Abstract
10-HDA (10-hydroxy-2-decenoic acid) is an unsaturated medium-chain fatty acid, which is the main active component of royal jelly. It has been shown to possess biological activity in antibacterial, immunoregulation, and antitumour. However, the underlying antibacterial mechanism of 10-HDA is unclear. In this study, it is shown that 10-HDA is a broad-spectrum antimicrobial agent and has obvious inhibition effects on multiple pathogenic bacteria with a concentration-dependent mode of inhibitory effect. The minimum inhibitory concentration (MIC) of Bacillus subtilis is 0.62 mg/mL. Furthermore, the acting mechanism of 10-HDA on B. subtilis is investigated by analyzing the DNA binding ability of 10-HDA with gel retardation assay and atomic force microscope. The results indicate that 10-HDA can combine with the bacterial DNA strongly and inhibit migration of DNA on Sepharose gel. By detecting the effect of 10-HDA on DNA content of bacteria, it was shown that 10-HDA had inhibitory effect on DNA synthesis of bacteria. Our results suggest that 10-HDA can combine with bacterial DNA, which further inhibits the DNA synthesis, and thus suppress the growth and activity of bacteria.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (No: 31201281 and 31171727) and the Research award fund for outstanding young scientists of Shandong province (No: BS2013SW029).
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Yang, X., Li, J., Wang, R. (2015). Antibacterial Mechanism of 10-HDA Against Bacillus subtilis . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_34
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DOI: https://doi.org/10.1007/978-3-662-45657-6_34
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