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Evidence for Ca-mediated inactivation of ICa in dialysed guinea-pig atrial cardioballs

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Adult heart muscle cells

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Summary

Ca current (ICa) was studied in cultured myocytes from right atria of adult guinea-pigs (cardioballs) by means of single low-resistance patch pipettes.

K currents were blocked by dialysis of the cells with solutions containing Cs+ as main cation and extracellular TEA (70 mM).

Under this condition membrane currents elicited by voltage clamp pulses of 200 ms in duration from −45 mV (holding potential) are net inward for depolarizations up to +55 mV without detectable contamination by outward current components. The peak inward current (ICa) has a maximum between +5 and + 10 mV and reverses around +60 mV.

Measurements of ICa tail currents obtained after clamp pulses of increasing duration to more and more positive membrane potentials suggest that ICa inactivation is not genuinely voltage-dependent.

This work was supported by the Deutsche Forschungsgemeinschaft (SFB 114, TP A8)

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References

  1. Bean BP, Nowycky WC, Tsien RW (1984): (3-adrenergic modulation of calcium channels in frog ventricular heart cells. Nature 307: 371–375

    Google Scholar 

  2. Bechem M, Pott L, Rennebaum H (1983): Atrial muscle cells from hearts of adult guinea-pigs in culture: a new preparation for cardiac cellular electrophysiology. Europ J Cell Biol 31: 366–369

    PubMed  CAS  Google Scholar 

  3. Fenwick EM, Marty A, Neher E (1982): Sodium and calcium channels in bovine chromaffin cells. J Physiol Lond 331: 599–635

    PubMed  CAS  Google Scholar 

  4. Hodgkin AC, Huxley AL (1952): A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol Lond 117: 500–544

    PubMed  CAS  Google Scholar 

  5. Isenberg G., Klöckner U (1982): Calcium currents of isolated bovine ventricular myocytes are fast and of large amplitude. Pflügers Arch 395: 30–41

    Article  PubMed  CAS  Google Scholar 

  6. Kostyuk PG,Krishtal OA, Pidoplichko VI (1976): Effect of internal fluoride and phosphate on membrane currents during intracellular dialysis of nerve cells. Nature 257: 691–693

    Article  Google Scholar 

  7. Lee KS, Akaike N, Brown AM (1980): The suction pipette method for internal perfusion and voltage clamp in small excitable cells. J Neurosci Methods 2: 58–78

    Article  Google Scholar 

  8. Lee KS, Tsien RW (1982): Reversal of current through calcium channels in dialysed single heart cells. Nature 297: 498–501

    Article  PubMed  CAS  Google Scholar 

  9. Marban E, Tsien RW (1981): Is the slow inward calcium current of heart muscle inactivated by calcium? Biophys J 33: 143a

    Google Scholar 

  10. Marty A, Neher E (1983): Tight-seal whole-cell recording. In: Sakmann B, Neher E (eds). Single channel recordings. Chapter 7, Plenum, New York, 107–1

    Chapter  Google Scholar 

  11. McDonald TF (1982): The slow inward calcium current in the heart. Ann Rev Physiol 44: 425–434

    Article  CAS  Google Scholar 

  12. Mentrard D, Vassort G, Fischmeister R (1984): Calcium-mediated inactivation of the calcium conductance in cesium-loaded frog heart cells. J Gen Physiol 83: 105–131

    Article  PubMed  CAS  Google Scholar 

  13. Nilius B, Henöek M (1983): Current dependent slow channel inactivation in heart muscle. Gen Physiol Biophys 2: 217–4

    Google Scholar 

  14. Reuter H (1973): Divalent cations as charge carriers in excitable membranes. Progr Biophys Molec Biol 26: 1–43

    Article  CAS  Google Scholar 

  15. Reuter H (1983): Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature 301: 569–574

    Article  PubMed  CAS  Google Scholar 

  16. Tillotson D (1979): Inactivation of Ca conductance dependent on entry of Ca ions in molluscan neurons. Proc Natn Acad Sci USA 77: 1497–1500

    Article  Google Scholar 

  17. Zucker R (1981): Teteraethylammonium contains an impurity which alkalizes cytoplasm and reduces calcium buffering in neurons. Brain Res 208: 473–478

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut für Zellphysiologie, Ruhr-Universität Bochum, Bochum, Germany

    L. Pott

  2. Institut für Pharmakologie, Bayer Forschungszentrum, 5600, Wuppertal, Germany

    Dr. Martin Bechem

Authors
  1. Dr. Martin Bechem
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  2. L. Pott
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Editor information

H. M. Piper P. G. Spieckermann

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© 1984 Springer-Verlag Berlin Heidelberg

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Bechem, M., Pott, L. (1984). Evidence for Ca-mediated inactivation of ICa in dialysed guinea-pig atrial cardioballs. In: Piper, H.M., Spieckermann, P.G. (eds) Adult heart muscle cells. Steinkopff, Heidelberg. https://doi.org/10.1007/978-3-662-11041-6_20

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  • DOI: https://doi.org/10.1007/978-3-662-11041-6_20

  • Publisher Name: Steinkopff, Heidelberg

  • Print ISBN: 978-3-662-11043-0

  • Online ISBN: 978-3-662-11041-6

  • eBook Packages: Springer Book Archive

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