Wheat pp 669-681 | Cite as

Cryopreservation of Germplasm of Wheat

  • Y. P. S. Bajaj
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


Germplasm resources are losing ground (Walsh 1981). With the continued enthusiastic search for obtaining novel plants and the rapid increase in the number of cultivars, it is not only becoming difficult, but at times impossible to preserve or maintain the old genetic stocks. Therefore some of the germplasm which may not be of much use at present, but might be needed in the future, is ignored and lost. Also due to indiscriminate clearing of the agricultural land there is a depletion and shrinkage of the naturally occurring germplasm pool. This has caused international concern for the conservation of rare, important, and elite germplasm.


Zygotic Embryo Germ Plasm Immature Zygotic Embryo Plant Protoplast Pollen Embryo 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bajaj YPS (1976) Gene preservation through freeze storage of cell, tissue and organs cultures. Acta Hortic 63: 75–84Google Scholar
  2. Bajaj YPS (1979a) Technology and prospects of cryopreservation of germplasm. Euphytica 28: 267–285CrossRefGoogle Scholar
  3. Bajaj YPS (1979b) Establishment of germplasm banks through freeze-storage of plant tissue culture and their implications in agriculture. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture — principles and applications. Ohio State Univ Press, Columbus. pp 745–774Google Scholar
  4. Bajaj YPS (1980) Freeze preservation of plant cells — A novel approach to the conservation of germplasm. In: Gupta AK (ed) Genetics and wheat improvement. Oxford & IBH, New Delhi pp 141–149Google Scholar
  5. Bajaj YPS (1983a) Cryopreservation and the international exchange ofgermplasm. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum, New York pp 19–41Google Scholar
  6. Bajaj YPS (1983b) Cryopreservation of germplasm of cereals — progress and prospects. In: Sakamoto S (ed) Proc 6th Int. Wheat Genetic Symp Kyoto Univ, Kyoto pp 565 574Google Scholar
  7. Bajaj YPS (1983c) Regeneration of plants from pollen-embryos of Arachis, Brassica, and Triticum spp. cryopreserved for one year. Curr Sci. 52: 484–486Google Scholar
  8. Bajaj YPS (1983d) Survival of somatic hybrid protoplasts of wheat x pea, and rice x pea subjected to -196°C. Indian J Exp Biol 21: 120–122Google Scholar
  9. Bajaj YPS (1984) The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theor Appl Genet 67: 525–528CrossRefGoogle Scholar
  10. Bajaj YPS (1985) Cryopreservation of embryos. In: Kartha K K (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton pp 227–242Google Scholar
  11. Bajaj YPS (1986a) In vitro preservation of genetic resources. Int Symp Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 43–57Google Scholar
  12. Bajaj Y PS (1986b) In vitro regeneration of diverse plants and the cryopreservation ofgermplasm in wheat (Triticum aestivum L.). Cereal Res Commun 14: 305–311Google Scholar
  13. Bajaj YPS (1988a) Biotechnology of the conservation of germplasm and its implications in agriculture and forestry. In: Proc Int Symp. Genetic manipulation in crops. IRRI, Cassell Tycooly Philadelphia, pp 125–127Google Scholar
  14. Bajaj YPS (1988b) Cryopreservation of plant protoplasts. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic engineering 1. Springer, Berlin Heidelberg New York Tokyo, pp 97–106Google Scholar
  15. Bajaj YPS (1988c) Regeneration of plants from frozen (-196°C) protoplasts of Atropa belladonna L., Datura innoxia Mill. and Nicotiana tabacum L. Indian J Exp Biol 26: 289–292Google Scholar
  16. Bajaj YPS (1989a) Induction and cryopreservation ofsomaclonal variation in wheat and rice. Int Symp Genetic manipulation in crops. CIMMYT, Mexico, pp 195–203Google Scholar
  17. Bajaj YPS (1989b) Cryopreservation of germplasm of some vegetatively propagated crops. In: Int Conf The impact of biotechnology in agriculture. Univ Amiens, FranceGoogle Scholar
  18. Bajaj YPS (ed) (1990) Biotechnology in agriculture and forestry, vol 11. Somaclonal variation in crop improvement I. Springer, Berlin Hiedelberg New York TokyoGoogle Scholar
  19. Bajaj YPS, Gosal SS (1986) Biotechnology of wheat improvement. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 2. Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 3–38Google Scholar
  20. Bajaj YPS, Gill MS, Mohapatra D (1986) Somaclonal and gametoclonal variation in wheat, cotton and brassica. In: Semai J (ed) Somaclonal variations and crop improvement. Martinus Nijhoff, Dordrecht, pp 160–169Google Scholar
  21. Chen THH, Kartha KK, Gusta LV (1985) Cryopreservation of wheat suspension culture and regenerable callus. Plant Cell Tiss Org Cult 4: 101–109CrossRefGoogle Scholar
  22. Dowgert MF, Steponkus PL (1983) Effect of cold acclimation on intracellular ice formation in isolated protoplasts. Plant Physiol 72: 978PubMedCrossRefGoogle Scholar
  23. Harrington JF (1972) Seed storage and longevity. In: Kozlowski TT (ed) Seed biology. Academic Press, New YorkGoogle Scholar
  24. Lipman CB (1936) Normal viability of seeds and bacterial spores after exposure to temperature near the absolute zero. Plant Physiol 11: 201PubMedCrossRefGoogle Scholar
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473–497CrossRefGoogle Scholar
  26. Plucknett DL, Smith NJH, Williams JT, Murthy Anishetty N (1983) Crop germplasm conservation and developing countries. Science 220: 163–169PubMedCrossRefGoogle Scholar
  27. Roberts EH (1972) Viability of seeds. Chapman and Hall, LondonCrossRefGoogle Scholar
  28. Stanwood PC (1980) Tolerance of crop seeds to cooling and storage in liquid nitrogen (-196°C). J Seed Technol 5 (1) 26Google Scholar
  29. Stanwood PC (1985) Cryopreservation of seed germplasm for genetic conservation. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 199–226Google Scholar
  30. Stanwood PC, Bass LN (1981) Seed germplasm preservation using liquid nitrogen. Seed Sci Tech 9: 423Google Scholar
  31. Steponkus PL, Dowgert MF (1981) Phenomenology of intracellular ice nucleation in isolated protoplasts. Plant Physiol 67:S-58Google Scholar
  32. Thiselton-Dryer W (1928) On the influence of the temperature of liquid hydrogen on the germinative power of seeds. R Soc Proc 62: 161Google Scholar
  33. Walsh J (1981) Germplasm resources are losing ground. Science 214: 421–423PubMedCrossRefGoogle Scholar
  34. Weber G, Roth EJ, Schweiger HG (1983) Storage of cell suspensions and protoplasts of Glycine max (L.) Merr., Brassica napus (L.), Datura innoxia (Mill.), and Daucus carota ( L.) by freezing. Z Pflanzenphysiol 109: 29–39Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Y. P. S. Bajaj
    • 1
  1. 1.Former Professor of Tissue CulturePunjab Agricultural UniversityLudhianaIndia

Personalised recommendations