Abstract
Enduracidin is a kind of lipodepsipeptides and composed by a 17 amino acid residual conjugated with a fatty acid strain. It is used as a feed additive approved by EU because of its high safety, low toxicity, low-residue, and activity against a wide variety of Gram-positive bacteria. Here, we combined a physical mutagenesis of 137Cs γ-irradiation with the atmospheric and room temperature plasma (ARTP) biological breeding system to improve enduracidin productivity in the industrial Streptomyces. The mutation rates of spores treated by ARTP for 1 min and by 137Cs γ-irradiation for 500 Gy dosages were 26.51 and 19.41 %, respectively. An optimal process for breeding by the combination was proposed. Through the high throughout screening method, the mutant Streptomyces fungicidicus S-224 was obtained, and its enduracidin yield reached to 1.58 g/L that was 1.65-fold to the original strain. The mutation breeding combined ARTP with 137Cs γ rays was the first trial and provided an alternative method for industrial strains improvement.
Keywords
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Fang X, Tiyanont K, Zhang Y et al (2006) The mechanism of action of ramoplanin and enduracidin. Mol BioSyst 2(1):69–76
Gao YJ, Zhang Y (2010) Peptides feed additive–enduracidin. Feed Rev (4):32–35
Gong GL, Sun X, Liu XL et al (2007) Mutation and a high-throughput screening method for improving the production of epothilones of Sorangium. J Ind Microbiol Biotechnol 34(9):615–623
Guo T, Tang Y, Xi YL et al (2011) Clostridium beijerinckii mutant obtained by atmospheric pressure glow discharge producing high proportions of butanol and solvent yields. Biotechnol Lett 33(12):2379–2383
Hosaka T, Ohnishi-Kameyama M, Muramatsu H et al (2009) Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12. Natl Biotechnol 27:462–464
Irina A, Vsevolod P (2004) Structural basis for transcription regulation by alarmone ppGpp. Cell 117(3):299–310
Jin L, Fang M, Zhang C et al (2011) Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants. Chin J Biotechnol 27(3):461–467
Lerouge S, Tabrizian M, Wertheimer MR et al (2002) Safety of plasma-based sterilization:surface modifications of polymeric medical devices induced by sterrad and plazlyte processes. Biomed Mater Eng 12(1):3–13
Li HF, Gao XJ, Bai LJ et al (2012) Rapid Selection of high yield enramycin. China J Vet Med 46(5):40–42
Lu Y, Wang LY, Ma K et al (2011) Characteristics of hydrogen production of an Enterobacter aerogenes mutant generated by a new atmospheric and room temperature plasma (ARTP). Biochem Eng J 55(1):17–22
Lv XA, Jin YY, Li YD et al (2012) Genome shuffling of Streptomyces viridochromogenes for improved production of avilamycin. Appl Microbiol Biotechnol 97:641–648
Ochi K (2007) From microbial differentiation to ribosome engineering. Biosci Biotechnol Biochem 71(6):1373–1386
Tan YY, Jin LH, Zhang C et al (2012) Mutation of oleaginous yeast using atmospheric and room temperature plasma for xylose utiliability and characteristics of typical mutant. Food Sci 33(1):212–217
Wang LY, Huang ZL, Li G et al (2010) Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma. J Appl Microbiol 108(3):851–858
Wang Q, Feng LR, Wei L et al (2014) Mutation breeding of lycopene-producing strain Blakeslea trispora by a novel atmospheric and room temperature plasma (ARTP). Appl Biochem Biotechnol 174(1):452–460
Wang Q, Zhang D, Li Y et al (2014) Genome shuffling and ribosome engineering of Streptomyces actuosus for high-yield nosiheptide production. Appl Biochem Biotechnol 173(6):1553–1563
Wu T, Huang H, Zhou P (1998) The Inhibitory effects of enduracidin on hepatitis B virus in vitro. Virol Sinica 13(1):46–49
Xia SQ, Liu L, Zhang DX et al (2010) Mutation and selection of transglutaminase producing strain by atmospheric pressure glow discharge plasma. Microbiol China 37(11):1642–1649
Xie SJ, Xiao J, Xu J (2009) Advance in microbial ribosome engineering. Acta Microbiologica Sinica 49(8):981–986
Yin XH, Zabriskie TM (2006) The enduracidin biosynthetic gene cluster from Streptomyces fungicidicus. Microbiol 152(10):2969–2983
Zhang X, Zhang XF, Li HP et al (2014) Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool. Appl Microbiol Biotechnol 98(12):5387–5396
Zhu CH, Lu FP, He YN et al (2007) Regulation of avilamycin biosynthesis in Streptomyces viridochromogenes:effects of glucose, ammonium ion, and inorganic phosphate. Appl Microbiol Biotechnol 73(5):1031–1038
Acknowledgments
We thank the technical support by Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China, and Zhejiang Academy of Agricultural Sciences. The work was financially supported by College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Zhang, D., Wang, Q., Liang, X. (2015). Breeding High Producers of Enduracidin from Streptomyces fungicidicus by Combination of Various Mutation Treatments. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 332. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45657-6_14
Download citation
DOI: https://doi.org/10.1007/978-3-662-45657-6_14
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-45656-9
Online ISBN: 978-3-662-45657-6
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)