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Western immunoblotting as a method for the detection of African horse sickness virus protein-specific antibodies: Differentiation between infected and vaccinated horses

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African Horse Sickness

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Summary

A Western immunoblotting procedure has been developed for the detection of African horse sickness virus (AHSV) protein-specific antibody responses. This assay readily identifies antibodies specific for at least 4 distinct, AHSV proteins, including VP5, NS1, NS2 and NS3/NS3a. By using the AHSV non-structural proteins as ‘markers’, the Western blotting procedure could be employed to provide a reliable means of discriminating between animals vaccinated with a purified, inactivated AHSV vaccine and those either naturally infected or vaccinated with a live, attenuated AHSV vaccine.

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Authors and Affiliations

  1. Départment de Microbiologie Immunologie Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II, B.P. 6202-Instituts, 10101, Rabat, Morocco

    Dr. S. Idrissi Bougrine

  2. Département de Microbiologie Immunologie Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco

    O. Fassi Fihri & M. M. Fassi Fehri

Authors
  1. Dr. S. Idrissi Bougrine
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  2. O. Fassi Fihri
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  3. M. M. Fassi Fehri
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Editor information

Editors and Affiliations

  1. Pirbright Laboratory, Institute for Animal Health, Pirbright, Woking, Surrey, UK

    Philip S. Mellor , Matthew Baylis , Christopher Hamblin  & Peter P. C. Mertens , ,  & 

  2. Arthropod-borne and Infectious Diseases Laboratory, Colorado State University, Fort Collins, Colorado, USA

    Charles H. Calisher

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© 1998 Springer-Verlag/Wien

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Bougrine, S.I., Fassi Fihri, O., Fassi Fehri, M.M. (1998). Western immunoblotting as a method for the detection of African horse sickness virus protein-specific antibodies: Differentiation between infected and vaccinated horses. In: Mellor, P.S., Baylis, M., Hamblin, C., Mertens, P.P.C., Calisher, C.H. (eds) African Horse Sickness. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6823-3_29

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  • DOI: https://doi.org/10.1007/978-3-7091-6823-3_29

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-83132-8

  • Online ISBN: 978-3-7091-6823-3

  • eBook Packages: Springer Book Archive

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