Skip to main content

Biological Functions of Extravasated Serum IgG in Rat Brain

  • Conference paper
Brain Edema XI

Part of the book series: Acta Neurochirurgica Supplements ((NEUROCHIRURGICA,volume 76))


During blood-brain barrier opening serum IgG could be extravasated. The function of intraparenchymal IgG, however, is unknown. Its biological effects in the acute phase were currently investigated.

From rat autoserum IgG was purified and injected into the cortex. Similarly, IgG-Fab fragment was prepared and administered like-wise. As for the control group, only vehicle was injected. Animals were sacrificed on days 1,2 and 4 after the infusion and were histologically evaluated.

On days 1 and 2, the infusion of IgG caused significant intraparenchymal infiltration of neutrophils which expressed LFA-1-alpha. It also induced CR3 up-regulation in microglia and endothelial ICAM-1 expression. On day 4, these findings had disappeared. HE stained brain sections and the TUNEL method did not reveal significant nerve cell death in IgG injected animals during the experiment as compared to the controls. IgG-Fab did not cause significant changes either.

Extravasated IgG has been viewed to have biochemical functions. Its Fc fragment seemed to cause microglial and endothelial activation, followed by leukocytic infiltration. This sequence itself was not neurotoxic. Therefore, it is suggested that extravasated IgG is one of the inducers that modulate cellular responses in the acute phase of brain damage.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others


  1. Akopov S, Sercombe R, Seylaz J (1996) Cerebrovascular reactivity: role of endothelium/platelet/leukocyte interactions. Cerebrovasc Brain Metab Rev 8: 11–94

    PubMed  CAS  Google Scholar 

  2. Aspey BS, Jessimer C, Pereira S, Harrison MJG (1989) Do leukocytes have a role in the cerebral no-reflow phenomenon? J Neurol Neurosurg Psychiatry 52: 526–528

    Article  PubMed  CAS  Google Scholar 

  3. Barzo P, Marmarou A, Fatouros P, Corwin F, Dunbar JG (1997) Acute blood-brain barrier changes in experimental closed head injury as measured by MRI and Gd-DTPA. Acta Neurochir [Suppl] (Wien) 70: 243–246

    CAS  Google Scholar 

  4. Beck J, Stummer W, Lehmberg J, Baethmann A, Uhl E (1997) Leukocyteendothelium interactions in global cerebral ischemia. Acta Neurochir [Suppl] (Wien) 70: 53–55

    CAS  Google Scholar 

  5. Del Zoppo GJ, Copeland BR, Harker LA, Waltx TA, Zyroff J, Hanson SR, Battenberg E (1986) Experimental acute thrombotic stroke in baboons. Stroke 17: 1254–1265

    Article  PubMed  Google Scholar 

  6. Hayashi H, Honda M, Mibu Y, Yamamoto S Hirashima M (1988) Natural mediators of leukocyte Chemotaxis. In: Sabato GD (ed) Methods in enzymology. Academic Press, New York, pp 140–170

    Google Scholar 

  7. Koshinaga M, Fukushima M, Oshima H, Suma T, Takabata T, Katayama Y (1997) Rapid microglial activation induced by traumatic brain injury is independent of extravasated blood constituents as revealed by using selective cerebral perfusion model in rat. Adv Neurotrauma Res 9: 44–47

    Google Scholar 

  8. Kushner I (1982) The phenomenon of the acute phase response. Ann NY Acad Sci 389: 39–48

    Article  PubMed  CAS  Google Scholar 

  9. Okada Y, Copeland BR, Mori E, Tung MM, Thomas WS, del Zoppo GJ (1994) P-selectin and intercellular adhesion molecule-1 expression after focal brain ischemia and reperfusion. Stroke 25:202–211

    Article  PubMed  CAS  Google Scholar 

  10. Sedmak DD (1991) Expression of IgG Fc receptor antigens in placenta and on endothelial cell in humans. Am J Pathol 138: 175–81

    PubMed  CAS  Google Scholar 

  11. Sharov LY, Jiang VG, Zaloga C, Sabbah NH, Chopp M (1995) Ultrastructural and light microscopic evidence of apoptosis after middle cerebral artery occlusion in the rat. Am J Pathol 146: 1045–1051

    PubMed  Google Scholar 

  12. Vedeler C, Ulvestad E, Grundt I, Conti G, Nyland H, Matre R, Pleasure D (1994) Fc receptor for IgG(FcR) on rat microglia. J Neuroimmunol 49: 19–23

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer-Verlag Wien

About this paper

Cite this paper

Kadota, E. et al. (2000). Biological Functions of Extravasated Serum IgG in Rat Brain. In: Mendelow, A.D., et al. Brain Edema XI. Acta Neurochirurgica Supplements, vol 76. Springer, Vienna.

Download citation

  • DOI:

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-7257-5

  • Online ISBN: 978-3-7091-6346-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics