Abstract
Following the seminal work on TILLING in Arabidopsis thaliana, a population of EMS-mutagenized plants was established for Lotus japonicus ‘Gifu’ to be used for both forward and reverse screening. This was developed into the Lotus TILLING platform which subsequently became RevGenUK covering not only TILLING in L. japonicus, but also Medicago truncatula and other species. Over the last 10 years, nearly two thousand mutations for more than 160 genes have been identified using the platform. In this article, we cover the history, development and current technology employed in the process and explore the impact TILLING has had on Lotus research.
Keywords
- Conventional Tilling
- Lotus Japonicus
- Sort Intolerant From Tolerant
- Ethylmethane Sulphonate
- Fred Hutchinson Cancer Research
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Wang, T.L., Robson, F. (2014). TILLING in Lotus japonicus . In: Tabata, S., Stougaard, J. (eds) The Lotus japonicus Genome. Compendium of Plant Genomes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-44270-8_21
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DOI: https://doi.org/10.1007/978-3-662-44270-8_21
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