Wheat pp 481-493 | Cite as

Chromosome Instability in Bread Wheat (Triticum aestivum) Cell Suspensions and their Dividing Protoplasts

  • A. Karp
  • Q. S. Wu
  • S. E. Maddock
  • M. G. K. Jones
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


The development of efficient regeneration systems in the cereals is a key factor in the establishment of techniques aimed at direct manipulation of the cereal genome (Jones 1985). Regeneration has been achieved from cultured explants, particularly immature embryos and inflorescences, in a wide range of species (Maddock 1985; Tomes 1985). However, despite considerable effort, it is not yet possible to regen­erate whole plants routinely from cultured protoplasts of wheat (Triticum aestivum). This severely limits the choice of strategies for novel gene transfer by transformation and precludes protoplast fusion as a means of producing somatic hybrid plants.


Chromosome Number Somatic Embryogenesis Bread Wheat Division Frequency Chromosome Instability 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. Karp
    • 1
  • Q. S. Wu
    • 2
  • S. E. Maddock
    • 3
  • M. G. K. Jones
    • 1
  1. 1.Biochemistry DepartmentAFRC Institute of Arable Crops Research, Rothamsted Experimental StationHertsUK
  2. 2.Nanjing Agricultural UniversityNanjing, JiangsuPeoples Republic of China
  3. 3.Pioneer-Hi-Bred Int. Inc.JohnstonUSA

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