Mechanism of Donor to Host Tolerance in Rat Bone Marrow Chimeras

  • P. Tutschka
  • R. Schwerdtfeger
  • R. Slavin
  • G. Santos


It is a well-established fact that individuals (animals and humans) may ultimately recover from acute graft-versus-host disease (GvHD), which complicates otherwise successful allogeneic bone marrow transplantation. Such individuals may show complete lymphohematopoietic chimerism and yet no clinical or histological evidence of continuous GvHD (2, 25, 30, 31).


Peripheral Blood Lymphocyte Transplantation Tolerance Host Tolerance Radiation Chimera Bone Marrow Cell Infusion 
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  1. (1).
    Amos, D. B., Bashir, H., Boyle, W., Mac Queen, M., and Trilikainen, A. A simple micro cytotoxicity test. Transplantation, 7: 220, 1969.PubMedCrossRefGoogle Scholar
  2. (2).
    Barnes, D. W. H., Ford, C. E., and Ilberg, P. L. T. Tolerance in the radiation chimera. Transplant. Bull., 6:101, 1958.Google Scholar
  3. (3).
    Beverly, P. C., Brent, L., Brooks, C., Medawar, P. B., and Simpson, E. In vitro reactivity of lymphoid cells from tolerant mice. Transplant. Proc., 5: 679, 1973.Google Scholar
  4. (4).
    Billingham, R. E., Brent, L., and Medawar, P. B. Quantitative studies on tissue transplantation immunity. II. Actively acquired tolerance. Philos. Trans. R. Soc. London Ser. B, 239: 357, 1956.CrossRefGoogle Scholar
  5. (5).
    Brent, L., Brooks, C. F., Lubling, N., and Thomas, A. V. Attempts to demonstrate an in vivo role for serum blocking factors in tolerance mice. Transplantation, 14:382, 1972.Google Scholar
  6. (6).
    Brent, L., Brooks, C. G., Medawar, P. B., and Simpson, E. Transplantation tolerance. Br. Med. Bull., 32:101, 1976.Google Scholar
  7. (7).
    Campbell, D. H., Garvey, Y. S., and Cremer, N. E. eds., Methods in Immunology, pp. 143–149. New York: W. A. Benjamin, 1963.Google Scholar
  8. (8).
    Cooper, M. D., Peterson, R. D. A., South, M. A., and Good, R. A. The function of the thymus system and the bursa system in the chicken. J. Exp. Med., 123:75, 1966.Google Scholar
  9. (9).
    Dorsch, S., and Roser, B. T cells mediate transplantation tolerance. Nature (London), 258: 233, 1975.CrossRefGoogle Scholar
  10. (10).
    Droege, W., and Major, K. Graft versus host reactivity and inhibitory serum factors in allografttolerant chickens. Transplantation, 19: 517, 1975.PubMedCrossRefGoogle Scholar
  11. (11).
    Elfenbein, G. J., Anderson, P. N., Humphrey, R. L., Mullins, G. M., Sensenbrenner, L. L., Wands, J. R., and Santos, G. W. Immune system reconstitution following allogeneic bone marrow transplantation in man: A multi-parameter analysis. Transplant. Proc., 18:641, 1976.Google Scholar
  12. (12).
    Elkins, W. L. The cellular basis of transplantation tolerance. Transplant. Proc., 5:685, 1973.Google Scholar
  13. (13).
    Field, E. O., Cauchi, M. N., and Gibbs, J. E. The transfer of refractoriness to graft-versus-host disease in F1 hybrid rats. Transplantation, 5: 241, 1967.CrossRefGoogle Scholar
  14. (14).
    Gengozian, N., and Urso, P. Functional activity of T and B lymphocytes in radiation chimeras. Transplant. Proc., 8:631, 1976.Google Scholar
  15. (15).
    Hellström, I., Hellström, K. E., Storb, R., and Thomas, E. D. Colony inhibition of fibroblasts from chimeric dogs mediated by the dogs’ own lymphocytes and specifically abrogated by their serum. Proc. Natl. Acad. Sci. USA, 66: 65, 1970.PubMedCrossRefGoogle Scholar
  16. (16).
    Hellström, I., Hellström, K. E., and Trentin, J. J. Cellular immunity and blocking serum activity in chimeric mice. Cell. Immunol., 7: 73, 1973.PubMedCrossRefGoogle Scholar
  17. (17).
    Heron, J. Transplantation tolerance in the rat serum—mediated? Transplantation, 15: 534, 1973.PubMedCrossRefGoogle Scholar
  18. (18).
    Humphrey, R. L., and Santos, G. W. Serum protein allotype markers in certain inbred rat strains. Fed. Proc. Fed. Ant. Soc. Exp. Biol., 30:314, 1971.Google Scholar
  19. (19).
    Miller, J. F. A., and Osaba, D. Current concepts of the immunological function of the thymus. Physiol. Rev., 47: 437, 1967.PubMedGoogle Scholar
  20. (20).
    Ochs, H. D., Storb, R., Thomas, E. D., Kolb, H. J., Graham, T. C., Mickelson, E., Parr, M., and Rudolph, R. H. Immunologic reactivity in canine marrow graft recipients. J. Immunol., 113: 1039, 1974.Google Scholar
  21. (21).
    Philips, S. M., Martin, W. J., Shaw, A. R., and Wegmann, T. G. Serum-mediated immunological non-reactivity between histoincompatible cells in tetraparental mice. Nature (London), 234: 146, 1971.CrossRefGoogle Scholar
  22. (22).
    Phillips, S. M., and Wegmann, T. G. Active suppression as a possible mechanism of tolerance in tetraparental mice. J. Exp. Med., 137:291, 1973.Google Scholar
  23. (23).
    Rouse, B. T., and Warner, N. L. Induction of T cell tolerance in agammaglobulinemic chickens. Eur. J. Immunol., 2:102, 1972.Google Scholar
  24. (24).
    Santos, G. W., and Owens, A. H., Jr. A comparison of the effects of selected cytotoxic agents on allogeneic skin graft survival in rats. Bull. Johns Hopkins Hosp., 116: 327, 1965.PubMedGoogle Scholar
  25. (25).
    Santos, G. W. Marrow transplantation in cyclophosphamide treated rats: Early donor-tohost tolerance and long lived chimeras. Exp. Hematol., 13: 36, 1967.Google Scholar
  26. (26).
    Santos, G. W., and Owens, A. H., Jr. Syngeneic and allogeneic marrow transplants in the cyclophosphamide pretreated rat. In Dausset, J., Hamburger, J., and Mathé, G., eds., Advances in Transplantation, pp. 432–436. Copenhagen: Munksgaard, 1968.Google Scholar
  27. (27).
    Santos, G. W., and Tutschka, P. J. Marrow transplantation in the busulfan-treated rat: Preclinical model of aplastic anemia. J. Natl. Cancer Inst., 53:1781, 1974.Google Scholar
  28. (28).
    Slavin, R. E., and Santos, G. W. The graft-verushost reaction in man following bone marrow transplantation: Pathology, pathogenesis, clinical features and implications. Clin. Immunol. Immunopathol., 1:472, 1973.Google Scholar
  29. (29).
    Sprent, J., vonBoehmer, H., and Nabholz, M. Association of immunity and tolerance to host H-2 determinants in irradiated F, hybrid mice reconstituted with bone marrow cells from one parental strain. J. Exp. Med., 142:321, 1975.Google Scholar
  30. (30).
    Storb, R., Rudolph, R. H., Kolb, H. J., Graham, T.Google Scholar
  31. C., Mickelson, E., Erickson, V., Lerner, K. G., Kolb, H., and Thomas, E. D. Marrow grafts between DL-A matched canine littermates. Transplantation, 15: 92, 1973.CrossRefGoogle Scholar
  32. (31).
    Thomas, E. D., Storb, R., Clift, R. A., Fefer, A., Johnson, F. L., Neiman, P. E., Lerner, K. E., Glucksberg, H., and Buckner, C. D. Bone marrow transplantation. N. Engl. J. Med., 292: 832, 1975.PubMedCrossRefGoogle Scholar
  33. (32).
    Tsoi, M. S., Storb, R., Weiden, P. L., Graham, T. C., Schroeder, M. L., and Thomas, E. D. Canine marrow transplantation: Are serum blocking factors necessary to maintain the stable chimeric state? J. Immunol., 114:531, 1975.Google Scholar
  34. (33).
    Voisin, G. A., Kinsky, R., and Maillard, Y. Protection against homologous disease in hybrid mice by passive and active immunological enhancement facilitation. Transplantation, 6:187, 1968.Google Scholar
  35. (34).
    Wegmann, T. G., Hellström, I., and Hellström, K. E. Immunological tolerance: “Forbidden Clones” allowed in tetraparental mice. Proc. Natl. Acad. Sci. USA, 68:1644, 1971.Google Scholar
  36. (35).
    Weiden, P. L., Storb, R., Tsoi, M. S., Graham, T. C., Lerner, K. G., and Thomas, E. D. Infusion of donor lymphocytes into stable canine radiation chimeras. Implications for mechanisms of transplantation tolerance. J. Immunol., 116: 1212, 1976.PubMedGoogle Scholar
  37. (36).
    Wilson, D. B., and Nowell, D. C. Quantitative studies on the mixed lymphocyte interaction in rats. IV. Immunologic potentiality of the responding cells. J. Exp. Med., 131:391, 1970.Google Scholar
  38. (37).
    Wright, P. W., Bernstein, I. D., Hamilton, D., Gluckman, J. C., and Hellström, K. E. Cell-mediated reactivity and serum blocking activity in tolerant rats. Transplantation, 18: 46, 1974.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1977

Authors and Affiliations

  • P. Tutschka
  • R. Schwerdtfeger
  • R. Slavin
  • G. Santos

There are no affiliations available

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