Alternative methods in toxicology - in vitro toxicology new directions, New York: Mary Ann Liebert Inc, vol 7, 313–326, 1989

    Google Scholar 

  • BEchter R., TErlouw G.D.C., LEe Q.P., JUchau M.R., Effects of QA 208–199 and its metabolite 209–668 on embryonic development in vitro after microinjection into the exocoelomic space or into the amniotic cavity of cultured rat conceptuses, Teratogenesis, Carcinogenesis and Mutagenesis, 11, 185–194, 1991

    Article  CAS  Google Scholar 

  • BEchter R., TErlouw G.D.C., TSuchiya M., TSuchiya T., KIstler A., Teratogenicity of arotinoids (retinoids) in the rat whole embryo culture, Arch Toxicol, 66, 193–197, 1992

    Article  PubMed  CAS  Google Scholar 

  • BEchter R., KRafft N., BArtmann K., The rat limb bud cell culture assay, Proceedings of the Symposium on methods in developmental toxicology/biology, Berlin, May 31–June 2, 1995, in press

    Google Scholar 

  • BRown N.A. and FAbro S., Quantitation of rat embryonic development in vitro: a morphological scoring system, Teratology, 24, 65–78, 1981

    Article  PubMed  CAS  Google Scholar 

  • CHatot C.L., KLein N.W., PIatek J., PIerro L.J., (1980) Successful culture of rat embryos in human serum: use in the detection of teratogens, Science, 207, 1471–1473, 1980

    Article  PubMed  CAS  Google Scholar 

  • CIcurel L. and SChmid B.P., Post-implantation embryo culture: validation with selected compounds for teratogenicity testing, Xenobiotica, 18, 617–624,1988a

    Article  PubMed  CAS  Google Scholar 

  • CIcurel L. and SChmid B.P., Postimplantation embryo culture for the assessment of the teratogenic potential and potency of compounds, Experientia, 44, 833–840, 1988b

    Article  PubMed  CAS  Google Scholar 

  • COckroft, Comparison of in vitro and in vivo development of rat foetuses, Developmental Biology, 48, 163–172, 1976

    Article  PubMed  CAS  Google Scholar 

  • DAston G.P. and D’Amato R.A., In vitro techniques in teratology, Toxicology and Industrial Health, 5, 555–585, 1989

    PubMed  CAS  Google Scholar 

  • European Chemical Industry Ecology & Toxicology Centre (ECETOC), Alternative approaches for the assessment of reproductive toxicity (with emphasis on embryotoxicity/teratogenicity), Monograph No 12, 1989

    Google Scholar 

  • FAntel A.G., GReenaway J.C., JUchau M.R., SHepard T.H., Teratogenic biactivation of cyclophosphamide in vitro, Life Sciences, 25, 67–72, 1979

    Article  PubMed  CAS  Google Scholar 

  • FAntel A.G., Culture of whole rodent embryos in teratogen screening, Teratogenesis, Carcinogenesis and Mutagenesis, 2, 231–242, 1982

    Article  CAS  Google Scholar 

  • FAustmann E.M., Short-term tests for teratogens, Mutation Research, 205, 355–384, 1988

    Article  Google Scholar 

  • FLint O.P. and BOyle F.T., An in vitro test for teratogens: its application in the selection of non-teratogenic triazole antifungals, in: HOMBURGER F. (ed.), Concepts in Toxicology, Basel: Karger, 3, 29–35, 1985

    Google Scholar 

  • FLint O.P. and ORton T.C., An in vitro assay for teratogens with cultures of rat embryo midbrain and limb bud cells, Toxicology and Applied Pharmacology, 76, 383–395, 1984

    Article  PubMed  CAS  Google Scholar 

  • FReeman S.J. and BRown N.A., Sub-mammalian and sub-vertebrate models in teratogenicity screening, in: ATterwill C.K. and STeele C.E. (eds.), In vitro methods in toxicology, Cambridge: Cambridge University Press, 391–409, 1987

    Chapter  Google Scholar 

  • FUjinaga M. and BAden J.M., Variation in development of rat embryos at the presomite period, Teratology, 45,661–670, 1992

    Article  PubMed  CAS  Google Scholar 

  • GHaida J. and MErker H.J., Effects of cyclophosphamide and acrolein in organoid cultures of mouse limb bud cells grown in the presence of adult rat hepatocytes, Toxic in Vitro, 6, 27–40, 1992

    Article  CAS  Google Scholar 

  • GOvers M.J.A.P., PEters P.W.J., PIersma A.H., VErhoef A., SChmid B.P., ATtenon J., VAn MAele-FAbry G., PIcard J. J., STadler J., VErseil C., BEchter R., Interlaboratory validation of rodent postimplantation embryo culture as a screening test for teratogenic compounds, Presented at the European Teratology Society Meeting, 1988

    Google Scholar 

  • GRas G. and MOndain J., Pollution des produits de la peche par le mercure et le methylmercure, Toxicol Eur Res, 3, 243–59, 1981

    PubMed  CAS  Google Scholar 

  • GUntakatta M., MAtthews E.J., RUndell J.O., Development of a mouse embryo limb bud cell culture system for the estimation of chemical teratogenic potential, Teratogenesis, Carcinogenesis and Mutagenesis, 4, 349–364, 1984

    Article  CAS  Google Scholar 

  • HAssell J.R. and HOrigan E.A., Chondrogenesis: a model developmental system for measuring teratogenic potential of compounds, Teratogenesis, Carcinogenesis and Mutagenesis, 2, 325–331, 1982

    Article  Google Scholar 

  • HErbst A.L., SCully R.E., RObboy S.J., Prenatal diethylstilbestrol exposure and human genital tract abnormalities, Natl Cancer Inst Monogr, 51, 25–35, 1979

    PubMed  Google Scholar 

  • KImmel G.L., In vitro assays in developmental toxicology: their potential application in risk assessment, in: KImmel G.L. and KOchhar D.M. (eds.), In vitro methods in developmental toxicology: use in defining mechanisms and risk parameters, Boca Raton: CRC Press, 163–173, 1990

    Google Scholar 

  • KIstler A., Inhibition of chondrogenesis by retinoids: limb bud cell cultures as a test system to measure the teratogenic potential of compounds?, in: HOmburger F. (ed), Concepts in Toxicology, Basel: Karger, 3, 86–100, 1985

    Google Scholar 

  • KIstler A., Limb bud cell cultures for estimating the teratogenic potential of compounds–validation of the test system with retinoids, Arch Toxicol, 60, 403–414, 1987

    Article  PubMed  CAS  Google Scholar 

  • KIstler A., TSuchiya T., TSuchiya M., KLaus M., Teratogenicity of arotinoids (retinoids) in vivo and in vitro, Arch Toxicol, 64, 616–622, 1990

    Article  PubMed  CAS  Google Scholar 

  • KItchin K.T., SChmid B.P., SAnyai M.K., Teratogenicity of cyclophosphamide in a coupled microsomal activating/embryo culture system, Biochemical Pharmacology, 30, 59–64, 1981

    Article  PubMed  CAS  Google Scholar 

  • KItchin K.T. and EBron M.T., Further development of rodent whole embryo culture: solvent toxicity and water insoluble compound delivery system, Toxicology, 30, 45–57, 1984

    Article  PubMed  CAS  Google Scholar 

  • KLein N.W., PLenefisch J.D., CArey S.W., FRedrickson W.T., SAckett G.P., BUrbacher T.M., PArker R.M., Serum from monkeys with histories of fetal wastage causes abnormalities in cultures of whole rat embryos, Science, 215, 66–69, 1982

    Article  PubMed  CAS  Google Scholar 

  • KLug S., LEwandowski C., NEubert D., Modification and standardization of the culture of early postimplantation embryos for toxicological studies, Arch Toxicol, 58, 84–88, 1985

    Article  PubMed  CAS  Google Scholar 

  • KOchhar D.M., The use of in vitro procedures in teratology, Teratology, 11,273–288, 1975

    Article  PubMed  CAS  Google Scholar 

  • KOelman H.J.S., JOngeling A.J., Van ERp Y.H.M., WEterings P.J.M., KOopmans M.E., JOosten H.F., V.D. DObbelsteen D.J., V.D. AA E.M., YIh T.D., International ring validation of the in vitro micromass teratogenicity test: preliminary results of 3 dutch laboratories, Teratology, 44, 30A/P39, 1991

    Google Scholar 

  • KOeter H.B.W.M., Test guideline development and animal welfare: regulatoiy acceptance of in vitro studies, Reproductive Toxicology, 7, 117–123, 1993

    Article  PubMed  Google Scholar 

  • KUcera P., CAno E., HOnegger P., SChilter B., ZIjlstra J.A., SChmid B., Validation of whole chick embryo cultures, whole rat embryo cultures and aggregating embryonic brain cell cultures using six pairs of coded compounds, Toxic in Vitro, 7, 785–798, 1993

    Article  CAS  Google Scholar 

  • MAnson J., An overview and comparison of reproductive and developmental toxicity regulations, in: LUmley C.E. and WAlker S.R. (eds.), Current issues in reproductive and developmental toxicology - can an international guideline be achieved?, Lancaster: Quay Publishing, 21–34, 1991

    Google Scholar 

  • NAya M., KIto Y., ETo K., DEguchi T., Development of rabbit whole embryo culture during organogenesis, Congenital Anomalies, 31, 153–156, 1991

    Article  Google Scholar 

  • NEw D.A.T., Whole embiyo culture and the study of mammalian embryos during organogenesis, Biol Rev, 53, 81–122, 1978

    Article  PubMed  CAS  Google Scholar 

  • NInomiya H., KIshida K., OHno Y., TSurumi K., ETo K., Effects of trypan blue on rat and rabbit embryos cultured in vitro, Toxic in Vitro, 7, 707–717, 1993

    Article  CAS  Google Scholar 

  • OGlesby L.A., EBron-MCcoy M.T., LOgsdon T.R., COpeland F., BEyer P.E., KAvlock R.J., In vitro embryotoxicity of a series of para-substituted phenols: structure, activity, and correlation with in vivo data, Teratology, 45, 11–33, 1992

    Article  PubMed  CAS  Google Scholar 

  • PAlmer A.K., Introduction to (pre)screening methods, Reproductive Toxicology, 7, 95–98, 1993

    Article  PubMed  Google Scholar 

  • PAulsen D.F. and SOlursh M., Microtiter micromass cultures of limb-bud mesenchymal cells, In Vitro Cellular & Developmental Biology, 24, 138–147, 1988

    CAS  Google Scholar 

  • PEters P.W.J, and PIersma A.H., In vitro embryotoxicity and teratogenicity studies, Toxic in Vitro, 4, 570–576, 1990

    Article  CAS  Google Scholar 

  • PIersma A.H., ATtenon P., BEchter R., GOvers M.J.A.P., KRafft N., SChmid B.P., STadler J., VErhoef A., VErseil C., Interlaboratoiy evaluation of embiyotoxicity of eight xenobiotic compounds in the postimplantation rat embryo culture, Reproductive Toxicology, 9(3), 275–280, 1995

    Article  PubMed  CAS  Google Scholar 

  • REnault J.Y., MElcion C, COrdier A., Limb bud cell culture for in vitro teratogen screening: validation of an improved assessment method using 51 compounds, Teratogenesis, Carcinogenesis and Mutagenesis, 9, 83–96, 1989

    Article  CAS  Google Scholar 

  • REnault J.Y., GUillet V., GUittin P., Thalidomide activity in a micromass rabbit embryo limb bud cell culture assay, Poster presented at the 21st Annual Conference of the European Teratology Society, Lyon, France, Teratology, 48, 32A, 1993

    Google Scholar 

  • SAdler T.W., Culture of early somite mouse embryos during organogenesis, J Embryol exp Morph, 49, 17–25, 1979

    PubMed  CAS  Google Scholar 

  • SAdler T.W., HOrton W.E., WArner C.W., Whole embryo culture: a screening technique for teratogens?, Teratogenesis, Carcinogenesis and Mutagenesis, 2, 243–253, 1982

    Article  CAS  Google Scholar 

  • SAdler T.W., WArner C.W., TUus S.A., SMith M.K., DOerger J., Factors determining the in vitro response of rodent embryos to teratogens, in: HOmburger F. (ed.), Concepts in Toxicology, Basel: Karger, vol 3, 36–45, 1985

    Google Scholar 

  • SAdler T.W., SHum L., WArner C.W., SMith M.K., The role of pharmacokinetics in determining the response of rodent embryos to teratogens in whole-embryo culture, Toxic in Vitro, 2, 175–180, 1988

    Article  CAS  Google Scholar 

  • SChmid B., BEchter R., KUcera P., The use of whole embryo culture in in vitro tratogenicity testing, in: CAstell J.V. (ed.), In vitro Methods for pharmacotoxicological research, Pergamon Press, 1996, in press

    Google Scholar 

  • SChmid B.P., Teratogenicity testing of new drugs with the postimplantation embryo culture system, in: HOmburger F. (ed.), Concepts in Toxicology, Basel: Karger, vol 3, 46–57, 1985

    Google Scholar 

  • SChmid B.P., TRippmacher A., Bianchi A., Teratogenicity induced in cultured rat embryos by the serum of procarbazine treated rats, Toxicology, 25, 53–60, 1982

    Article  PubMed  CAS  Google Scholar 

  • SChmid B.P., TRippmacher A., BIanchi A., Validation of the whole-embryo culture method for in vitro teratogenicity testing, in: HAyes A.W., SChnell R.C., MIyat.S. (eds.), Developments in the science and practice of toxicology, Amsterdam, New York, Oxford: Elsevier Science Publishers, 563–566, 1983

    Google Scholar 

  • STahlmann R., KLug S., FOerster M., NEubert D., Significance of embryo culture methods for studying the prenatal toxicity of virustatic agents, Reproductive Toxicology, 7, 129–143, 1993

    Article  PubMed  CAS  Google Scholar 

  • TErlouw G.D.C., NAmkung M.J., JUchau M.R., BK R., In vitro embryotoxicity of N-methyl- N-(7-propoxynaphthalene-2-ethyl) hydroxylamine (QAB): evidence for N-dehydroxylated metabolite as a proximate dysmorphogen, Teratology, 48, 431–439, 1993

    Article  PubMed  CAS  Google Scholar 

  • TSuchiya T., BüRgin H., TSuchiya M., WInternitz P., KIstler A., Embryolethality of new herbicides is not detected by the micromass teratogen tests, Arch Toxicol, 65, 145–149, 1991

    Article  PubMed  CAS  Google Scholar 

  • UPhillP.F., WIlkins S.R., ALlen J. A., In vitro micromass teratogen test: results from a blind trial of 25 compounds, Toxic in Vitro, 4, 623–626, 1990

    Article  CAS  Google Scholar 

  • VAn MAele-FAbry G., PIcard J.J., ATtenon P., BErthet P., DElhaise F., GOvers M.J.A.P., PEters P.W.J., PIersma A.H., SChmid B.P., STadler J., VErhoef A., VErseil C., Interlaboratory evaluation of three culture media for postimplantation rodent embryos, Reproductive Toxicology, 5,417–426, 1991

    Article  PubMed  Google Scholar 

  • VAn MAele-FAbry G., THerasse P., LEnoir E., DEsager J.P., DEspontin K., GOfflot G., JAcobs M.C., LEcart C, BErthet P., LAchapelle J.M., PIcard J.J., Embryotoxicity of human sera from patients treated with isotretinoin, Toxic in Vitro, 7, 809–815, 1993a

    Article  Google Scholar 

  • VAn MAele-FAbry G., DElhaise F., GOfflot F., PIcard J.J., Developmental table of the early mouse post-implantation embryo, Toxic in Vitro, 7, 719–725, 1993b

    Article  Google Scholar 

  • WElsch F., Short-term methods of assessing developmental toxicity hazard - status and critical evaluation, in: KAlter H. (ed.), Issues and reviews in teratology, New York: Plenum Press, vol. 5, 115–153, 1990

    Chapter  Google Scholar 

  • WHittaker S.G. and FAustmann E.M., In vitro assays for developmental toxicity, in: GAd S.C. (ed.), In vitro toxicology, New York: Raven Press, 97–122, 1994

    Google Scholar 

  • WIlk A.L., GReenberg J.H., HOrigan E.A., PRatt R.M., MArtin G.R., Detection of teratogenic compounds using differentiating embryonic cells in culture, In Vitro, 16, 269–276, 1980

    Article  PubMed  CAS  Google Scholar 

  • WIlliams P.D., Scientific and regulatory considerations in the development of in vitro techniques in toxicology, in: GAd S.C. (ed.) In vitro toxicology, New York: Raven Press, 225–261, 1994

    Google Scholar 

  • ZHao J., KRafft N., TErlouw G.D.C., BEchter R., A model combining the whole embryo culture with human liver S-9 fraction for human teratogenic prediction, Toxic in Vitro, 7, 827–831, 1993

    Article  CAS  Google Scholar 

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Authors
  1. R. Bechter
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Editors and Affiliations

  1. zet-Zentrum für Ersatz und Ergänzungsmethoden zu Tierversuchen, A-Linz, Austria

    Harald Schöffl  & Helmut A. Tritthart  & 

  2. ZEBET-Zentralstelle zur Erfassung und Bewertung von Ersatz- und Ergänzungsmethoden zum Tierversuch im BgVV - Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, D-Berlin, Germany

    Horst Spielmann

  3. Institut für Medizinische Physik und Biophysik, Karl-Franzens-Universität, A-Graz, Austria

    Helmut A. Tritthart

  4. PEI — Paul-Ehrlich-Institut, D-Langen, Germany

    Klaus Cußler

  5. Stiftung für das Tier im Recht, CH-Zürich, Switzerland

    Antoine F. Goetschel

  6. FFVFF — Fonds für versuchstierfreie Forschung, CH-Zürich, Switzerland

    Franz P. Gruber

  7. SAAT—Swiss Alternatives to Animal Testing, CH-Bertschikon-Zürich, Switzerland

    Christoph A. Reinhardt

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Bechter, R. (1997). Die Anwendung von in vitro-Embryotoxizitätstests in der pharmazeutisch-chemischen Industrie. In: Schöffl, H., et al. Forschung ohne Tierversuche 1996. Ersatz- und Ergänzungsmethoden zu Tierversuchen. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6833-2_11

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  • DOI: https://doi.org/10.1007/978-3-7091-6833-2_11

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