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Cellular Structures and Nucleocytoplasmic Transport

  • Chapter
The Meaning of Nucleocytoplasmic Transport

Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

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Abstract

‘Well-characterized’ has no precise or stable agreed denotation; its semantic content, if any, derives from context. Generally it carries the connotation that good science has been done and progress has been made. Conversely, ‘X is poorly-characterized’ implies that our knowledge does not provide an adequate basis for claims that might be advanced about X; it usually heralds a skeptical assessment of someone else’s work. Sometimes these phrases are used emotively, serving only laudatory or pejorative functions. So much for precision; the point about stability is even more obvious. In the 1950s an enzyme was well-characterized if it had been purified biochemically and its kinetic properties and inhibitor sensitivities had been quantified; nowadays, sequence data, three-dimensional structure, active site chemistry and regulation of expression seem to be minimal requirements and kinetic information is of secondary interest. A method is well-characterized if it yields results that are judged reproducible, valid and interpretable; but as new methods supersede old, presumably a once-accepted procedure becomes at least relatively ill-characterized. Fashions in scientific method come and go, and as we have already seen this makes it dangerously tempting to dismiss work based on ‘outdated’ methods. Perhaps it is worth recalling that modern astronomers do not disregard Kepler, Herschel or Hubble merely because techniques have improved since then.

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  1. Napier University, Edinburgh, Scotland

    Paul S. Agutter Ph.D.

  2. Edinburgh, Scotland

    Philip L. Taylor Ph.D.

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Agutter, P.S., Taylor, P.L. (1996). Cellular Structures and Nucleocytoplasmic Transport. In: The Meaning of Nucleocytoplasmic Transport. Molecular Biology Intelligence Unit. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-22502-8_3

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