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Early Changes in GMRP1 After Intracerebral Hemorrhage: Involvement in Brain Damage and Cell Apoptosis

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Brain Edema XV

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 118))

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Abstract

Glucose metabolism-related protein 1 (GMRP1), also known as BTBD10, has been reported to inhibit apoptosis of neuronal and islet beta cells via the Akt pathway. The present study attempted to investigate whether GMRP1 and its mediated Akt pathway were involved in brain injury of rats after intracerebral hemorrhage (ICH). Rat models of ICH had been established successfully. Western blotting was used to investigate the levels of GMRP1 protein in the caudate nuclei tissues of the hemorrhagic and contralateral sides at 6 h, day 1, day 3, day 5, and day 7 after ICH. Phosphorylations of Akt was determined in caudate nuclei mentioned above. TUNEL assay was used to measure the cell apoptosis. GMRP1 protein levels, as well as phosphorylations of Akt, significantly decreased in caudate nuclei of the hemorrhagic side, compared with those of the contralateral side on day 1 and day 3 after ICH. Enhanced cell apoptosis was observed on the hemorrhagic side using TUNEL assay. We presented here evidence that a decreased GMRP1-mediated Akt pathway contributed to cell apoptosis on the hemorrhagic side, suggesting that GMRP1 plays an important role in brain damage after ICH.

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Acknowledgments

This study was supported by Grant No.08411965100 from the Science and Technology Commission of the Shanghai Municipality, and Grant Nos. 30872675 and 30901549 from the National Natural Science Foundation of China.

Conflict of InterestWe declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai, China

    Xuanchun Wang

  2. Department of Neurosurgery, Huashan Hospital, Fudan University, 12 Middle Wurumuqi Road, Shanghai, China

    Ye Gong, Mingzhe Zheng, Qing Xie, Hailiang Tang, Daijun Wang & Xiancheng Chen

Authors
  1. Xuanchun Wang
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  2. Ye Gong
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  3. Mingzhe Zheng
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  4. Qing Xie
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  5. Hailiang Tang
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  6. Daijun Wang
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  7. Xiancheng Chen
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Corresponding author

Correspondence to Ye Gong .

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Editors and Affiliations

  1. School of Medicine, Dept. Neurological Surgery, Nihon University, Oyaguchikami-machi 30-1, Tokyo, 173-8610, Japan

    Yoichi Katayama

  2. School of Medicine, Departments of Neurological Surgery and, Nihon University, 30-1 Oyaguchi Kamimachi, Itabashi-ku, Tokyo, 173-8610, Japan

    Takeshi Maeda

  3. , Department of Clinical Laboratory, Namegata District General Hospital, 98-8 Inouefujii, Namegata 98-8, Ibaraki, 311-3516, Japan

    Toshihiko Kuroiwa

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Wang, X. et al. (2013). Early Changes in GMRP1 After Intracerebral Hemorrhage: Involvement in Brain Damage and Cell Apoptosis. In: Katayama, Y., Maeda, T., Kuroiwa, T. (eds) Brain Edema XV. Acta Neurochirurgica Supplement, vol 118. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1434-6_30

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  • DOI: https://doi.org/10.1007/978-3-7091-1434-6_30

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-1433-9

  • Online ISBN: 978-3-7091-1434-6

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