Summary
X-ray microanalysis was used to compare chemically untreated cryosections of quick-frozen myocardial tissue in “caffeine contracture” with cryosections of normal muscle. Our goal was to find out if it is possible by means of this method to detect changes in the calcium compartmentalization of the myocardial cell occurring by changes in its functional state. While it is possible to quantitate calcium in the cisternae of sarcoplasmic reticulum of the control muscle preparation, calcium could never be detected in these compartments of caffeine-contracted muscles.
In active microsomal fraction of ventricular myocardium it is possible to quantitate calcium and also to distinguish two components on account of their different ability to accumulate this element. The calcium content is different in the two components of the fraction.
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References
Carafoli, E., M. Crompton: In: Calcium transport and cell function. New York Acad. of Science, 269–285 (1978).
Gonzales-Serratos, H., A. V. Somlyo, G. McClennan, H. Shuman, L. Borrero, A. P. Somlyo: Composition of vacuoles and sarcoplasmic reticulum in fatigued muscle: Electron probe analysis. Proc. Nati. Acad. Sci. USA 75, No. 3, 1329–1333 (1978).
Holubarsch, Ch., R. Jacob: Evaluation of elastic properties of myocardium. Experimental models of fibrosis and contracture in heart muscle strips. Z. Kardiol. 68, 123–127 (1979).
Katz, A. M., D. I. Repke, W. Hasselbach: Dependence on Ionophore-and caffeine-induced calcium release from sarcoplasmic reticulum vesicles on external and internal calciumion concentrations. J. Biol. Chemistry] 252, No. 6, 1938–1949 (1977).
Lechene, P.: Electron probe micro analysis, its present — its future. Am. J. Physiol. 232(5), F 391–397 (1977).
Jacob, K., G. Ebrecht, A. Kämmereit, I. Medugorac, M. F. Wendt-Gallitelli: Myocardial function in different models of cardiac hypertrophy. An attempt at correlating mechanical, biochemical and morphological parameters. Basic Res. Cardiol. 72, 160–167 (1977)
Seveus, L., D. Brdiczka, T. Barnard: On the occurrence and composition of dense particles in mitochondria in ultrathin frozen dry sections. Cell. Biol. Int. Rep. 2, 155–162 (1978).
Shuman, H., A. V. Somlyo, A. P. Somlyo: Quantitative electron probe mic-roanalysis of biological thin sections: methods and validity. Ultramicroscopy. 1, 317–339 (1976).
Somlyo, A. V., J. Silcox, A. P. Somlyo: Electron probe analysis and cryo-ultramicrotomy of cardiac muscle: mitochondrial granula. Proc. EMSA Meeting 532–533 (1975).
Wendt-Gallitelli, M. F., H. Wolburg, W. Schlote: Problems in the quantitation of diffusible ions in unstained heart muscle cryosections by X-ray microanalysis.
Dortmunder Gespräche: Crytotechniques for Microanalysis of Biological Objects. Arzneim.-Forsch./Drug Res. 29(II), 11, 1814–1815 (1979).
Wendt-Gallitelli, M. F., H. Wolburg, M. Schwegler, W. Schlote: Electron probe X-ray microanalysis and cryoultramicrotomy of unstained myocardial frag mented and in situ sarcoplasmic reticulum. Experientia 35(12), 1591–1593 (1979).
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© 1980 Springer-Verlag Berlin Heidelberg
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Wendt-Gallitelli, M.F., Wolburg, H., Schlote, W., Schwegler, M., Holubarsch, C., Jacob, R. (1980). Prospects of X-ray microanalysis in the study of pathophysiology of myocardial contraction. In: Jacob, R. (eds) Experimental Cardiac Hypertrophy and Heart Failure. Steinkopff, Heidelberg. https://doi.org/10.1007/978-3-662-41468-2_10
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DOI: https://doi.org/10.1007/978-3-662-41468-2_10
Publisher Name: Steinkopff, Heidelberg
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