Wheat pp 526-537 | Cite as

Genetics of Gliadin Proteins and the Problems of Interpreting Results Obtained with Somaclonal Variation in Wheat

  • E. V. Metakovsky
  • A. A. Sozinov
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


Seed storage alcohol-soluble protein of wheat — gliadin is apparently one of the most heterogeneous proteins in the plant kingdom. Each genotype as a rule has a unique set of gliadin polypeptides which can be separated by electrophoresis (Wrigley et al. 1982) or other appropriate methods (Bietz and Burnouf 1985). The gliadin electrophoretic spectrum, usually consisting of at least 30–50 polypeptides (Wrigley and Shepherd 1973), is constant in different environments (see Payne et al. 1984).


Somaclonal Variation Bread Wheat Cultivar Spring Bread Wheat Gliadin Protein Gliadin Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Akhmedov MG, Metakovsky EV (1987) Inheritance of gliadin components in hybrids from crossing bread wheat varieties Bezostaya 1 and Chinese Spring. Genetika (USSR) 23: 1478 - 1490Google Scholar
  2. Appleyard DB, McCausland J, Wrigley CW (1979) Checking the identity and origin of off-types in the propagation of pedigreed wheat seed. Seed Sci Technol 7: 459 - 466Google Scholar
  3. Bietz JA, Burnouf T (1985) Chromosomal control of wheat gliadin: analysis by reversed-phase H PLC. Theor Appl Genet 70: 599 - 609CrossRefGoogle Scholar
  4. Bushuk W, Zillmann RR (1978) Wheat cultivar identification by gliadin electrophoregrams. 1. Apparatus, method and nomenclature. Can J Plant Sci 58: 505-515Google Scholar
  5. Galili G. Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193: 293 - 298Google Scholar
  6. Kasarda DD (1980) Structure and properties of a-gliadins. Ann Technol Agric 29: 151 - 173Google Scholar
  7. Kreis M, Shewry PR, Forde J. Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxford Sury Plant Mol Cell BiolGoogle Scholar
  8. 2:.
  9. Larkin PJ. Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197 - 214Google Scholar
  10. Larkin PJ, Ryan SA. Brettell RIS, Scowcroft WR (1984) Heritable somaclonal variation in wheat. Theor Appl Genet 67: 443 - 455CrossRefGoogle Scholar
  11. Maddock SE, Risiott R, Parmar S, Jones MGK, Shewry PR (1985) Somaclonal variation in the gliadin patterns of grains of regenerated wheat plants. J Exp Bot 36: 1976 - 1984CrossRefGoogle Scholar
  12. Metakovsky EV, Sozinov AA (1987) Organization, variability and stability of the family of the gliadin-coding genes in wheat: genetic data. In: L$sztity R. Békés F (eds) Proc 3rd Int Worksh Gluten proteins, Budapest. May 1987, pp 30-45 (World Sci)Google Scholar
  13. Metakovsky EV, Novoselskaya A Yu, Kopus MM, Sobko TA, Sozinov AA (1984a) Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis. Theor Appl Genet 67: 559 - 568CrossRefGoogle Scholar
  14. Metakovsky EV, Novoselskaya A Yu, Sozinov AA (1984b) Genetic analysis of gliadin components in winter wheat using two-dimensional polyacryamide gel electrophoresis. Theor Appl Genet 69: 31 - 37CrossRefGoogle Scholar
  15. Metakovsky EV, Akhmedov MG. Sozinov AA (1986a) Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes. Theor Appl Genet 73:278-285Google Scholar
  16. Metakovsky EV. Koval SF, Novoselskaya A Yu, Sozinov AA (1986b) The adaptive and selective value of alleles of the gliadin-coding locus of chromosome 1 D in spring wheat studied using analysis of a hybrid population and the collection set of varieties. Genetika (USSR) 22: 843 - 850Google Scholar
  17. Metakovsky EV, Novoselskaya A Yu, Sozinov AA (1987) Problems of interpreting results obtained in studies of somaclonal variation in gliadin proteins in wheat. Theor Appl Genet 73: 764 - 766CrossRefGoogle Scholar
  18. Novoselskaya A Yu. Metakovsky EV, Sozinov AA (1983) The study of gliadin polymorphism of some wheat varieties by means of one-and two-dimensional electrophoresis. Cytol Genet 17: 45 - 49Google Scholar
  19. Novoselskaya A Yu. Upelniek VP, Sutka J, Galiba G. Metakovsky EV, Sozinov AA (1987) Studying somaclonal variation in wheat with the help of biochemical markers. In: LSsztity R. Békés F (eds) Proc 3rd Int Worksh Gluten proteins. Budapest. May 1987. pp 57-70 (World Sci)Google Scholar
  20. Payne PI. Holt LM. Jackson EA. Law CN (1984) Wheat storage proteins: their genetics and their potential for manipulation by breeding. Philos Trans R Soc London Ser B 304: 359 - 371CrossRefGoogle Scholar
  21. Pogna NE, Dal Belin Peruffo A, Boggini G. Corbellini M (1982) Analysis of wheat varieties by gliadin electrophoregrams. Nature, origin and quality of biotypes present in six Italian common wheat varieties. Genet Agric 36: 143-154Google Scholar
  22. Sobko TA (1984) Identification of blocks controlling the synthesis of the endosperm proteins in winter wheat. Visn Silskohosp Nauki 7: 78 - 80Google Scholar
  23. Sozinov AA. Poperelya FA (1980) Genetic classification of prolamines and its use for plant breeding. Ann Technol Agric 29: 229 - 245Google Scholar
  24. Sozinov AA, Poperelya FA (1984) Blocks of gliadin components in wheat genetics. breeding. and seed-growing. Kulturpflanze 32: 173 - 180Google Scholar
  25. Sozinov AA. Metakovsky EV. Koval SF (1986) Regularities of the genotype formation during selection of wheat. Vestn Selskokhoz Nauki 3: 60 - 70Google Scholar
  26. Summer-Smith M. Rafalsky JA. Sugiyama T. Stoll M, Soll D (1985) Conservation and variability of wheat a/ß-gliadin genes. Nucl Acid Res 213: 3905 - 3916Google Scholar
  27. Wall JS (1979) The role of wheat proteins in determining baking quality. In: Laidman DL. Wyn Jones RG (eds) Recent advances in the biochemistry of cereals. Academic Press. New York London, pp 275 - 311Google Scholar
  28. Wrigley CW. Shepherd KW (1973) Electrofocusing of grain proteins from wheat genotypes. Ann NY Acad Sci 209: 154 - 162Google Scholar
  29. Wrigley CW, Autran JC, Bushuk W (1982) Identification of cereal varieties by gel electrophoresis of the grain proteins. In: Pomeranz J (ed) Advances in cereal science and technology. AACC, St Paul, Minn, pp 211 - 259Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • E. V. Metakovsky
  • A. A. Sozinov
    • 1
  1. 1.Institute of General GeneticsUSSR Academy of SciencesMoscowUSSR

Personalised recommendations