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The Glycoproteins of Giardia

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Giardia

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Abstract

Glycoprotein structures are remarkably simple in Giardia. This protist produces the shortest Asn-linked glycan (N-glycan) yet described: two N-acetyl-glucosamines (GlcNAc2). The oligosaccharyltransferase (OST) that transfers N-glycans to the peptide has a single catalytic subunit in Giardia but contains four to eight subunits in most eukaryotes. Giardia is missing the ER proteins involved in N-glycan-dependent quality conrol (QC) of protein folding and degradation. There is Darwinian selection for the sites of N-glycan in secreted proteins of eukaryotes with N-glycan-dependent QC, but there is no such selection in Giardia and other protists lacking N-glycan-dependent QC. The glycosylphosphatidylinositol (GPI) anchor of Giardia is predicted to be the simplest of any eukaryote. UDP-GlcNAc is the only nucleotide sugar transported from the cytosol to the lumen of the ER. By contrast, Giardia is one of the rare protists that use GlcNAc to modify Ser and Thr residues on nucleocytosolic proteins. WGA affinity dramatically enriches glycoproteins of Giardia, many of which are unique or are encystation specific. In summary, GlcNAc is the major sugar added to Giardia glycoproteins, which are much less complex than those of the host.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, Boston University, Boston, MA, 02118-2394, USA

    John Samuelson & Phillips W. Robbins

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  1. John Samuelson
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  2. Phillips W. Robbins
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Editors and Affiliations

  1. Laboratory of Biochemistry and Molecular Biology, School of Medicine, Catholic University of Córdoba, Córdoba, Argentina

    Hugo D. Luján PhD

  2. Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden

    Staffan Svärd PhD

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Samuelson, J., Robbins, P.W. (2011). The Glycoproteins of Giardia . In: Luján, H.D., Svärd, S. (eds) Giardia. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0198-8_6

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