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Combined Systemic Thrombolysis with Alteplase and Early Hyperbaric Oxygen Therapy in Experimental Embolic Stroke in Rats: Relationship to Functional Outcome and Reduction of Structural Damage

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Book cover Intracerebral Hemorrhage Research

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 111))

Abstract

Introduction: The only causal therapy in ischemic stroke is thrombolysis with recombinant tissue plasminogen activator (rtPA), but it is feasible only for few patients, and new therapies are needed. This study investigates the effects of systemic thrombolysis with rtPA combined with hyperbaric oxygen therapy (HBOT) in embolic stroke in rats.

Methods: In 22 male Wistar rats, an embolic ischemic stroke was induced. The animals were randomized to one of four groups: control, thrombolysis alone, HBOT sequential or HBOT parallel with thrombolysis. HBOT (2.4 ATA, 1 h) started 45 min (sequential) or 120 min (parallel) after stroke. rtPA was given intravenously 120 min after stroke onset. Functional tests were performed after stroke induction and after treatment. After 6 h infarct volume and intracerebral hemorrhagic complications were assessed.

Results: Compared to the control group only the combination of HBOT and thrombolysis significantly improved the functional outcome (p = 0.03) and reduced the infarct volume (p = 0.01), whereas thrombolysis alone did not show a significant benefit. In all treatment groups there was a trend towards fewer hemorrhagic transformations.

Conclusion: Hyperbaric oxygen in combination with thrombolysis shows neuroprotection in acute ischemic stroke in rats by reducing infarct volume and improving functional outcome in the early poststroke period.

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Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurology, University of Leipzig, Liebigstraße 20, 04103, Leipzig, Germany

    Lea Küppers-Tiedt, Anatol Manaenko, Dominik Michalski, Albrecht Guenther, Carsten Hobohm, Armin Wagner & Dietmar Schneider

  2. Department of Physiology and Pharmacology, Loma Linda University, School of Medicine, Loma Linda, CA, USA

    Anatol Manaenko & John H. Zhang

Authors
  1. Lea Küppers-Tiedt
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  2. Anatol Manaenko
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  3. Dominik Michalski
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  4. Albrecht Guenther
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  5. Carsten Hobohm
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  6. Armin Wagner
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  7. John H. Zhang
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  8. Dietmar Schneider
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Correspondence to Lea Küppers-Tiedt or Anatol Manaenko .

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Editors and Affiliations

  1. , Neurosurgery, Physiology, Loma Linda University, Campus Street 11041, Loma Linda, 92530, USA

    John Zhang

  2. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    Austin Colohan

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Küppers-Tiedt, L. et al. (2011). Combined Systemic Thrombolysis with Alteplase and Early Hyperbaric Oxygen Therapy in Experimental Embolic Stroke in Rats: Relationship to Functional Outcome and Reduction of Structural Damage. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_28

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  • DOI: https://doi.org/10.1007/978-3-7091-0693-8_28

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0692-1

  • Online ISBN: 978-3-7091-0693-8

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