Collection, preservation and culturing
Macroscopic freshwater ulvophytes, including Cladophora and Ulva species, are easily observable and collectable. For these species, thalli can be preserved as dried herbarium specimens, or as wet specimens in 5 % formalin solution or 95 % ethanol. For molecular characterization, clean portions of the thallus (e. g. apical tips or young thallus parts) can be dried in silica-gel, or preserved in 95 % ethanol. Unialgal cultures of these larger ulvophytes can be prepared by incubating clean, young apical portions of the thallus into freshwater culture medium, or by isolation of zoospores.