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Hemoglobin Expression in Neurons and Glia After Intracerebral Hemorrhage

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Book cover Intracerebral Hemorrhage Research

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 111))

Abstract

The purpose of this study was to examine the expression of hemoglobin (Hb) in the brain after intracerebral hemorrhage (ICH) and the effects of hemin and iron on neuronal Hb.

For the in vivo studies, male Sprague-Dawley rats received either a sham operation or an ICH. The rats were killed 1, 4, 24 or 72 h later, and brains were used for real-time polymerase chain reaction (PCR) and immunohistochemistry. For the in vitro study, primary cultured neurons were exposed to either hemin or vehicle. Some neurons also received treatment with deferoxamine, an iron chelator. Neurons were collected 24 h later for real-time PCR.

We found that α-globin (HbA) and β-globin (HbB) mRNA levels in the ipsilateral basal ganglia are significantly increased after ICH, and Hb is localized in neurons and glia cells. Exposure of neurons to hemin also upregulated HbA and HbB mRNA levels. Hemin-induced HbA and HbB expression in cultured neurons was reduced by deferoxamine treatment.

These results indicate that ICH increases HbA and HbB expression in neurons and glia cells, and that heme and iron may be important factors in inducing endogenous Hb expression after ICH.

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Acknowledgements

This study was supported by grants NS-017760, NS-039866 and NS-057539 from the National Institutes of Health (NIH) and 0755717Z and 0840016N from the American Heart Association (AHA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and AHA. MMW is supported by NS-062816 and NS-054724.

Conflict of interest statement We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, University of Michigan, Ann Arbor, MI, USA

    Yangdong He, Ya Hua, Richard F. Keep, Wenquan Liu & Guohua Xi

  2. Department of Neurology, University of Michigan, Ann Arbor, MI, USA

    Michael M. Wang

  3. Department of Neurosurgery, University of Michigan, R5018 Biomedical Science Research Building, Ann Arbor, 48109-2200, MI, USA

    Guohua Xi

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  1. Yangdong He
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  2. Ya Hua
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  3. Richard F. Keep
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  4. Wenquan Liu
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  5. Michael M. Wang
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  6. Guohua Xi
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Corresponding author

Correspondence to Guohua Xi .

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Editors and Affiliations

  1. , Neurosurgery, Physiology, Loma Linda University, Campus Street 11041, Loma Linda, 92530, USA

    John Zhang

  2. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    Austin Colohan

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© 2011 Springer-Verlag/Wien

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He, Y., Hua, Y., Keep, R.F., Liu, W., Wang, M.M., Xi, G. (2011). Hemoglobin Expression in Neurons and Glia After Intracerebral Hemorrhage. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_22

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  • DOI: https://doi.org/10.1007/978-3-7091-0693-8_22

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0692-1

  • Online ISBN: 978-3-7091-0693-8

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