In Vivo Near-Infrared Fluorescence Imaging Based on Polymer Dots

  • Yixiao Guo
  • Liqin XiongEmail author


Conjugated polymer dots emerge as attractive molecular imaging nanoprobes in living animals because of their excellent optical properties including bright fluorescence intensity, excellent photostability, high emission rates, and low intrinsic cytotoxicity. In this chapter we focused on the preparation of near-infrared (NIR)-emitting polymer dots by nano-precipitation method (matrix-encapsulation method), miniemulsion method, and in situ colloidal Knoevenagel polymerization methods and their applications for in vivo NIR fluorescence imaging, including imaging of lymphatic basins, tumors, zebrafish, and oxygen.

List of Abbreviations




Phthalocyanine- and boron dipyrrin


Bioluminescence resonance energy transfer


Bovine serum albumin


Bisthienylethene derivative, 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)-3,3,4,4,5,5-hexafluoro-1-cyclopentene




BTTP copolymized with 2,7-bis(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9,9-bis[30-(dimethylamino)propyl] fluorine


Homemade heptylamine modified folate


Cyanosubstituted derivatives of poly(p-phenylenevinylene)


CP-loaded DSPE-PEG-folic acid nanoparticles


Cyclic RGD peptides


Anovinylene-backboned conjugated polymers


Cyanovinylene-backboned polymer dots










1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]


1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]




Enhanced permeability and retention


Fluorescence resonance energy transfer




Dual-color SPN-based NIR nanoprobe for the detection of RONS


Silicon 2, 3-naphthalocyanine bis(trihexylsilyloxide)






PS-PEG-COOH modified MEH-PPV polymer dots


PSMA modified MEH-PPV polymer dots


PS-PEG-COOH modified and Luc-8 conjugated MEH-PPV polymer dots


PS-PEG-COOH modified and RGD conjugated MEH-PPV polymer dots


PS-PEG-COOH modified, RGD and Luc-8 conjugated MEH-PPV polymer dots


poly[5-octyl-1-(5-(4,4,9,9-tetrakis(4-hexylphenyl)-4,9-dihydro-s-indaceno[1,2-b:5,6-b0]dithiophen-2-yl)thiophen-2-yl)-3-(thiophen-2-yl)-4H–thieno[3,4-c]pyrrole-4, 6(5H)-dione]


Methoxypolyethylene glycol amine 2000


poly(9,9-dioctylfluorene-alt-benzothiadiazole) average Mn 10,000–20,000








poly(lactic-co-glycolic acid)-poly(ethylene glycol)-folate




poly(styrene-alt-maleic anhydride)


Polystyrene-graft-ethyleneoxide functionalized with carboxyl groups


Platinum(II) meso-bis(pentafluorophenyl)bis-(4-bromophenyl)porphyrin


Reactive oxygen and nitrogen species


Reactive oxygen species


Semiconducting polymer nanoparticles




Tetraethyl orthosilicate






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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research InstituteShanghai Jiao Tong UniversityShanghaiChina

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