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Advances in Applied Biotechnology pp 453–460Cite as

The Semi-continuous Cultivation of Nostoc flagelliforme Cells

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 332))

Abstract

The semi-continuous cultivation is an important technique for large-scale liquid photoautotrophic cultivation of microalages, in which the excessive cells can be removed and the nutrient elements in the culture medium can be supplemented in time. In this paper, the semi-continuous cultivation of Nostoc flagelliforme cells was conducted and the cultivation conditions were optimised in order to achieve maximum biomass production. After single factor and Box-Behnken experiment with 15 days, the largest biomass amount (0.305 g/l) can be harvested at the best culture conditions (update rate 33 %, update cycle 1 day, and sodium nitrate concentration 1.6 g/l). This study offered important basic data for the implementation of large-scale photoautotrophic cultivation of N. flagelliforme cells.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31271809).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Bioengineering, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Lifang Yue, Yupeng Xiao, Guojuan Sun & Shiru Jia

  2. Fuqing Xin Da Ze Spirulina Cooperation Limited, Fuqing, 350313, People’s Republic of China

    Xing Zheng

  3. Tianjin Key Laboratory of Industrial Microbiology, School of Bioengineering, Tianjin University of Science & Technology, TEDA, 13th Street, Tianjin, 300457, China

    Yujie Dai

Authors
  1. Lifang Yue
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  2. Yupeng Xiao
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  3. Guojuan Sun
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  4. Shiru Jia
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  5. Yujie Dai
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  6. Xing Zheng
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Corresponding author

Correspondence to Yujie Dai .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Yue, L., Xiao, Y., Sun, G., Jia, S., Dai, Y., Zheng, X. (2015). The Semi-continuous Cultivation of Nostoc flagelliforme Cells. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_47

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