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Neural stem/progenitor cells survive and differentiate better in PD rats than in normal rats

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Neurosurgical Re-Engineering of the Damaged Brain and Spinal Cord

Part of the book series: Acta Neurochirurgica Supplements ((NEUROCHIRURGICA,volume 87))

Summary

To investigate the effects of grafted neural stem/mesencephalic progenitor cells (NSCs/MP) on rotational behavior of Parkinson’s disease (PD) rats and the influence of intracerebral environment on NSCs/MP, we observed the survival and differentiation of NSCs/ MP transplanted into 6-hydroxydopamine (6-OHDA)-lesioned and intact striatums. NSCs/MP were prepared from E11–15 rats and proliferated in serum-free medium with bFGF for several weeks. One day after being primed with serum/dbcAMP to differentiate, cell suspensions were grafted into 6-OHDA-lesioned and intact striatums respectively. It had been found that NSCs/MP were able to survive better and differentiate into more tyrosine hydroxylase (TH)-positive neurons in 6-OHDA-lesioned striatums than in intact ones, and apomorphine-induced rotations were obviously attenuated in MP graft models. The data suggested that NSCs/MP tend to survive and differentiate into TH-positive neurons in 6-OHDA-lesioned striatums. The data demonstrated that striatums in which DAergic terminals are destroyed by 6-OHDA undergo some changes and thus provide more appropriate conditions for NSCs/MP to differentiate into mature DAergic neurons. Furthermore, the finding that MP had greater relieving effects on rotational behavior than NSCs suggests that NSCs could not be used in clinical therapy of PD unless being induced into MP in vitro before transplantation.

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Author information

Authors and Affiliations

  1. Institute of Neuroscience of Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China

    Z. H. Sun, Y. L. Lai, W. W. Zeng, D. Zhao & Zuoping Xie

  2. Neurosurgical Center of Medical School, Tsinghua University, Beijing, China

    H. C. Zuo

Authors
  1. Z. H. Sun
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  2. Y. L. Lai
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  3. W. W. Zeng
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  4. D. Zhao
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  5. H. C. Zuo
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  6. Zuoping Xie
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Corresponding author

Correspondence to Zuoping Xie .

Editor information

Editors and Affiliations

  1. Department of Neurological Surgery, Nihon University, School of Medicine, Tokyo, Japan

    Y. Katayama

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© 2003 Springer-Verlag Wien

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Sun, Z.H., Lai, Y.L., Zeng, W.W., Zhao, D., Zuo, H.C., Xie, Z. (2003). Neural stem/progenitor cells survive and differentiate better in PD rats than in normal rats. In: Katayama, Y. (eds) Neurosurgical Re-Engineering of the Damaged Brain and Spinal Cord. Acta Neurochirurgica Supplements, vol 87. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6081-7_35

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  • DOI: https://doi.org/10.1007/978-3-7091-6081-7_35

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-7223-0

  • Online ISBN: 978-3-7091-6081-7

  • eBook Packages: Springer Book Archive

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