Background History of the National and International Brassica rapa Genome Sequencing Initiatives
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Whole genome sequencing of Brassica rapa was first launched by the multinational group of Brassica rapa Genome Sequencing Project (BrGSP). The group planned to perform the assembly using the method called “bacterial artificial chromosome (BAC) by BAC” in the initial stage. However, the progress was limited and only chromosome A03 was finished under this method. Along with the development of the second generation sequencing technology, the Chinese suggest to adopt this new sequencing method and initiative assembled the B. rapa genome in short time by SOAP-denovo, which integrated the data of pair-ends short reads generated from the Illumina sequencing platform and the data of BAC sequences from BrGSP. This well assembled whole genome sequences of B. rapa—verified by the comparison to the A03 assembled by BAC sequenced—was then serves as the genome reference for the evolution, gene mapping and function studies of B. rapa.
KeywordsBacterial Artificial Chromosome Next Generation Sequencing Bacterial Artificial Chromosome Clone Bacterial Artificial Chromosome Library Next Generation Sequencing Data
- Tang H, Woodhouse MR, Cheng F, Schnable JC, Pedersen BS et al (2012) Altered patterns of fractionation and exon deletions in Brassica rapa support a two-step model of paleohexaploidy. Genetics 190, 1563–1574.Google Scholar