Structural Specificity of the Carrier for L-Tryptophan in Rat Cerebral Cortex Slices

  • M. E. Kiely
  • T. L. Sourkes
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 15)


The effect of a number of commonly occurring amino acids as well as metabolites originating in the pyrrolase pathway of tryptophan catabolism and in the serotonin pathway have been studied with regard to their effect on the net flux of tryptophan into rat brain cerebral cortex slices. Phenylalanine, p-chlorophenylalanine, tyrosine, 3, 4-dihydroxyphenylalanine and kynurenine as well as leucine, isoleucine and valine all inhibited the net uptake of tryptophan. It was concluded from these studies that tryptophan is transported into the cell via the large neutral amino acid carrier. Compounds lacking the aliphatic side chain carboxyl or α-amino group have no action on tryptophan transport.


Quinolinic Acid Anthranilic Acid Kynurenic Acid Picolinic Acid Large Neutral Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barbosa, E., Joanny, P., Corriol, J.: Uptake of some amino acids by rat brain slices: effect of various substrates. Experientia 24, 1196–1197 (1968).PubMedCrossRefGoogle Scholar
  2. Barbosa, E., Joanny, P., Corriol, J.: Accumulation active du tryptophane dans le cortex cerebral isolé du rat. Comptes rendus des séances de la Société de Biologie 164, 345–350 (1970).Google Scholar
  3. Barbosa, E., Herreros, B., Ojeda, J. L.: Amino acid accumulation by brain slices: interactions among tryptophan, phenylalanine and histidine. Experientia 27, 1281–1282 (1971).PubMedCrossRefGoogle Scholar
  4. Belin, M.-F., Pujol, J.-F.: Transport synaptosomal du tryptophane et de la tyrosine cérébrale. Existence de systèmes de capture d’affinité différente. Experientia 29, 411–413 (1973).Google Scholar
  5. Blasberg, R., Lajtha, A.: Substrate specificity of steady-state amino acid transport in mouse brain slices. Archiv. Biochem. Biophys. 112, 361–377 (1965).CrossRefGoogle Scholar
  6. Blasberg, R., Lajtha, A.: Heterogeneity of the mediated transport systems of amino acid uptake in brain. Brain Res. 1, 86–104 (1966).PubMedGoogle Scholar
  7. Bruinvels, J.: Role of sodium in neuronal uptake of monoamines and amino acid precursors. Nature 257, 606–607 (1975).PubMedCrossRefGoogle Scholar
  8. Denizeau, F., Sourkes, T. L.: Regional transport of tryptophan in rat brain. J. Neurochem. 28, 951–959 (1977).PubMedCrossRefGoogle Scholar
  9. Grabame-Smitb, D. G., Parfitt, A. G.: Tryptophan transport across the synaptosomal membrane. J. Neurochem. 17, 1339–1353 (1970).CrossRefGoogle Scholar
  10. Green, A. R., Curzon, G.: The effect of tryptophan metabolites on brain 5-hydroxytryptamine metabolism. Biochem. Pharmacol. 19, 2061–2068 (1970).PubMedCrossRefGoogle Scholar
  11. Joanny, P., Barbosa, E., Corriol, J.: Accumulation active de quelques acides aminés dans les coupes de cerveau de rat. Journal de Physiologie (Paris) Suppl. 60, 265 (1968).Google Scholar
  12. Joanny, P., Natali, J.-P., Hillman, H., Corriol, J.: The uptake and efflux of L-phenylalanine and L-tyrosine from rat brain cerebral cortex slices. Biochem. J. 136, 77–82 (1973).PubMedGoogle Scholar
  13. Kiely, M., Sourkes, T. L.: Transport of L-tryptophan into slices of rat cerebral cortex. J. Neurochem. 19, 2863–2872 (1972).PubMedCrossRefGoogle Scholar
  14. Lajtha, A., Sershen, H.: Inhibition of amino acid uptake by the absence of Na+ in slices of brain. J. Neurochem. 24, 667–672 (1975).PubMedGoogle Scholar
  15. Neame, K. D.: Progress in Brain Research. In: Brain Barrier Systems. A comparison of the transport systems for amino acids in brain, intestine, kidney and tumor, Vol. 29, p. 185. Amsterdam: Elsevier. 1968.Google Scholar
  16. Pardridge, W. M.: Kinetics of competitive inhibition of neutral amino acid transport across the blood-brain barrier. J. Neurochem. 28, 103–108 (1977).PubMedCrossRefGoogle Scholar
  17. Young, S. N., Sourkes, T. L.: Tryptophan in the central nervous system: regulation and significance. Adv. Neurochem. 2, 133–191 (1977).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 1979

Authors and Affiliations

  • M. E. Kiely
    • 1
  • T. L. Sourkes
    • 1
  1. 1.Department of Psychiatry, The Montreal General HospitalMcGill UniversityMontrealCanada

Personalised recommendations