Abstract
Poly-l-lysyl RNase was prepared by the reaction of RNase with ε,N-trifluoroacetyl-α,Ncarboxy-l-lysine anhydride, either in dioxane-aqueous phosphate buffer or in dimethylformamide in the absence of phosphate. The trifluoroacetyl group was subsequently removed from polytrifluoroacetyllysyl RNase by 1.0 M aqueous piperidine. Derivatives prepared in aqueous media and enriched with up to 70 lysine residues, and derivatives prepared in dimethylformamide enriched with up to 270 lysine residues, were soluble and retained some activity. This activity could be completely recovered in all preparations after reduction of the disulfide bonds, followed by reoxidation. Approximately four phenolic hydroxyl groups were found, by spectrophotometric titration, to ionise normally in polylysyl RNase, as opposed to three in the native enzyme.
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References
Herriot, R. M., Advan. Protein Chem. 3 (1947) 170.
Sri Ram, J., Bier, M., and Maurer, P. H., Advan. in Enzymol. 24 (1962) 105.
Becker, R. R., and Stahmann, M. A., J. Biol. Chem. 204 (1953) 745.
Katchalski, E., Sela, M., Silman, H. I., and Berger, A., in The Proteins (edited by H. Neurath ), Acad. Press, New York 1964, Vol. II, p. 405.
Anfinsen, C. B., Sela, M., and Cooke, J. P., J. Biol. Chem. 237 (1962) 1825.
Wellner, D., Silman, H. I., and Sela, M., J. Biol. Chem. 238 (1963) 1324.
Cooke, J. P., Sela, M., and Anfinsen, C. B., J. Biol. Chem. 238 (1963) 2034.
Becker, R. R., in Polyaminoacids, Polypeptides and Proteins (edited by M. A. Stahmann), University of Wisconsin Press, Madison 1962, paper 29.
Epstein, C. J., and Goldberger, R. F., J. Biol. Chem. 239 (1964) 1087.
Becker, R. R., and Sawada, E., Federation Proc. 22 (1963) 419.
Hirs, C. H. W., Moore, S., and Stein, W. H., J. Biol. Chem. 200 (1953) 493.
Bernfield, M. R., J. Biol. Chem. 240 (1965) 4753.
Sela, M., Arnon, R., and Jacobson, I., Biopolymers, 1 (1963) 517.
Kupke, D., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 32 (1961) 107.
Brown, D. M., Magrath, D. I., and Todd, A. R., J. Chem. Soc. (1952) 2708.
Thang, M. N., Graffe, M., and Grunberg-Manago, M., Biochim. Biophys. Acta, 108 (1965) 125.
Hauptschein, M., Stokes, C. S., and Nodiff, E. A., J. Am. Chem. Soc. 74 (1952) 4005.
Anfinsen, C. B., Redfield, R. R., Choate, W. L., Page, J., and Caroll, W. R., J. Biol. Chem. 207 (1954) 201.
Kunitz, M., J. Biol. Chem. 164 (1946) 563.
Richards, F. M., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 29 (1955) 315.
Warner, R. C., J. Biol. Chem. 229 (1957) 717.
Kay, R. E., Harris, D. C., and Entenman, C., Arch. Biochem. Biophys. 63 (1956) 14.
Eger, C., and Yarden, A., Anal. Chem. 28 (1956) 512.
Boyer, P. D., J. Am. Chem. Soc. 76 (1954) 4331.
Ellman, G. L., Arch. Biochem. Biophys. 82 (1959) 70.
Spackman, D. H., Stein, W. H., and Moore, S., Anal. Chem. 30 (1958) 1190.
Yphantis, D. A., Ann. N. Y. Acad. Sci. 88 (1960) 586.
Davies, B. J., Ann. N. Y. Acad. Sci. 121 (1964) 404.
Anfinsen, C. B., and Haber, E., J. Biol. Chem. 236 (1960) 1361.
Epstein, C. J., Goldberger, R. F., Young, D. M., and Anfinsen, C. B., Arch. Biochem. Biophys. Supp. 1 (1962) 223.
Shapira, R., Biochem. Biophys. Res. Commun. 1 (1959) 236.
Hummel, J. P., and Anderson, B. S., Arch. Biochem. Biophys. 112 (1965) 443.
Goldberger, R. F., and Anfinsen, C. B., Biochemistry, 1 (1962) 401.
Hirs, C. H. W., Haimann, M., and Kycia, J. H., in Biological Structure and Function (edited by T. W. Goodwin and O. Lindberg), Academic Press, New York 1961, Vol. I.
Scheraga, H. A., and Rupley, J. A., Advan. Enzymol. 24 (1962) 161.
Harrington, W. F., and Schellman, J. A., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 30 (1956) 21.
Applequist, J. B., Ph. D. Thesis, Harvard University (1959).
Krausz, L. M., Scaduto, F. C., Sawada, F., and Becker, R. R., Federation Proc. 21 (1962) 405.
Kettman, M. S., Nishikawa, A. H., Morita, R. Y., and Becker, R. R., Biochem. Biophys. Res. Commun. 22 (1966) 262.
Shugar, D., Biochem. J. 52 (1952) 142.
Tanford, C., Hauenstein, J. D., and Rands, D. G., J. Am. Chem. Soc. 77 (1955) 6409.
Sela, M., and Anfinsen, C. B., Biochim. Biophys. Acta, 24 (1957) 229
Sela, M., Anfinsen, C. B., and Harrington, W. F., Biochim. Biophys. Acta, 26 (1957) 502.
Sober, H. A., Schlossman, S. F., Yaron, A., Latt, S. A., and Rushizky, G. W., Biochemistry, 5 (1966) 3608.
Lineweaver, H., and Burk, D., J. Am. Chem. Soc. 56 (1934) 658.
Michelson, A. M., J. Chem. Soc. (1959) 1371.
Zimmerman, S. B., and Sandeen, G., Anal. Biochem. 10 (1965) 444.
Michelson, A. M., Nature 181 (1958) 303.
Katchalski, E., Gehatia, M., and Sela, M., J. Am. Chem. Soc. 77 (1955) 6175.
Crestfield, A. M., Stein, W. H., and Moore, S., J. Biol. Chem. 238 (1963) 618.
Bigelow, C. C., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 31 (1960) 305.
Riehm, J. P., and Scheraga, H. A., Biochemistry, 5 (1966) 99.
Li, L. K., Riehm, J. P., and Scheraga, H. A., Biochemistry, 5 (1966) 2043.
Woody, R. W., Friedman, M. E., and Scheraga, H. A., Biochemistry, 5 (1966) 2034.
Wilchek, M., Frensdorff, A., and Sela, M., Biochemistry, 6 (1967) 247.
Trie, M., J. Biochem. (Tokyo), 57 (1965) 355.
White, F. H., Jr., Federation Proc. 20 (1961) 221.
Du, Y. C., Jiang, R. Q., and Tsou, C. L., Sci. Sinica (Peking), 14 (1965) 229.
Freedman, M. H., and Sela, M., J. Biol. Chem. 241 (1966) 2383.
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Frensdorff, A., Sela, M. (1967). Synthesis, Characterization and Enzymatic Properties of Poly-l-Lysyl Ribonuclease. In: Liébecq, C. (eds) European Journal of Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-25813-2_38
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DOI: https://doi.org/10.1007/978-3-662-25813-2_38
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