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Induced Genetic Variation, TILLING and NGS-Based Cloning

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Biotechnological Approaches to Barley Improvement

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 69))

Abstract

Mutagenesis is one of the most important tools available to barley geneticists and breeders in order to investigate trait inheritance and to provide useful genetic variation to breeding programmes. Recent advancements in genomics, including the increasing availability of barley genome sequence information, are making mutagenesis even more valuable. In a forward genetics perspective (from traits to genes), the main improvements are being obtained by the exploitation of high-throughput phenotyping and genotyping. SNP genotyping and next-generation sequencing (NGS) platforms enable to genetically and physically map, or even to clone, target mutant genes in single-step experiments, once segregating populations are available. In barley, reverse genetics (from genes to traits), both transposon-based mutagenised populations and multiple TILLING resources, are becoming available or increasing their coverage. These resources too can be made more effective if matched with NGS-based molecular screening.

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Acknowledgments

We thank Iwona Szarejko and Roberto Tuberosa for useful discussions, Brian Forster for suggestions over an earlier version of this manuscript and Riccardo Bovina, Simona Corneti and Valentina Talamè for technical assistance.

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Authors and Affiliations

  1. Department of Agricultural Science, University of Bologna, viale Fanin 44, 44127, Bologna, Italy

    Silvio Salvi & Sara Giulia Milner

  2. The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK

    Arnis Druka

  3. Faculty of Biology and Environment Protection, Department of Genetics, University of Silesia, 40-032, Katowice, Poland

    Damian Gruszka

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  1. Silvio Salvi
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  2. Arnis Druka
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  3. Sara Giulia Milner
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  4. Damian Gruszka
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Correspondence to Silvio Salvi .

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Editors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Jochen Kumlehn

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Nils Stein

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Salvi, S., Druka, A., Milner, S.G., Gruszka, D. (2014). Induced Genetic Variation, TILLING and NGS-Based Cloning. In: Kumlehn, J., Stein, N. (eds) Biotechnological Approaches to Barley Improvement. Biotechnology in Agriculture and Forestry, vol 69. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-44406-1_15

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