Sarcocystis spp. and Pentastomes in Southeast Asia

  • John Jeffery
  • Arine F. AhmadEmail author
  • Noraishah M. Abdul-Aziz


Sarcocystis and pentastomes infections are uncommon zoonoses of worldwide distribution. In most of the cases, they were detected as an incidental finding at autopsy or necropsy. Recently, serious attention has been given to Sarcocystis when large human outbreaks have been reported in Malaysia. Similarly, the detection of a recent human pentastomiasis case in East Malaysia since nearly four decades ago has alerted that pentastomes should not be ignored by the medical and laboratory personnel. In addition, understanding the routes of infection is crucial in preventing further cases. This chapter will focus on sarcocystosis and pentastomiasis cases among humans and animals.


Intermediate Host Definitive Host Water Buffalo Southeast Asian Country Indirect Fluorescent Antibody Test 
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  1. 1.
    R. Fayer, Sarcocystis spp. in human infections. Clin. Microbiol. Rev. 17(4), 894–902 (2004), table of contentsGoogle Scholar
  2. 2.
    J.P. Dubey et al., Sarcocystis of Animals and Man (CRC, Boca Raton, FL, 1989)Google Scholar
  3. 3.
    S.P. Kan, R. Pathmanathan, Review of sarcocystosis in Malaysia. Southeast Asian J. Trop. Med. Public Health 22(Suppl), 129–134 (1991)PubMedGoogle Scholar
  4. 4.
    S. Nichpanit et al., First large scale of human Sarcocystis hominis in Thailand. Trends Res. Sci. Technol. 2(1), 1–5 (2010)Google Scholar
  5. 5.
    M.K. Arness et al., An outbreak of acute eosinophilic myositis attributed to human Sarcocystis parasitism. Am. J. Trop. Med. Hyg. 61(4), 548–553 (1999)PubMedGoogle Scholar
  6. 6.
    M.K. Kutty et al., Sarcocystis infection in an Orang Asli: the second human case from Malaysia. Southeast Asian J. Trop. Med. Public Health 6(3), 400–401 (1975)PubMedGoogle Scholar
  7. 7.
    B. Latif et al., Sarcocystosis among wild captive and zoo animals in Malaysia. Korean J. Parasitol. 48(3), 213–217 (2010)PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    S. Ambu et al., Prevalence of Sarcocystis spp. in rodents in Peninsular Malaysia. IeJSME 5(2), 29–38 (2011)Google Scholar
  9. 9.
    K.T. Wong, R. Pathmanathan, High prevalence of human skeletal muscle sarcocystosis in south-east Asia. Trans. R. Soc. Trop. Med. Hyg. 86(6), 631–632 (1992)PubMedCrossRefGoogle Scholar
  10. 10.
    D.S. Lindsay et al., Sarcocystis spp. and Sarcocystosis. BAM 5(3), 249–254 (1995)Google Scholar
  11. 11.
    P.C. Beaver et al., Sarcocystis in man: a review and report of five cases. Am. J. Trop. Med. Hyg. 28(5), 819–844 (1979)PubMedGoogle Scholar
  12. 12.
    M.K. Kutty, A.S. Dissanaike, A case of human Sarcocystis infection in west Malaysia. Trans. R. Soc. Trop. Med. Hyg. 69(5–6), 503–504 (1975)PubMedCrossRefGoogle Scholar
  13. 13.
    R. Pathmanathan, S.P. Kan, Three cases of human Sarcocystis infection with a review of human muscular sarcocystosis in Malaysia. Trop. Geogr. Med. 44(1–2), 102–108 (1992)PubMedGoogle Scholar
  14. 14.
    V. Thomas, A.S. Dissanaike, Antibodies to Sarcocystis in Malaysians. Trans. R. Soc. Trop. Med. Hyg. 72(3), 303–306 (1978)PubMedCrossRefGoogle Scholar
  15. 15.
    CDC, Notes from the field: acute muscular Sarcocystosis among returning travelers Tioman Island, Malaysia, 2011. MMWR Morb. Mortal. Wkly Rep. 61(02), 37–38 (2012)Google Scholar
  16. 16.
    D.H. Esposito et al., Ongoing outbreak of an acute muscular Sarcocystis-like illness among travellers returning from Tioman Island, Malaysia, 2011-2012. Euro Surveill. 17(45), pii: 20310 (2012)Google Scholar
  17. 17.
    S.P. Kan, Ultrastructure of the cyst wall of Sarcocystis spp. from some rodents in Malaysia. Int. J. Parasitol. 9(5), 475–480 (1979)PubMedCrossRefGoogle Scholar
  18. 18.
    S.P. Kan, A.S. Dissanaike, Ultrastructure of Sarcocystis sp. from the Malaysian house rat, Rattus rattus diardii. Z. Parasitenkd. 52(3), 219–227 (1977)PubMedCrossRefGoogle Scholar
  19. 19.
    V. Zaman, F.C. Colley, Light and electron microscopic observations of the life cycle of Sarcocystis orientalis sp. n. in the rat (Rattus norvegicus) and the Malaysian reticulated python (Python reticulatus). Z. Parasitenkd. 47(3), 169–185 (1975)PubMedCrossRefGoogle Scholar
  20. 20.
    A.S. Dissanaike et al., Demonstration of the sexual phases of Sarcocystis fusiformis (Railliet, 1897) and Sarcocystis sp. of the water buffalo (Bubalus bubalis) in the small intestines of cats and dogs. Trans. R. Soc. Trop. Med. Hyg. 71(3), 271 (1977)PubMedCrossRefGoogle Scholar
  21. 21.
    B. Latif et al., Sarcocystosis among cattle and buffaloes in Selangor-Malaysia, in 49th Annual Conference of the Malaysian Society of Parasitology and Tropical Medicine (MSPTM), Kuala Lumpur, 19–20 Mar 2013, p. 23Google Scholar
  22. 22.
    O. Norlida et al., A histopathological appraisal of ovine Sarcocystosis in Kelantan. J. Adv. Biomed. Pathobiol. Res. 2(4), 137–142 (2012)Google Scholar
  23. 23.
    V. Zaman, Sarcocystis sp. in the slow loris, Nycticebus coucang. Trans. R. Soc. Trop. Med. Hyg. 64(1), 195–196 (1970)PubMedCrossRefGoogle Scholar
  24. 24.
    S.P. Kan et al., Light and electron microstructure of a Sarcocystis sp. from the Malaysian long-tailed monkey, Macaca fascicularis. Am. J. Trop. Med. Hyg. 28(4), 634–642 (1979)PubMedGoogle Scholar
  25. 25.
    K. Prathap, Letter: Sarcocystis in the Malaysian long-tailed monkey, Macaca irus. Trans. R. Soc. Trop. Med. Hyg. 67(4), 615 (1973)PubMedCrossRefGoogle Scholar
  26. 26.
    G. Juckett, R. Trivedi, Evaluation of chronic diarrhea. Am. Fam. Physician 84(10), 1119–1126 (2011)PubMedGoogle Scholar
  27. 27.
    S. Bunyaratvej et al., The Sarcocystis-cyst containing beef and pork as the sources of natural intestinal sarcocystosis in Thai people. J. Med. Assoc. Thai. 90(10), 2128–2135 (2007)PubMedGoogle Scholar
  28. 28.
    S. Nateeworanart et al., Prevalence of Sarcocystis spp in cardiac muscle of swine in Samut Prakan Province, Thailand. J. Southeast Asian Educ. 35(1), 82–83 (2004)Google Scholar
  29. 29.
    P. Wilairatana et al., Intestinal sarcocystosis in Thai laborers. Southeast Asian J. Trop. Med. Public Health 27(1), 43–46 (1996)PubMedGoogle Scholar
  30. 30.
    S. Bunyaratvej, P. Unpunyo, Combined Sarcocystis and gram-positive bacterial infections. A possible cause of segmental enterocolitis in Thailand. J. Med. Assoc. Thai. 75(Suppl 1), 38–44 (1992)PubMedGoogle Scholar
  31. 31.
    Y. Muangyimpong et al., Prevalence of Sarcocystis infection in primary school children in Changwat Chiang Mai, Northern Thailand. J. Trop. Med. Parasitol. 16(1), 22–24 (1993)Google Scholar
  32. 32.
    N. Larbcharoensub et al., Laryngeal sarcocystosis accompanying laryngeal squamous cell carcinoma: case report and literature review. Southeast Asian J. Trop. Med. Public Health 42(5), 1072–1076 (2011)PubMedGoogle Scholar
  33. 33.
    T. Jakel, Biological rodent control – TJ Homepage (2006), Accessed 2/8/2013
  34. 34.
    T. Jakel et al., Biological control of rodents using Sarcocystis singaporensis. Int. J. Parasitol. 29(8), 1321–1330 (1999)PubMedCrossRefGoogle Scholar
  35. 35.
    P.J. O’Donoghue et al., Ultrastructure of Sarcocystis spp. (Protozoa: Apicomplexa) in rodents from North Sulawesi and West Java, Indonesia. J. Wildl. Dis. 23(2), 225–232 (1987)PubMedCrossRefGoogle Scholar
  36. 36.
    P.V. Arambulo et al., Sarcosporidiosis in Philippine buffaloes. Philipp. J. Vet. Med. 11, 53–59 (1972)Google Scholar
  37. 37.
    F.G. Claveria, M.J. Cruz, Sarcocystis levinei infection in Philippine water buffaloes (Bubalus bubalis). Parasitol. Int. 48(3), 243–247 (2000)PubMedCrossRefGoogle Scholar
  38. 38.
    M. Tongson, M. Pelagio, A study on the incidence of Sarcocystis (SIC) trophozoites in ground meat sold as beef in Metro Manila. Philipp. J. Vet. Anim. Sci. 4, 253–269 (1978)Google Scholar
  39. 39.
    F.G. Claveria et al., Parasite biodiversity in Rattus spp caught in wet markets. Southeast Asian J. Trop. Med. Public Health 36(Suppl 4), 146–148 (2005)PubMedGoogle Scholar
  40. 40.
    F.G. Claveria, Survey of Sarcocystis spp. infection in Philippine livestock animals: light microscopic and ultrastructural studies. Research@DLSU-Manila: Continuing the Cycle, p. 100 (2007)Google Scholar
  41. 41.
    F.G. Claveria et al., Sarcocystis miescheriana infection in domestic pigs (Sus scrofa) in the Philippines. J. Parasitol. 87(4), 938–939 (2001)PubMedGoogle Scholar
  42. 42.
    F.G. Claveria et al., Sarcocystis capracanis infection in Philippine domestic goats (Capra hircus): ultrastructural studies. Philipp. J. Sci. 133(1), 33–37 (2004)Google Scholar
  43. 43.
    C. Jehle et al., Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam. Vet. Parasitol. 166(3–4), 314–320 (2009)PubMedCrossRefGoogle Scholar
  44. 44.
    L.T. Huong, Prevalence of Sarcocystis spp. in water buffaloes in Vietnam. Vet. Parasitol. 86(1), 33–39 (1999)PubMedCrossRefGoogle Scholar
  45. 45.
    D.D. Bowman et al., Feline Clinical Parasitology (Iowa State University Press, Ames, IA, 2002)CrossRefGoogle Scholar
  46. 46.
    A.M. Baticados, W.N. Baticados, Histopathology of protozoal infection in animals: a retrospective study at the University of Philippines College of Veterinary Medicine (1972-2010). Vet. Ital. 48(1), 99–107 (2012)PubMedGoogle Scholar
  47. 47.
    Y.S. Habeeb et al., Serological diagnosis of extraintestinal Sarcocystosis. J. Egypt. Soc. Parasitol. 26(2), 393–400 (1996)PubMedGoogle Scholar
  48. 48.
    G. Savini et al., Sensitivities and specificities of two ELISA tests for detecting infection with Sarcocystis in cattle of Western Australia. Prev. Vet. Med. 32(1–2), 35–40 (1997)PubMedCrossRefGoogle Scholar
  49. 49.
    Z.Q. Yang et al., Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences. J. Parasitol. 87(4), 934–937 (2001)PubMedGoogle Scholar
  50. 50.
    Z.Q. Yang et al., Analysis of the 18S rRNA genes of Sarcocystis species suggests that the morphologically similar organisms from cattle and water buffalo should be considered the same species. Mol. Biochem. Parasitol. 115(2), 283–288 (2001)PubMedCrossRefGoogle Scholar
  51. 51.
    M. Tian et al., Phylogenetic analysis of Sarcocystis nesbitti (Coccidia: Sarcocystidae) suggests a snake as its probable definitive host. Vet. Parasitol. 183(3–4), 373–376 (2012)PubMedCrossRefGoogle Scholar
  52. 52.
    J. Riley et al., The phylogenetic relationships of the Pentastomida: the case for their inclusion within the crustacean. Int. J. Parasitol. 8, 245–254 (1978)CrossRefGoogle Scholar
  53. 53.
    M. Krishnasamy et al., Some pentastomes from Malaysia, with special emphasis on those from Peninsular Malaysia. J. Malays. Soc. Health 5, 49–56 (1985)Google Scholar
  54. 54.
    J.T. Self et al., Pentastomes in Africa. Trop. Geogr. Med. 27, 1–13 (1975)PubMedGoogle Scholar
  55. 55.
    M.H. Yao et al., Human pentastomiasis in China: case report and literature review. J. Parasitol. 94, 1295–1298 (2008)PubMedCrossRefGoogle Scholar
  56. 56.
    R.P. Dollfus, J. Canet, Sur en Pentastomide Raillietiella hemidactyli suppose susceptible de parasiter l’homme consecutivement a l’ingestion de lezards vivants. Bull. Soc. Pathol. Exot. 47, 401–407 (1954)Google Scholar
  57. 57.
    J.H. Ali, J. Riley, Experimental life-cycle studies of Raillietiella gehyrae Bovien, 1927 and Raillietiella frenatus Ali, Riley and Self. 1981: pentastomid parasites of geckos utilizing insects as intermediate hosts. Parasitology 86, 147–160 (1983)CrossRefGoogle Scholar
  58. 58.
    J. Jeffery et al., Observation on the cockroach intermediate hosts and species and species of house gecko raillietiellid in peninsular Malaysia. J. Malays. Soc. Health 12, 57–65 (1994)Google Scholar
  59. 59.
    C. Rajamanickam, M.M.J. Lavoipierre, Periplaneta australasiae in an intermediate host of the pentastomid Raillietiella hemidactyli. Med. J. Malaya 20, 171 (1965)Google Scholar
  60. 60.
    K. Prathap et al., Pentastomiasis: a common finding at autopsy among Malaysian aborigines. Am. J. Trop. Med. Hyg. 18, 20–27 (1969)PubMedGoogle Scholar
  61. 61.
    J. Prathap et al., Hepatic and pulmonary porocephaliasis in Malaysian Orang Asli (Aborigine). Med. J. Malaya 23, 92–95 (1968)PubMedGoogle Scholar
  62. 62.
    G. Rail, Porocephaliasis: a description of two cases in Sabah. Trans. R. Soc. Trop. Med. Hyg. 61, 715–717 (1967)PubMedCrossRefGoogle Scholar
  63. 63.
    H.C. Ong, An unusual case of pentastomiasis of the fallopian tube in an Aborigine woman. J. Trop. Med. Hyg. 77(8), 187–189 (1974)PubMedGoogle Scholar
  64. 64.
    B. Latif et al., Case report: human pentastomiasis caused by Armillifer moniliformis in Malaysian Borneo. Am. J. Trop. Med. Hyg. 85(5), 878 (2011)PubMedCrossRefGoogle Scholar
  65. 65.
    J. Riley, J.T. Self, Some observation on the taxonomy and systematics of the pentastomid genus Armillifer (Sambos, 1922) in South East Asian and Australian snakes. Syst. Parasitol. 2, 171–179 (1981)CrossRefGoogle Scholar
  66. 66.
    K. Wessendorf, The Indigenous World 2009 (IWGIA, Copenhagen, 2009)Google Scholar
  67. 67.
    K. Haemamalar Jr. et al., Nutritional status of orang asli (che wong tribe) adults in krau wildlife reserve, pahang. Malays. J. Nutr. 16(1), 55–68 (2010). Epub 2010/04/01PubMedGoogle Scholar
  68. 68.
    S. Straka et al., Sarcocystis species in Vietnamese workers. Ceskoslovenska epidemiologie, mikrobiologie, imunologie 40(4–5), 204–8 (1991). Epub 1991/11/01. Sarcocystis species u vietnamskych praktikantovPubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • John Jeffery
    • 1
  • Arine F. Ahmad
    • 1
    Email author
  • Noraishah M. Abdul-Aziz
    • 1
  1. 1.Department of Parasitology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia

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