Experimental Cryoimmunology

  • Shigeo Tanaka
  • Tetsuo Ohkuma
  • Zenichiro Ishii


Cryosurgery per se aims at a local effect, namely, destruction in situ of neoplasms resistant to conventional treatments, but it also elicits an immunologic reaction (cryoimmunologic reaction). In animal experiments, production of antibodies in consequence of freezing treatment was first reported by Yantorno et al. (14) and Shulman et al. (9) in rabbit normal accessory glands, and these antibodies were proved to be tissue-or organ-specific and species-specific. Antibodies in serum in syngeneic mice tumor systems were demonstrated by Blackwood et al. (3). And Ablin et al. (2) elicited antibodies reactive with prostatic tissue in man, as demonstrated by the immunofluorescence technique. Further, Mochizuki (6) reported transfer of tumor-specific immunity by infusion of the cryoimmune lymphocytes to other rats, showing establishment of passive antitumor immunization.


Tumor Infiltrate Lymphocyte Freezing Treatment Delay Hypersensitivity Reaction Flammulina Velutipes Blood Cell Culture 
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© Springer-Verlag Wien 2001

Authors and Affiliations

  • Shigeo Tanaka
  • Tetsuo Ohkuma
  • Zenichiro Ishii

There are no affiliations available

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