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Die Detektion von Proteinen auf Membranen: Western-Blot- und Dot-Blot-Verfahren

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Zusammenfassung

Das Western-Blot-Verfahren setzt sich aus insgesamt vier Schritten zusammen: Optimale Probenvorbereitung, Trennung der Proteine durch Gelelektrophorese, Übertragung des Proteinbandenmusters vom Gel auf eine Membran und spezifische Detektion des Antigens. Dieses Kapitel führt in die Technik der Gelelektrophorese und der nachfolgenden Analytik über Western-Blot- und Dot-Blot-Verfahren ein.

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Correspondence to Thorsten Kuczius .

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© 2023 Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature

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Kuczius, T. (2023). Die Detektion von Proteinen auf Membranen: Western-Blot- und Dot-Blot-Verfahren. In: Raem, A.M., Rauch, P. (eds) Immunoassays. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-62671-9_8

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