Abstract
The filamentous fungi strain Penicillium canescens has been developed as a host for the production of heterologous proteins and enzymes for biorefinery. There are several features of this strain which make it an attractive option as a host expression system. First, P. canescens has a high growth rate and the developed system of biosynthesis of extracellular enzymes; second, strain needs inexpensive fermentation medium using sugar beet pulp as a main substrate; third, the fermentation process can be easily scaled up; and fourth, there is auxotrophic strain P. canescens which can be transformed by plasmid DNA with exogenous genes. All these factors make possible to create new efficient recombinant strains and enzyme preparations (different endo-glucanases and cellobiohydrolases, β-glucosidase, pectin lyase, inulinases) that are in demand by various sectors of industry and biorefinery.
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This study was supported by the Russian Science Foundation (GRANT 14-14-00881).
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Sinitsyn, A.P., Rozhkova, A.M. (2015). Penicillium canescens Host as the Platform for Development of a New Recombinant Strain Producers of Carbohydrases. In: Kamm, B. (eds) Microorganisms in Biorefineries. Microbiology Monographs, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45209-7_1
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DOI: https://doi.org/10.1007/978-3-662-45209-7_1
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