Colony-Forming Unit, Megakaryote (CFU-m): Its Use in Elucidating the Kinetics and Humoral Control of the Megakaryocytic Committed Progenitor Cell Compartment

  • Alexander Nakeff


The stem cell-megakaryocyte-platelet cell system is responsible for the production of blood platelets which are essential to the initiation of hemostasis and maintenance of the integrity of the blood vasculature. Platelet production is dependent upon the steady supply of megakaryocytes, which are themselves incapable of cellular proliferation, being almost exclusively in a state of cytoplasmic maturation (14). The production of megakaryocytes is thus dependent upon progenitor cells that are: (a) morphologically unidentifiable by conventional cytology; (b) committed to megakaryocyte differentiation; and (c) capable of cellular proliferation—including endoreduplication of DNA to produce polyploid cells at various ploidy levels—in addition to cell mitosis. Ultimately, of course, these progenitors must be derived through differentiation or “commitment” of pluripotent hematopoietic stem cells by a process we do not presently understand.


Mouse Bone Marrow Cytoplasmic Maturation Spleen Cell Culture Platelet Formation Femoral Marrow 
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© Springer Science+Business Media New York 1977

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  • Alexander Nakeff

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