Extracts of Ginkgo Biloba and Panax Ginseng Protect Brain Proteins from Free Radical Induced Oxidative Damage in Vitro

  • M. S. Siddique
  • F. Eddeb
  • D. Mantle
  • A. D. Mendelow
Conference paper
Part of the Acta Neurochirurgica Supplements book series (NEUROCHIRURGICA, volume 76)


Oxidative damage to normal human brain tissue was induced following exposure to hydroxyl (OH) or superoxide (O2 -•) free radical species generated by CO60 irradiation in vitro. Both enzymic and cytoskeletal proteins showed substantial (dose dependent) oxidative damage following exposure to OH• or O2 -•, as quantified by SDS-polyacrylamide gel electrophoretic analysis. Extracts of Ginkgo biloba or Panax ginseng showed a remarkable capacity to protect brain tissue proteins from oxidative damage in vitro, even at extreme (2000 kRads) dosage levels of OH• or O2 -•. We suggest, therefore, that the beneficial effect of these plant extracts in preventing brain tissue damage in vivo (e.g. following ischemia-reperfusion) may result from their action in protecting brain proteins from oxidative damage, in addition to their previously reported capacity to reduce free radical induced lipid peroxidation.


Free radicals antioxidants protein oxidation brain tissue medicinal plants ginkgo biloba panax ginseng 


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  1. 1.
    Bors W, Heiser W, Michel C, Saran M (1990) Flavonoids as antioxidants: determination of radical scavenging efficiencies. Meth Enzymol 186: 343–355PubMedCrossRefGoogle Scholar
  2. 2.
    Choi SR, Saji H, Iida Y, Magat Y, Yokoyama A (1996) Ginseng pretreatment protects against global cerebral ischemia in the rat: measurement of local cerebral glucose utilization by [14C] deoxyglucose autoradiography. Biol Pharmaceut Bull 19: 644–646CrossRefGoogle Scholar
  3. 3.
    Davies KJA (1987) Protein damage and degradation by oxygen radicals. I. General aspects. J Biol Chem 262: 9895–9906PubMedGoogle Scholar
  4. 4.
    Deby C, Deby-Dupont G, Dister M, Pincemail J (1993) Efficiency of Ginkgo biloba extract (EGB 761) in neutralizing ferryl ion induced peroxidation: therapeutic implications. Adv Ginkgo biloba Ext Res 2: 13–26Google Scholar
  5. 5.
    Dorman DC, Cole LM, Buck WB (1992) Effects of an extract of Ginkgo biloba on bromothalein induced cerebral lipid peroxidation and oedema in rats. Am J Vet Res 53: 138–142PubMedGoogle Scholar
  6. 6.
    Fotun A, Khalil A, Rousselot DB, Albert MG, Lepage MG, Lepage S, Delattre J, Lefaiz MTD. (1994) Effect of EGB 761 on the peroxidation of human low density lipoproteins inhibited by oxyradical generated by water dialysis. J Chim Phys 91: 1078–1084Google Scholar
  7. 7.
    Franzini E, Sellak H, Hakim J, Pasquier C (1993) Oxidative damage to lysosyme by the hydroxyl radical: comparative effects of scavengers. Biochim Biophys Acta 1203: 11–17PubMedCrossRefGoogle Scholar
  8. 8.
    Fricke H, Hart EJ (1966) Radiation dosimetry. In: Roesch FH, Roesch WC (eds) Radiation dosimetry. Academic Press, New York, p 167Google Scholar
  9. 9.
    Garg RK, Nag D, Agrawal A (1995) A double blind placebo controlled trial of Ginkgo biloba extract in acute cerebral ischaemia. J Assoc Physic India 43: 760–763Google Scholar
  10. 10.
    Halliwell B, Gutteridge JMC (1990) Role of free radicals and catalytic metal ions in human disease - an overview. Meth Enzymol 186: 1–85PubMedCrossRefGoogle Scholar
  11. 11.
    Laemli UK (1970) Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227: 680–685CrossRefGoogle Scholar
  12. 12.
    Ni Y, Zhao B, Hou J, Xin W (1996) Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals. Neurosci Lett 214: 115–118PubMedCrossRefGoogle Scholar
  13. 13.
    Oyama Y, Chi Kahisa L, Ueha T, Kanemaru K, Noda K (1996) Ginkgo biloba extract protects brain neurons against oxidative stress induced by hydrogen peroxide. Brain Res 712: 349–352PubMedCrossRefGoogle Scholar
  14. 14.
    Perry EK (1997) Cholinergic phytochemicals: from magic to medicine. Aging Mental Health 1: 23–32CrossRefGoogle Scholar
  15. 15.
    Puchala M, Schuessler H (1993) Oxygen effect in the radiolysis of proteins and haemoglobin. J Radiat Biol 64: 149–156CrossRefGoogle Scholar
  16. 16.
    Seif-El-Nasr M, El-Fattah AA (1995) Lipid peroxide, phospholipids, glutathione levels and superoxide dismutase activity in rat brain after ischaemia: effect of Ginkgo biloba extract. Pharmacol Res 32: 273–278PubMedCrossRefGoogle Scholar
  17. 17.
    Wang FZ, Ding AS, Liu ZW (1995) Effect of ginsenosides against anoxic damage of hippocampal neurons in culture. Chung-Kuo Yao Li Hsueh Pao. Acta Pharmacol Sinica 16:419–422Google Scholar
  18. 18.
    Zhang YG, Liu TP (1996) Influences of ginsenosides Rbl and Rgl on reversible focal brain ischaemia in rats. Chung-Kuo Yao Li Hsueh Pao. Acta Pharmacol Sinica 17: 44–48Google Scholar

Copyright information

© Springer-Verlag Wien 2000

Authors and Affiliations

  • M. S. Siddique
    • 1
  • F. Eddeb
    • 2
  • D. Mantle
    • 2
  • A. D. Mendelow
    • 1
  1. 1.Department of Neurosurgery, Regional Neurosciences CentreNewcastle General HospitalUK
  2. 2.Department of Neurochemistry, Regional Neurosciences CentreNewcastle General HospitalUK

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