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Wheat pp 481-493 | Cite as

Chromosome Instability in Bread Wheat (Triticum aestivum) Cell Suspensions and their Dividing Protoplasts

  • A. Karp
  • Q. S. Wu
  • S. E. Maddock
  • M. G. K. Jones
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)

Abstract

The development of efficient regeneration systems in the cereals is a key factor in the establishment of techniques aimed at direct manipulation of the cereal genome (Jones 1985). Regeneration has been achieved from cultured explants, particularly immature embryos and inflorescences, in a wide range of species (Maddock 1985; Tomes 1985). However, despite considerable effort, it is not yet possible to regen­erate whole plants routinely from cultured protoplasts of wheat (Triticum aestivum). This severely limits the choice of strategies for novel gene transfer by transformation and precludes protoplast fusion as a means of producing somatic hybrid plants.

Keywords

Chromosome Number Somatic Embryogenesis Bread Wheat Division Frequency Chromosome Instability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. Karp
    • 1
  • Q. S. Wu
    • 2
  • S. E. Maddock
    • 3
  • M. G. K. Jones
    • 1
  1. 1.Biochemistry DepartmentAFRC Institute of Arable Crops Research, Rothamsted Experimental StationHertsUK
  2. 2.Nanjing Agricultural UniversityNanjing, JiangsuPeoples Republic of China
  3. 3.Pioneer-Hi-Bred Int. Inc.JohnstonUSA

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