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European Journal of Biochemistry pp 267–280Cite as

Synthesis, Characterization and Enzymatic Properties of Poly-l-Lysyl Ribonuclease

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Abstract

Poly-l-lysyl RNase was prepared by the reaction of RNase with ε,N-trifluoroacetyl-α,Ncarboxy-l-lysine anhydride, either in dioxane-aqueous phosphate buffer or in dimethylformamide in the absence of phosphate. The trifluoroacetyl group was subsequently removed from polytrifluoroacetyllysyl RNase by 1.0 M aqueous piperidine. Derivatives prepared in aqueous media and enriched with up to 70 lysine residues, and derivatives prepared in dimethylformamide enriched with up to 270 lysine residues, were soluble and retained some activity. This activity could be completely recovered in all preparations after reduction of the disulfide bonds, followed by reoxidation. Approximately four phenolic hydroxyl groups were found, by spectrophotometric titration, to ionise normally in polylysyl RNase, as opposed to three in the native enzyme.

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References

  1. Herriot, R. M., Advan. Protein Chem. 3 (1947) 170.

    Google Scholar 

  2. Sri Ram, J., Bier, M., and Maurer, P. H., Advan. in Enzymol. 24 (1962) 105.

    CAS  Google Scholar 

  3. Becker, R. R., and Stahmann, M. A., J. Biol. Chem. 204 (1953) 745.

    PubMed  CAS  Google Scholar 

  4. Katchalski, E., Sela, M., Silman, H. I., and Berger, A., in The Proteins (edited by H. Neurath ), Acad. Press, New York 1964, Vol. II, p. 405.

    Google Scholar 

  5. Anfinsen, C. B., Sela, M., and Cooke, J. P., J. Biol. Chem. 237 (1962) 1825.

    PubMed  CAS  Google Scholar 

  6. Wellner, D., Silman, H. I., and Sela, M., J. Biol. Chem. 238 (1963) 1324.

    PubMed  CAS  Google Scholar 

  7. Cooke, J. P., Sela, M., and Anfinsen, C. B., J. Biol. Chem. 238 (1963) 2034.

    Google Scholar 

  8. Becker, R. R., in Polyaminoacids, Polypeptides and Proteins (edited by M. A. Stahmann), University of Wisconsin Press, Madison 1962, paper 29.

    Google Scholar 

  9. Epstein, C. J., and Goldberger, R. F., J. Biol. Chem. 239 (1964) 1087.

    Google Scholar 

  10. Becker, R. R., and Sawada, E., Federation Proc. 22 (1963) 419.

    Google Scholar 

  11. Hirs, C. H. W., Moore, S., and Stein, W. H., J. Biol. Chem. 200 (1953) 493.

    PubMed  CAS  Google Scholar 

  12. Bernfield, M. R., J. Biol. Chem. 240 (1965) 4753.

    PubMed  CAS  Google Scholar 

  13. Sela, M., Arnon, R., and Jacobson, I., Biopolymers, 1 (1963) 517.

    Article  CAS  Google Scholar 

  14. Kupke, D., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 32 (1961) 107.

    CAS  Google Scholar 

  15. Brown, D. M., Magrath, D. I., and Todd, A. R., J. Chem. Soc. (1952) 2708.

    Google Scholar 

  16. Thang, M. N., Graffe, M., and Grunberg-Manago, M., Biochim. Biophys. Acta, 108 (1965) 125.

    Article  PubMed  CAS  Google Scholar 

  17. Hauptschein, M., Stokes, C. S., and Nodiff, E. A., J. Am. Chem. Soc. 74 (1952) 4005.

    Article  CAS  Google Scholar 

  18. Anfinsen, C. B., Redfield, R. R., Choate, W. L., Page, J., and Caroll, W. R., J. Biol. Chem. 207 (1954) 201.

    PubMed  CAS  Google Scholar 

  19. Kunitz, M., J. Biol. Chem. 164 (1946) 563.

    PubMed  CAS  Google Scholar 

  20. Richards, F. M., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 29 (1955) 315.

    CAS  Google Scholar 

  21. Warner, R. C., J. Biol. Chem. 229 (1957) 717.

    Google Scholar 

  22. Kay, R. E., Harris, D. C., and Entenman, C., Arch. Biochem. Biophys. 63 (1956) 14.

    Article  PubMed  CAS  Google Scholar 

  23. Eger, C., and Yarden, A., Anal. Chem. 28 (1956) 512.

    Article  CAS  Google Scholar 

  24. Boyer, P. D., J. Am. Chem. Soc. 76 (1954) 4331.

    Article  CAS  Google Scholar 

  25. Ellman, G. L., Arch. Biochem. Biophys. 82 (1959) 70.

    Article  PubMed  CAS  Google Scholar 

  26. Spackman, D. H., Stein, W. H., and Moore, S., Anal. Chem. 30 (1958) 1190.

    Google Scholar 

  27. Yphantis, D. A., Ann. N. Y. Acad. Sci. 88 (1960) 586.

    Article  CAS  Google Scholar 

  28. Davies, B. J., Ann. N. Y. Acad. Sci. 121 (1964) 404.

    Article  Google Scholar 

  29. Anfinsen, C. B., and Haber, E., J. Biol. Chem. 236 (1960) 1361.

    Google Scholar 

  30. Epstein, C. J., Goldberger, R. F., Young, D. M., and Anfinsen, C. B., Arch. Biochem. Biophys. Supp. 1 (1962) 223.

    Google Scholar 

  31. Shapira, R., Biochem. Biophys. Res. Commun. 1 (1959) 236.

    Article  CAS  Google Scholar 

  32. Hummel, J. P., and Anderson, B. S., Arch. Biochem. Biophys. 112 (1965) 443.

    Article  PubMed  CAS  Google Scholar 

  33. Goldberger, R. F., and Anfinsen, C. B., Biochemistry, 1 (1962) 401.

    Article  CAS  Google Scholar 

  34. Hirs, C. H. W., Haimann, M., and Kycia, J. H., in Biological Structure and Function (edited by T. W. Goodwin and O. Lindberg), Academic Press, New York 1961, Vol. I.

    Google Scholar 

  35. Scheraga, H. A., and Rupley, J. A., Advan. Enzymol. 24 (1962) 161.

    CAS  Google Scholar 

  36. Harrington, W. F., and Schellman, J. A., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 30 (1956) 21.

    CAS  Google Scholar 

  37. Applequist, J. B., Ph. D. Thesis, Harvard University (1959).

    Google Scholar 

  38. Krausz, L. M., Scaduto, F. C., Sawada, F., and Becker, R. R., Federation Proc. 21 (1962) 405.

    Google Scholar 

  39. Kettman, M. S., Nishikawa, A. H., Morita, R. Y., and Becker, R. R., Biochem. Biophys. Res. Commun. 22 (1966) 262.

    Article  PubMed  CAS  Google Scholar 

  40. Shugar, D., Biochem. J. 52 (1952) 142.

    PubMed  CAS  Google Scholar 

  41. Tanford, C., Hauenstein, J. D., and Rands, D. G., J. Am. Chem. Soc. 77 (1955) 6409.

    Article  CAS  Google Scholar 

  42. Sela, M., and Anfinsen, C. B., Biochim. Biophys. Acta, 24 (1957) 229

    Article  PubMed  CAS  Google Scholar 

  43. Sela, M., Anfinsen, C. B., and Harrington, W. F., Biochim. Biophys. Acta, 26 (1957) 502.

    Article  PubMed  CAS  Google Scholar 

  44. Sober, H. A., Schlossman, S. F., Yaron, A., Latt, S. A., and Rushizky, G. W., Biochemistry, 5 (1966) 3608.

    Article  PubMed  CAS  Google Scholar 

  45. Lineweaver, H., and Burk, D., J. Am. Chem. Soc. 56 (1934) 658.

    Article  CAS  Google Scholar 

  46. Michelson, A. M., J. Chem. Soc. (1959) 1371.

    Google Scholar 

  47. Zimmerman, S. B., and Sandeen, G., Anal. Biochem. 10 (1965) 444.

    Article  CAS  Google Scholar 

  48. Michelson, A. M., Nature 181 (1958) 303.

    Article  PubMed  CAS  Google Scholar 

  49. Katchalski, E., Gehatia, M., and Sela, M., J. Am. Chem. Soc. 77 (1955) 6175.

    Article  CAS  Google Scholar 

  50. Crestfield, A. M., Stein, W. H., and Moore, S., J. Biol. Chem. 238 (1963) 618.

    PubMed  CAS  Google Scholar 

  51. Bigelow, C. C., Compt. Rend. Tray. Lab. Carlsberg, Ser. Chim. 31 (1960) 305.

    CAS  Google Scholar 

  52. Riehm, J. P., and Scheraga, H. A., Biochemistry, 5 (1966) 99.

    Article  PubMed  CAS  Google Scholar 

  53. Li, L. K., Riehm, J. P., and Scheraga, H. A., Biochemistry, 5 (1966) 2043.

    Google Scholar 

  54. Woody, R. W., Friedman, M. E., and Scheraga, H. A., Biochemistry, 5 (1966) 2034.

    Google Scholar 

  55. Wilchek, M., Frensdorff, A., and Sela, M., Biochemistry, 6 (1967) 247.

    Article  PubMed  CAS  Google Scholar 

  56. Trie, M., J. Biochem. (Tokyo), 57 (1965) 355.

    Google Scholar 

  57. White, F. H., Jr., Federation Proc. 20 (1961) 221.

    Google Scholar 

  58. Du, Y. C., Jiang, R. Q., and Tsou, C. L., Sci. Sinica (Peking), 14 (1965) 229.

    CAS  Google Scholar 

  59. Freedman, M. H., and Sela, M., J. Biol. Chem. 241 (1966) 2383.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Section of Chemical Immunology, The Weizmann Institute of Science, Rehovoth, Israel

    A. Frensdorff & M. Sela

Authors
  1. A. Frensdorff
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  2. M. Sela
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Editor information

Editors and Affiliations

  1. Liège, Belgium

    Claude Liébecq

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© 1967 Springer-Verlag Berlin Heidelberg

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Frensdorff, A., Sela, M. (1967). Synthesis, Characterization and Enzymatic Properties of Poly-l-Lysyl Ribonuclease. In: Liébecq, C. (eds) European Journal of Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-25813-2_38

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  • DOI: https://doi.org/10.1007/978-3-662-25813-2_38

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-23717-5

  • Online ISBN: 978-3-662-25813-2

  • eBook Packages: Springer Book Archive

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