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Elektrophorese-Techniken

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Instrumentelle Bioanalytik

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Zusammenfassung

Die „klassische“ Elektrophorese [1–4] ist die Standardmethode der biochemischen und biomedizinischen Analytik zur Trennung und Isolierung von Nucleinsäuren, Aminosäuren, Kohlenhydraten, Peptiden, Proteinen, Enzymen und Glycoproteinen. Es können sowohl anionische und kationische, niedermolekulare Biosubstanzen und große Biopolymere als auch Partikel und Zellen mit Hilfe der Elektrophorese getrennt werden.

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Literaturverzeichnis

  1. A. Chrambach: The practice of quantitative gel electrophoresis, VCH-Verlag, Weinheim (1985)

    Google Scholar 

  2. A. T. Andrews: Electrophoresis, theory techniques and biochemical and clinical applications, Clarendon Press, Oxford (1986)

    Google Scholar 

  3. H. Wagner, R. Kuhn, S. Hofstetter, In: H. Wagner, E. Blasius (Hrsg.): Praxis der elektrophoretischen Trennung, Springer-Verlag, Heidelberg (1989)

    Chapter  Google Scholar 

  4. R. Westermeier: Elektrophorese-Praktikum, VCH-Verlag, Weinheim (1990)

    Google Scholar 

  5. A. Tiselius, Trans Faraday Soc. 33 (1937) 534

    Article  Google Scholar 

  6. K. Hannig, Electrophoresis 3 (1982) 235

    Article  Google Scholar 

  7. C. B. Laurell, Anal. Biochem. 15 (1966) 45

    Article  Google Scholar 

  8. L. Ornstein, Ann. NY Acad Sci. 121 (1964) 321

    Article  Google Scholar 

  9. B. J. Davis, Ann. NY Acad. Sci. 121 (1964) 404

    Article  Google Scholar 

  10. R. H. Maurer. Disk-Elektrophorese-Theorie und Praxis der diskontinuierlichen Polyacrylamid-Elektrophorese, Walter de Gruyter, Berlin (1968)

    Google Scholar 

  11. F. Sänger, A. R. Coulson, J. Mol. Biol. 94 (1975) 441

    Article  Google Scholar 

  12. S. Raymond, L. Weintraub, Science 130 (1959) 711

    Article  Google Scholar 

  13. A. L. Shapiro, E. Viñuela, J. V. Maizel, Biochem. Biophys. Res. Commun. 28 (1967) 815

    Article  Google Scholar 

  14. H. Schägger, G. von Jagow, Anal. Biochem. 166 (1987) 368

    Article  Google Scholar 

  15. F. M. Everaerts, J. M. Becker, Th. P. E. M. Verheggen: Isotachophoresis, theory, instrumentation and applications, J. Chromatogr. Library Vol. 6, Elsevier, Amsterdam (1976)

    Google Scholar 

  16. B. J. Raola, Biochim. Biophys. Acta 295 (1973) 412

    Article  Google Scholar 

  17. A. Görg, W. Postel, R. Westermeier, Anal. Biochem. 89 (1978) 60

    Article  Google Scholar 

  18. P. G. Righetti, In: T. S. Works, R.H. Burdon (Eds.): Isoelectric focusing: theory, methodology and applications, Elsevier Biomedical Press, Amsterdam (1983)

    Google Scholar 

  19. J. W. Jorgenson, K. D. Lukacs, Anal. Chem. 53 (1981) 1298

    Article  Google Scholar 

  20. J. W. Jorgenson, K. D. Lukacs, J. Chromatogr. 218 (1981) 209

    Article  Google Scholar 

  21. S. Hjertén, J. Chromatogr. 270 (1983) 1

    Article  Google Scholar 

  22. S. F. Y. Li: Capillary electrophoresis: principles, practice, and applications, Journal of chromatography library, Vol 52, Elsevier, Amsterdam (1992)

    Google Scholar 

  23. R. Kuhn, S. Hoffstetter-Kuhn: Capillary electrophoresis: principle and practice, Springer-Verlag, Berlin (1993)

    Book  Google Scholar 

  24. F. Foret, L. Krivánková, P. Bocek: Capillary Zone Electrophoresis, VCH-Verlag, Weinheim (1993)

    Google Scholar 

  25. H. Engelhardt, W. Beck, T. Schmitt: Kapillarelektrophorese, Friedr. Vieweg & Sohn Verlag, Braunschweig (1994)

    Book  Google Scholar 

  26. T. Tsuda, K. Nomura, G. Nakagawa, J. Chromatogr. 264 (1983) 385

    Article  Google Scholar 

  27. S. Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 113

    Article  Google Scholar 

  28. D. J. Rose, J. W. Jorgenson, Anal. Chem. 60 (1988) 642

    Article  Google Scholar 

  29. S. Honda, S. Iwase, S. Fujiwara, J. Chromatogr. 404 (1987) 313

    Article  Google Scholar 

  30. J. W. Jorgenson, K. D. Lukacs, Clin. Chem. 27 (1981) 209

    Google Scholar 

  31. G. Schomburg, Trends Anal. Chem. 10 (1991) 163

    Article  MathSciNet  Google Scholar 

  32. K. Turner, LC-GC 9 (1991) 350

    Google Scholar 

  33. Y. Walbroehl, J. W. Jorgenson, J. Chromatogr. 315 (1984) 135

    Article  Google Scholar 

  34. J. S. Green, J. W. Jorgenson, J. Liq. Chromatogr. 12 (1989) 2527

    Google Scholar 

  35. C. P. Ong, C. L. Ng, H. K. Lee, S. F. Y. Li, J. Chromatogr. 547 (1991) 419

    Article  Google Scholar 

  36. M. Goto, K. Irino, D. Ishii, J. Chromatogr. 346 (1985) 167

    Article  Google Scholar 

  37. J. S. Green, J. W. Jorgenson, J. Chromatogr. 352 (1986) 337

    Article  Google Scholar 

  38. L. A. Knecht, E. J. Guthrie, J. W. Jorgenson, Anal. Chem. 56 (1984) 479

    Article  Google Scholar 

  39. I. H. Grant, W. Steuer, J. Microcol. Sep. 2 (1990) 74

    Article  Google Scholar 

  40. R. D. Smith, H. R. Udseth, Nature 331 (1988) 639

    Article  Google Scholar 

  41. J. Romano, P. Jandik, W. R. Jones, P. E. Jackson, J. Chromatogr. 546 (1991) 411

    Article  Google Scholar 

  42. S. Hjertén, Chromatogr. Rev. 9 (1967) 122

    Article  Google Scholar 

  43. K. Altria, C. Simpson, Anal. Proc. 23 (1986) 453

    Google Scholar 

  44. S. Hjerten, M. D. Zhu, J. Chromatogr. 327 (1985) 157

    Article  Google Scholar 

  45. A. S. Cohn, B. L. Karger, J. Chromatogr. 397 (1987) 409

    Article  Google Scholar 

  46. A. S. Cohn, A. Paulus, B. L. Karger, Chromatographia 24 (1987) 14

    Google Scholar 

  47. S. Hjerten, M. D. Zhu, J. Chromatogr. 346 (1985) 265

    Article  Google Scholar 

  48. S. Hjerten, J. L. Liao, K. Yao, J. Chromatogr. 387 (1987) 127

    Article  Google Scholar 

  49. A. Görg, J. S. Fawcett, A. Chrambach, Adv. Electrophoresis 2 (1988) 1

    Google Scholar 

  50. P. G. Righetti: Immobilized pH gradients: Theory and methodology, Elsevier, Amsterdam (1990)

    Google Scholar 

  51. J. R. Mazzeo, I. S. Krull, Bio Techniques 10 (1991) 638

    Google Scholar 

  52. P. Bocek, M. Deml, P. Gebauer, V. Dolnik: Analytical isotachophoresis, VCH-Verlag, Weinheim (1988)

    Google Scholar 

  53. F. M. Everaerts, P. E. M. Verheggen, In: New directions in electrophoretic methods, J. W. Jorgenson, M. Phillips, Amer. Chem. Soc. Symp. Vol. 335, Washington DC (1987) Chap. 4

    Google Scholar 

  54. S. Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111

    Article  Google Scholar 

  55. S. Terabe, K. Otsuka, T. Ando, Anal. Chem. 57 (1985) 834

    Article  Google Scholar 

  56. H. Nishi, S. Terabe, Electrophoresis 11 (1990) 691

    Article  Google Scholar 

  57. S. Terabe, K. Otsuka, T. Ando, Anal. Chem. 61 (1989) 251

    Article  Google Scholar 

  58. K. Otsuka, S. Terabe, J. Microcol. Sep. 1 (1989) 150

    Article  Google Scholar 

  59. V. Pretorius, B. J. Hopkins, J. D. Schieke, J. Chromatogr. 99 (1974) 23

    Article  Google Scholar 

  60. T. Eimer, K. K. Unger, T. Tsuda, Fresenius J. Anal. Chem. 352 (1995) 649

    Article  Google Scholar 

  61. M. M. Dittmann, G. P. Rozing, J. Chromatogr. 744 (1996) 63

    Article  Google Scholar 

  62. U. Pyell, H. Rebscher, A. Banholczer, J. Chromatogr. 779 (1997) 155

    Article  Google Scholar 

  63. A. Maruska, U. Pyell, J. Chromatogr. 782 (1997) 167

    Article  Google Scholar 

  64. J. H. Knox, I. H. Grant, Chromatographia 24 (1987) 135

    Article  Google Scholar 

  65. T. Tsuda, Anal. Chem. 60 (1988) 1677

    Article  Google Scholar 

  66. B. Behnke, E. Bayer, J. Chromatogr. 680 (1994) 93

    Article  Google Scholar 

  67. N. W: Smith, M. B. Evans, Chromatographia 38 (1994) 649

    Article  Google Scholar 

  68. C. Yan, D. Schaufelberger, F. Erni, J. Chromatogr. 670 (1994) 15

    Article  Google Scholar 

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Authors and Affiliations

  1. Institut für Biochemie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Straße 3, 06120, Halle/Saale, Deutschland

    Priv.-Doz. Dr. Manfred H. Gey

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  1. Priv.-Doz. Dr. Manfred H. Gey
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© 1998 Springer Fachmedien Wiesbaden

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Gey, M.H. (1998). Elektrophorese-Techniken. In: Instrumentelle Bioanalytik. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-663-10368-4_5

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  • DOI: https://doi.org/10.1007/978-3-663-10368-4_5

  • Publisher Name: Vieweg+Teubner Verlag, Wiesbaden

  • Print ISBN: 978-3-663-10369-1

  • Online ISBN: 978-3-663-10368-4

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