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Subunit Vaccine Candidates Engineered from the Central Conserved Region of the RSV G Protein Aimed for Parenteral or Mucosal Delivery

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Molecular Vaccines

Abstract

Respiratory syncytial virus (RSV) is a major pathogen causing severe upper and lower respiratory disease in infants and in elderly worldwide. According to WHO, an RSV vaccine is urgently needed. Here, we describe the design of various types of subunit vaccine concepts based on molecular engineering aimed to deliver RSV antigens. Gene segment encoding parts of the conserved central region of the RSV G protein (G2Na) were prepared for various expression and delivery formats: (1) prokaryotically expressed and purified G2Na alone or fused to different carrier proteins, one of them, namely, BBG2Na (Alum), has reached clinical trials in the elderly; (2) G protein-derived antigens surface displayed on lived vectors (non pathogenic bacteria) and (3) nucleic acid vectors.

These subunit vaccines were administered with or without adjuvant in rodents and in non-human primates by different routes (parenteral or mucosal). We summarise and compare immunogenicity, protective efficacy and safety conferred by each immunisation format in RSV experimental animal models. Among these, G2Na proved to be the most promising component for an RSV subunit vaccine.

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Acknowledgments

C Andersson, M Azin, T Baussant, A Beck, H Binz, JF Boe, JY Bonnefoy, N Bouveret-LeCam, JF Cano, B Chol, JC Corbiere, N Corvaia, N Dagouassat, F Farinole, L Goetsch, M Hansson, JF Haeuw, K Helffer, T Huss, C Issac, C Klinguer, PH Lambert, M Lokteff, S Liljeqvist, M Maitre, C Nadon, Q Nguyen, PA Nygren, H Plotnicky-Gilquin, A Perez, U Power, A Robert, P Samuelsson, CA Siegrist, C Simard, M Trudel, M Uhlén, D Velin and L Zanna.

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Authors and Affiliations

  1. Department of Microbiotechnology, Institut de Recherche Pierre Fabre, Toulouse, France

    Thien N. Nguyen PhD & Christine Libon PhD

  2. Division of Molecular Biotechnology, School of Biotechnology, Alba Nova University Center, KTH Royal Institute of Technology, Stockholm, Sweden

    Stefan Ståhl PhD (Dean)

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  1. Thien N. Nguyen PhD
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  2. Christine Libon PhD
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Correspondence to Thien N. Nguyen PhD .

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  1. Institute for Molecular Vaccines, Heidelberg, Germany

    Matthias Giese

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Nguyen, T.N., Libon, C., Ståhl, S. (2013). Subunit Vaccine Candidates Engineered from the Central Conserved Region of the RSV G Protein Aimed for Parenteral or Mucosal Delivery. In: Giese, M. (eds) Molecular Vaccines. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1419-3_5

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