Summary
We have found previously that hybrid 22-nm HBsAg particles can be created by insertion of short antigenic sequences into the HBV major envelope protein [1]. We have now performed a detailed deletion mutagenesis of the S gene of HBV encoding HBsAg. Deletion of the 51 C-terminal amino acids including most of the third and all of the fourth hydrophobic domain of the S protein did not affect particle assembly and secretion. However, secretion of 22-nm particles was abolished by minor deletions in the N-terminal region. Insertion and deletion/substitution mutants carrying a poliovirus epitope at the N-terminus and the preSl region at the C-terminus have been characterized.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Delpeyroux F, Chenciner N, Lim A, Malpièce Y, Blondel B, Crainic R, van der Werf S, Streeck RE (1986) A poliovirus neutralization epitope expressed on hybrid hepatitis B surface antigen particles. Science 233: 472–475
Delpeyroux F, Chenciner N, Lambert M, Malpièce Y, Streeck RE (1987) Insertions in the hepatitis B surface antigen: Effect on assembly and secretion of 22-nm particles from mammalian cells. Mol Biol 195: 343–350
Eble BE, MacRae DR, Lingappa VR, Ganem D (1987) Multiple topogenic sequences determine the transmembrane orientation of hepatitis B surface antigen. Mol Cell Biol 7: 3591–3601
Authors’ address: Dr. U. Machein, Institute for Medical Microbiology, University of Mainz, D-W-6500-Mainz, Federal Republic of Germany.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag
About this paper
Cite this paper
Machein, U., Nagel, R., Prange, R., Clemen, A., Streeck, R.E. (1992). Deletion and insertion mutants of HBsAg particles. In: De Bac, C., Taliani, G., Gerlich, W.H. (eds) Chronically Evolving Viral Hepatitis. Archives of Virology, vol 4. Springer, Vienna. https://doi.org/10.1007/978-3-7091-5633-9_29
Download citation
DOI: https://doi.org/10.1007/978-3-7091-5633-9_29
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82350-7
Online ISBN: 978-3-7091-5633-9
eBook Packages: Springer Book Archive