The sequence within the two polyadenylation sites of the A4 amyloid peptide precursor stimulates the translation
cDNA probes specific for the A4 amyloid peptide precursor hybridize with a 3.2–3.4 kb mRNA doublet which can be attributed to the use of two polyadenylation sites. Different chimeric mRNAs were synthesized by in vitro transcription of the coding region of the chicken lysozyme or the chloramphenicol acetyl transferase followed by two 3′ untranslated regions of the A4 amyloid peptide precursor mRNA using the two possible polyadenylation sites. In vivo translation of these mRNA constructs in Xenopus oocytes indicates that the long mRNAs using the second polyadenylation site produce higher amounts of proteins as compared to the short mRNAs. This effect on the translation is not related to a higher stability of the long mRNA in oocytes.
KeywordsDown Syndrome Northern Blot Analysis Polyadenylation Site Chimeric RNAs Chicken Lysozyme
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