Abstract
The Mitchell hypothesis of chemiosmotic coupling in oxidative phosphorylation is examined in the light of experimental data on oxidative phosphorylation at present available. The following objections are brought against the theory:
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1.
Data on the magnitude of the phosphate potential against which ATP can be synthesized by the respiratory chain require, on the basis of chemiosmotic coupling, very effective proton and/or cation extrusion and/or anion uptake by the mitochondria. Experimental evidence for this is lacking. Indeed it has been shown by Chance and Mela that mitochondria in the controlled state do not extrude protons.
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2.
Although reversible ATP-driven proton (chloroplasts) or cation (mitochondria, erythrocytes) pumps have been demonstrated, this is insufficient evidence for the postulate that the establishment of a proton gradient is the primary energy-conserving event of the chloroplast or mitochondrial respiratory chain. Indeed, it is general experience that cations are required for the extrusion of protons by mitochondria.
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3.
The stoicheiometry of proton extrusion by mitochondria and of proton uptake by chloroplasts, and the kinetics of the latter process are also difficult to reconcile with the chemiosmotic hypothesis.
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4.
Further experimental evidence is presented confirming that, under the conditions of the oxygen-pulse experiments of Mitchell and Moyle, the extrusion of H+ is not associated with the oxidation of mitochondrial NADH.
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5.
The respiratory chain included in the chemiosmotic hypothesis is difficult to reconcile with our present knowledge of the chain.
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References
Slater, E. C., Nature, 172 (1953) 975.
Mitchell, P., Nature, 191 (1961) 144.
Mitchell, P., Chemiosmotic coupling in oxidative and photosynthetic phosphorylation, Glynn Research Ltd., Bodwin 1966.
Mitchell, P., Biol. Rev. 41 (1966) 445.
Reid, R. A., Moyle, J., and Mitchell, P., Nature, 212 (1966) 257.
Mitchell, P., and Moyle, J., Nature, 213 (1967) 137.
Robertson, R. N., Biol. Rev. 35 (1960) 231.
Williams, R. J. P., J. Theoret. Biol. 1 (1961) 1.
Williams, R. J. P., J. Theoret. Biol. 3 (1962) 209.
Cockrell, R. S., Harris, E. J., and Pressman, B. C., Biochemistry, 5 (1966) 2326.
Klingenberg, M., and Schollmeyer, P., in Intracellular Respiration: Phosphorylating and Non-Phosphorylating Oxidation Reactions, Proceedings of the Fifth International Congress of Biochemistry, Moscow 1961, Pergamon Press, Oxford 1963, Vol. 5, p. 46.
Jagendorf, A. T., and Hind, G., in Photosynthesis mechanisms in green plants (edited by B. Kok and A. T. Jagendorf ), National Academy of Sciences, Washington 1963, p. 599.
Saris, N. C., Dissertation, University of Helsinki, 1963.
Jagendorf, A. T., and Uribe, E., Proc. Natl. Acad. Sci. U. S. 55 (1966) 170.
Tager, J. M., Veldsema-Currie, R. D., and Slater, E. C., Nature, 212 (1966) 376.
McCarty, R. E., and Racker, E., Federation Proc. 25 (1966) 226.
Chance, B., and Mela, L., Nature, 212 (1966) 369, 372.
Chance, B., and Hollunger, G., J. Biol. Chem. 236 (1961) 1534.
Mayne, B. C., and Clayton, R. K., Proc. Natl. Acad. Sci. U. S. 55 (1966) 494.
Jagendorf, A. T., and Uribe, E., in Brookhaven Symp. Biol. 19: BNL 989 (C-48), 1967, in the press.
Jardetzky, O., Nature, 211 (1966) 969.
Garrahan, P. J., and Glynn, I. M., Nature, 211 (1966) 1414.
Lehninger, A. L., Rossi, C. S., Bielawski, J., and Gear, A., in Mitochondrondrial Structure and Compartmentation (edited by S. Papa, J. M. Tager, E. Quagliariello, and E. C. Slater), Adriatica Editrice, Bari, 1967, in the press.
Mitchell, P., and Moyle, J., Nature, 208 (1965) 147.
Mitchell, P., and Moyle, J., in Biochemistry of Mitochondria (edited by E. C. Slater, Z. Kaniuga, and L. Wojtczak ), Academic Press and P.W.N., London and Warsaw, 1967, p. 53.
Chance, B., and Williams, G. R., Advan. Enzymol. 17 (1956) 65.
Dam, K. van, Biochim. Biophys. Acta, 128 (1966) 337.
Carafoli, E., Gamble, R. L., and Lehninger, A. L., Biochem. Biophys. Res. Commun. 21 (1965) 215.
Rossi, C., and Azzone, G. F., Biochim. Biophys. Acta, 110 (1965) 434.
Rienits, K. G., unpublished observations.
Slater, E. C. (Ed.), Flavins and Flavoproteins (BBA Library, Vol. 8 ), Elsevier, Amsterdam, 1966.
Slater, E. C., Colpa-Boonstra, J. P., and Links, J., in Ciba Foundation Symp. on Quinones in Electron Transport, Churchill, London, 1961, p. 161.
Slater, E. C., in Biochemistry of Mitochondria (edited by E. C. Slater, Z. Kaniuga, and L. Wojtczak ), Academic Press and P.W.N., London and Warsaw, 1967, p. 1.
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Slater, E.C. (1967). An Evaluation of the Mitchell Hypothesis of Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation. In: Liébecq, C. (eds) European Journal of Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-25813-2_43
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