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Application of Enzyme Bioluminescence for Medical Diagnostics

  • Ludmila A. Frank
  • Vasilisa V. Krasitskaya
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 144)

Abstract

Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.

Graphical Abstract

Keywords

Bioluminescence Ca2+-regulated photoprotein Diagnostics Immunoassay Luciferase Nucleic acid hybridization assay 

Abbreviations

Ab

Antibody

Ag

Antigen

ATP

Adenosine-5′-triphosphate

BRET

Bioluminescence resonance energy transfer

CMV

Cytomegalovirus

ELISA

Enzyme-linked immunosorbent assay

EYFP

Yellow fluorescent protein

GFP

Green fluorescent protein

hCG

Human chorionic gonadotropin

hFSH

Follicle stimulating gonadotropic hormone

hGH

Human growth hormone

hGLuc

Highly bright Gaussia luciferase

hLH

Luteinizing gonadotropic hormone (lutropin)

JAK2

Janus kinase 2

LIPS

Luciferase immunoprecipitation system

LNA

Locked nucleic acid

MBL

Mannose-binding lectin

miRNA

Micro RNA

mRNA

Messenger RNA

MTB

Mycobacterium tuberculosis

NASBA

Nucleic acid sequence-based amplification

PAP

Prostatic acid phosphatase

PCR

Polymerase chain reaction

PEXT

Primer extension

PSA

Prostate specific antigen

RLuc

Renilla luciferase

RT-PCR

Reverse transcription polymerase chain reaction

SNP

Single nucleotide polymorphism

TLR4

Toll-like receptor 4

TSH

Thyroid stimulation hormone (thyrotropin)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Photobiology Laboratory, Institute of BiophysicsRussian Academy of SciencesKrasnoyarskRussia
  2. 2.Siberian Federal UniversityKrasnoyarskRussia

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