Skip to main content
SpringerLink
Log in

Kontaminationen in der Zellkultur

  • Chapter
  • First Online:
Der Experimentator: Zellkultur

Part of the book series: Experimentator ((EXPERIMENTATOR))

  • 8678 Accesses

Zusammenfassung

Obwohl jedes Labor, das mit Zellkulturen experimentiert, ganz sicher schon die eine oder andere unangenehme Erfahrung mit Kontaminationen gemacht hat, wird dieses Problem immer noch unterschätzt. Das hängt mit der Unwissenheit über Ursachen, Bedeutung und Konsequenzen der jeweiligen Kontamination zusammen.Thema dieses Kapitels sind daher alle Arten von mikrobiologischen Kontaminationen die durch Einschleppen diverser Mikroorganismen in die Zellkultur gelangen können. Darunter fallen nicht nur grampositive wie gramnegative Bakterien aller Art, sondern auch bakterielle L-Formen und die gefürchteten Mycoplasmen sowie Pilze einschließlich Hefen und nicht zuletzt auch zahlreiche Viren. Das ist aber noch nicht alles, denn auch Kreuzkontaminationen und falsch identifizierte Zelllinien machen dem Zellkultur-Experimentator das Leben schwer.

O Grausen! Grausen! Grausen! Zung’ und Herz, Fasst es nicht, nennt es nicht!

Aus: Macbeth

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 29.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 39.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Literatur

  1. Rottem S, Naot Y (1998) Subversion and exploitation of host cells by Mycoplasmas. Trends Microbiol 6:436–440

    Article  CAS  Google Scholar 

  2. Lacroix J, Leuchs B, Li J, Hristov G, Deubzer HE, Kulozik AE, Rommelaere J, Schlehofer JR, Witt O (2010) Parvovirus H1 selectively induces cytotoxic effects on human neuroblastoma cells. Int J Cancer 127:1230–1239

    Article  CAS  Google Scholar 

  3. Borovsky Z, Tarshis M, Zhang P, Rottem S (1998) Protein kinase C activation and vacuolation in HeLa cells invaded by Mycoplasma penetrans. J Med Microbiol 47:915–922

    Article  CAS  Google Scholar 

  4. Salman M, Borovsky Z, Rottem S (1998) Mycoplasma penetrans infection of Molt-3 lymphocytes induces changes in the lipid composition of host cells. Microbiology 144(Pt 12):3447–3454

    Article  CAS  Google Scholar 

  5. Rottem S (2003) Interaction of Mycoplasmas with host cells. Physiol Rev 83:417–432

    Article  CAS  Google Scholar 

  6. Ron M, Jorgensen P, Moran U, Weber G, Springer M (2010) BioNumbers – the database of key numbers in molecular and cell biology. Nucleic Acids Res 38:D750–D753

    Article  Google Scholar 

  7. Beran V, Havelkova M, Kaustova J, Dvorska J, Pavlik I (2006) Cell wall deficient forms of Mykobacteria: a review. Veterinarni Medicina 51:365–389

    Article  CAS  Google Scholar 

  8. Martel J, Young JD (2008) Purported nanobacteria in human blood as calcium carbonate nanoparticles. Proc Natl Acad Sci USA 105:5549–5554

    Article  CAS  Google Scholar 

  9. Colcher D, Heberling RL, Kalter SS, Schlom J (1977) Squirrel monkey retrovirus: an endogenous virus of a new world primate. J Virol 23:294–301

    Article  CAS  Google Scholar 

  10. Stang A, Petrasch-Parwez E, Brandt S, Dermietzel R, Meyer HE, Stuhler K, Liffers ST, Uberla K, Grunwald T (2009) Unintended spread of a biosafety level 2 recombinant retrovirus. Retrovirology 6:86

    Article  Google Scholar 

  11. Zentrale Kommission für die Biologische Sicherheit, ZKBS, Stellungnahme der ZKBS zur Risikobewertung des Squirrel Monkey Retrovirus gemäß § 5 Absatz 1 GenTSV (2008)

    Google Scholar 

  12. Buehring GC, Eby EA, Eby MJ (2004) Cell line cross-contamination: how aware are Mammalian cell culturists of the problem and how to monitor it? Vitro Cell Dev Biol Anim 40:211–215

    Article  Google Scholar 

  13. Routray I, Mahmood A, Ngwa NE, Tasleem M, Sahin K, Kucuk O, Shakir A (2016) Cell line cross-contamination and accidental co-culture. J Stem Cell Res Ther. 1:179–185

    Google Scholar 

  14. Nelson-Rees WA, Daniels DW, Flandermeyer RR (1981) Cross-contamination of cells in culture. Science 212:446–452

    Article  CAS  Google Scholar 

  15. Kniss DA, Summerfield TL (2014) Discovery of HeLa cell contamination in HES cells: Call for cell line authentication in reproductive biology research. Reprod Sci 21:1015–1019

    Article  Google Scholar 

  16. Horbach S, Halffman W (2017) The ghosts of HeLa: how cell line misidentification contaminates the scientific literature. PLoS ONE 12:e0186281

    Article  Google Scholar 

  17. Rae JM, Creighton CJ, Meck JM, Haddad BR, Johnson MD (2007) MDA-MB-435 cells are derived from M14 melanoma cells – a loss for breast cancer, but a boon for melanoma research. Breast Cancer Res Treat 104:13–19

    Article  Google Scholar 

  18. Lacroix M (2008) Persistent use of “false” cell lines. Int J Cancer 122:1–4

    Article  CAS  Google Scholar 

  19. Freshney RI (2010) Database of misidentified cell lines. J Int Cancer 126:302

    Article  CAS  Google Scholar 

  20. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (2010) Check your cultures! A list of cross-contaminated or misidentified cell lines. Journal international du cancer 127:1–8

    Article  CAS  Google Scholar 

  21. Torsvik A, Rosland GV, Svendsen A, Molven A, Immervoll H, McCormack E, Lonning PE, Primon M, Sobala E, Tonn JC, Goldbrunner R, Schichor C, Mysliwietz J, Lah TT, Motaln H, Knappskog S, Bjerkvig R (2010) Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track – letter. Can Res 70:6393–6396

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Forschungszentrum Jülich, Jülich, Deutschland

    Sabine Schmitz

Authors
  1. Sabine Schmitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sabine Schmitz .

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Schmitz, S. (2020). Kontaminationen in der Zellkultur. In: Der Experimentator: Zellkultur. Experimentator. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-58951-9_11

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-58951-9_11

  • Published:

  • Publisher Name: Springer Spektrum, Berlin, Heidelberg

  • Print ISBN: 978-3-662-58950-2

  • Online ISBN: 978-3-662-58951-9

  • eBook Packages: Life Science and Basic Disciplines (German Language)

Publish with us

Policies and ethics

Search

Navigation