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Introduction to Aptamer and Cell-SELEX

  • Libo Zhao
  • Weihong Tan
  • Xiaohong FangEmail author
Chapter

Abstract

Aptamers are functional oligonucleic acids that bind to specific targets with high affinity. They have demonstrated their unique advantages as chemical antibodies in molecular recognition for many applications in biomedicine and biotechnology such as biosensing, bioimaging, and drug. In particular, a recently developed method of live cell-based in vitro selection, termed cell-SELEX, has successfully isolated aptamers without prior knowledge of a cell’s molecular signature, making such aptamers promising for applications in molecular medicine. This chapter reviews the evolution of cell-SELEX technology and introduces the content of this book, which surveys the advancements in cell-SELEX DNA aptamer selection, characterization, modification, and application for theranostics.

Keywords

Aptamer DNA/RNA oligonucleotide Antibody Cell-SELEX Theranostics 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Key Laboratory of Molecular Nanostructure and NanotechnologyInstitute of Chemistry, Chinese Academy of SciencesBeijingChina
  2. 2.College of Biology, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular MedicineHunan UniversityChangshaChina
  3. 3.Departments of ChemistryUniversity of FloridaGainesvilleUSA

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