Zusammenfassung
Zellen im „Reagenzglas“, also in vitro, zu kultivieren, lässt sich grundsätzlich auf zwei verschiedene Arten und Weisen realisieren: zum einen auf einer Oberfläche, an der die Zellen anwachsen, proliferieren und bei Bedarf wieder abgelöst werden können, zum anderen als Suspensionskultur, also schwimmend in einem Nährmedium, ohne direkte Anhaftung an eine Oberfläche. Als 3D-Zellkulturen bezeichnet man sie dann, wenn Zellverbände in mehreren Lagen bzw. als Zellcluster vorliegen, die eine direkte Interaktion zwischen gleichen oder verschiedenen Zelltypen erlauben. Gegenüber zweidimensionalen Zellkulturen, in denen meist nur einen Zelltyp vorhanden ist, können diese 3D-Kulturen einzelne oder mehrere Aspekte der physiologischen Gegebenheiten in Lebewesen (in vivo) genauer darstellen.
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Achberger, K., Antkowiak, L., Liebau, S. (2023). 3D-Zell- und Organoidkultur. In: Raem, A.M., Rauch, P. (eds) Immunoassays. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-62671-9_21
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