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Fetale Zellen und zellfreie Nukleinsäuren im maternalen Blut: Genetische und immunologische Aspekte

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Zusammenfassung

Der Modus operandi der pränatalen Diagnostik ist vor kurzem komplett revolutioniert worden. Ausgehend von den traditionellen invasiven Methoden wie Chorionzottenbiopsie und Amniozentese führte der Weg hin zur Analyse von zellfreien Nukleinsäuren in Proben maternalen Blutes. Der Weg hin zu dieser Revolution war gewunden und griff viele historische Beobachtungen auf, einige davon mehr als 100 Jahre alt. Gleichzeitig baute diese Revolution auf den neuesten Technologien der postgenomischen Entwicklungen auf, um erfolgreich implementiert zu werden. Ein häufig vergessener Mythos besagt, dass die zugrunde liegende Biologie mit einer großen Zahl an Zuständen verbunden ist, die von Präeklampsie bis zur postpartalen Autoimmunerkrankung reichen. In diesem Kapitel geben wir einen Überblick über beide Aspekte, wobei wir zudem eine Chronologie der Ereignisse darstellen, die zur aktuellen Entwicklung geführt haben.

Freundlicherweise aus dem Englischen übersetzt von Prof. B. Huppertz, Graz

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Literatur

  • Bianchi DW, Wilkins-Haug L (2014) Integration of noninvasive DNA testing for aneuploidy into prenatal care: what has happened since the rubber met the road? Clin Chem 60:78–87

    Article  CAS  Google Scholar 

  • Burton GJ, Fowden AL (2015) The placenta: a multifaceted, transient organ. Philos Trans R Soc Lond B Biol Sci 370:20140066

    Article  Google Scholar 

  • Burton GJ, Jauniaux E (2015) What is the placenta? Am J Obstet Gynecol 213: S6–e1, S6–8

    Google Scholar 

  • Conka J, Konecna B, Laukova L, Vlkova B, Celec P (2017) Fetal DNA does not induce preeclampsia-like symptoms when delivered in late pregnancy in the mouse. Placenta 52:100–105

    Article  CAS  Google Scholar 

  • Cronqvist T, Tannetta D, Morgelin M, Belting M, Sargent I et al (2017) Syncytiotrophoblast derived extracellular vesicles transfer functional placental miRNAs to primary human endothelial cells. Sci Rep 7:4558

    Article  Google Scholar 

  • Delorme-Axford E, Donker RB, Mouillet JF, Chu T, Bayer A et al (2013) Human placental trophoblasts confer viral resistance to recipient cells. Proc Natl Acad Sci U S A 110:12048–12053

    Article  CAS  Google Scholar 

  • Drewlo S, Armant DR (2017) Quo vadis, trophoblast? Exploring the new ways of an old cell lineage. Placenta. https://doi.org/10.1016/j.placenta.2017.04.021

    Article  PubMed  Google Scholar 

  • Dumont TMF, Mouillet JF, Bayer A, Gardner CL, Klimstra WB et al (2017) The expression level of C19MC miRNAs in early pregnancy and in response to viral infection. Placenta 53:23–29

    Article  CAS  Google Scholar 

  • Hahn S, Huppertz B, Holzgreve W (2005) Fetal cells and cell free fetal nucleic acids in maternal blood: new tools to study abnormal placentation? Placenta 26:515–526

    Article  CAS  Google Scholar 

  • Hahn S, Lapaire O, Tercanli S, Kolla V, Hosli I (2011) Determination of fetal chromosome aberrations from fetal DNA in maternal blood: has the challenge finally been met? Expert Rev Mol Med 13:e16

    Article  Google Scholar 

  • Hahn S, Hosli I, Lapaire O (2012a) Non-invasive prenatal diagnostics using next generation sequencing: technical, legal and social challenges. Expert Opin Med Diagn 6:517–528

    Article  Google Scholar 

  • Hahn S, Giaglis S, Hoesli I, Hasler P (2012b) Neutrophil NETs in reproduction: from infertility to preeclampsia and the possibility of fetal loss. Front Immunol 3:362

    Article  Google Scholar 

  • Hahn S, Giaglis S, Buser A, Hoesli I, Lapaire O et al (2014) Cell-free nucleic acids in (maternal) blood: any relevance to (reproductive) immunologists? J Reprod Immunol 104–105:26–31

    Article  Google Scholar 

  • Holzgreve W, Hahn S (2000) Fetal cells in cervical mucus and maternal blood. Baillieres Best Pract Res Clin Obstet Gynaecol 14:709–722

    Article  CAS  Google Scholar 

  • Hui L, Bianchi DW (2017) Noninvasive Prenatal DNA Testing: the Vanguard of Genomic Medicine. Annu Rev Med 68:459–472

    Article  CAS  Google Scholar 

  • Lapaire O, Holzgreve W, Oosterwijk JC, Brinkhaus R, Bianchi DW (2007) Georg Schmorl on trophoblasts in the maternal circulation. Placenta 28:1–5

    Article  CAS  Google Scholar 

  • Lo YM (2015) Noninvasive prenatal diagnosis: from dream to reality. Clin Chem 61:32–37

    Article  CAS  Google Scholar 

  • Morales-Prieto DM, Ospina-Prieto S, Chaiwangyen W, Schoenleben M, Markert UR (2013) Pregnancy-associated miRNA-clusters. J Reprod Immunol 97:51–61

    Article  CAS  Google Scholar 

  • Moser G, Gauster M, Orendi K, Glasner A, Theuerkauf R et al (2010) Endoglandular trophoblast, an alternative route of trophoblast invasion? Analysis with novel confrontation co-culture models. Hum Reprod 25:1127–1136

    Article  CAS  Google Scholar 

  • Moser G, Weiss G, Sundl M, Gauster M, Siwetz M et al (2017) Extravillous trophoblasts invade more than uterine arteries: evidence for the invasion of uterine veins. Histochem Cell Biol 147:353–366

    Article  CAS  Google Scholar 

  • Nelson JL (2012) The otherness of self: microchimerism in health and disease. Trends Immunol 33:421–427

    Article  CAS  Google Scholar 

  • Nelson JL, Furst DE, Maloney S, Gooley T, Evans PC et al (1998) Microchimerism and HLA-compatible relationships of pregnancy in scleroderma. Lancet 351:559–562

    Article  CAS  Google Scholar 

  • Ostensen M, Brucato A, Carp H, Chambers C, Dolhain RJ et al (2011) Pregnancy and reproduction in autoimmune rheumatic diseases. Rheumatology (Oxford) 50:657–664

    Article  Google Scholar 

  • Phillippe M (2014) Cell-free fetal DNA–a trigger for parturition. N Engl J Med 370:2534–2536

    Article  Google Scholar 

  • Rather IA, Lone JB, Bajpai VK, Paek WK, Lim J (2017) Zika virus: an emerging worldwide threat. Front Microbiol 8:1417

    Article  Google Scholar 

  • Romero R, Dey SK, Fisher SJ (2014) Preterm labor: one syndrome, many causes. Science 345:760–765

    Article  CAS  Google Scholar 

  • Scharfe-Nugent A, Corr SC, Carpenter SB, Keogh L, Doyle B et al (2012) TLR9 provokes inflammation in response to fetal DNA: mechanism for fetal loss in preterm birth and preeclampsia. J Immunol 188:5706–5712

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Universitätsspital Basel, Frauenklinik, Basel, Schweiz

    Olav Lapaire

  2. Departement Biomedizin, Labor für Pränatalmedizin, Universitätsspital Basel/Universität Basel, Basel, Schweiz

    Shane Vontelin van Breda, Simona Rossi & Sinuhe Hahn

  3. Faculty of Medicine, Institute of Molecular Biomedicine, Comenius University Bratislava, Bratislava, Slovakia

    Lenka Vokalova & Peter Celec

  4. Klinik für Geburtshilfe und Schwangerschaftsmedizin, Universitätsspital Basel, Basel, Schweiz

    Irene Hösli

Authors
  1. Olav Lapaire
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  2. Shane Vontelin van Breda
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  3. Lenka Vokalova
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  4. Peter Celec
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  5. Irene Hösli
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  6. Simona Rossi
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  7. Sinuhe Hahn
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Correspondence to Olav Lapaire .

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Editors and Affiliations

  1. Ordinarius, Lehrstuhl für Zellbiologie Histologie und Embryologie, Gottfried Schatz Forschungszentrum, Medizinische Universität Graz, Graz, Austria

    Berthold Huppertz

  2. Klinik für Frauenheilkunde und Geburtshilfe, Universitätsklinikum Jena, Jena, Germany

    Ekkehard Schleußner

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Lapaire, O. et al. (2018). Fetale Zellen und zellfreie Nukleinsäuren im maternalen Blut: Genetische und immunologische Aspekte. In: Huppertz, B., Schleußner, E. (eds) Die Plazenta. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-55622-1_14

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  • DOI: https://doi.org/10.1007/978-3-662-55622-1_14

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-55621-4

  • Online ISBN: 978-3-662-55622-1

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