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Cis and trans elements regulating expression of the varicella zoster virus gI gene

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Immunity to and Prevention of Herpes Zoster

Summary

We have identified cis-and trans-acting elements involved in the VZV 1E62 protein-activated expression of the varicella zoster virus (VZV) gene which encodes the viral gI glycoprotein. The cis-acting elements include a non-canonical TATA box and a novel 19 base pair sequence located just upstream of the TATA element designated the “activating upstream sequence” or AUS. The AUS is a movable element and its presence results in 1E62 activation of a chimeric promoter consisting of the VZV gC TATA box and the gI AUS. We have also determined that the VZV ORF 29 protein modulates the regulatory activity of the 1E62 protein at the gI promoter. In combination with the 1E62 transactivator, it yields a 10 to 15-fold increase in expression over the levels seen with the 1E62 protein alone in T lymphocytes. The upmodulatory activity requires the presence of a 40 base pair sequence, designated the 29RE, which maps between positions -220 and -180 in the gI promoter. In this paper we review these and earlier findings from our laboratories concerning the regulation of the gI promoter.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Markey Center for Microbial Pathogenesis, State University of New York at Buffalo, Buffalo, New York, USA

    H. He, J. Hay & W. T. Ruyechan

  2. Department of Molecular Genetics, M.D. Anderson Cancer Center, University of Texas, Houston, TX, 77030, USA

    D. Boucaud

  3. Department of Microbiology, State University of New York at Buffalo, Buffalo, New York, 14214, USA

    W. T. Ruyechan

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  1. H. He
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  2. D. Boucaud
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  3. J. Hay
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  4. W. T. Ruyechan
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Editors and Affiliations

  1. Department of Pediatrics, College of Physicians & Surgeons, Columbia University, New York, USA

    Anne A. Gershon

  2. Department of Microbiology and Immunology, Stanford University, Stanford, USA

    Ann M. Arvin

  3. Arthropod-borne and Infectious Diseases Laboratory, Colorado State University, Fort Collins, USA

    Charles H. Calisher

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© 2001 Springer-Verlag Wien

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He, H., Boucaud, D., Hay, J., Ruyechan, W.T. (2001). Cis and trans elements regulating expression of the varicella zoster virus gI gene. In: Gershon, A.A., Arvin, A.M., Calisher, C.H. (eds) Immunity to and Prevention of Herpes Zoster. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6259-0_7

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  • DOI: https://doi.org/10.1007/978-3-7091-6259-0_7

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-83555-5

  • Online ISBN: 978-3-7091-6259-0

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